Fast events in protein folding: Relaxation dynamics of secondary and tertiary structure in native apomyoglobin

Rudolf Gilmanshin, Skip Williams, Robert Callender, William H. Woodruff, R. Brian Dyer

Research output: Contribution to journalArticle

204 Citations (Scopus)

Abstract

We report the fast relaxation dynamics of 'native' apomyoglobin (pH 5.3) following a 10-ns, laser-induced temperature jump. The structural dynamics are probed using time-resolved infrared spectroscopy. The infra-red kinetics monitored within the amide I absorbance of the polypeptide backbone exhibit two distinct relaxation phases which have different spectral signatures and occur on very different time scales (v = 1633 cm-1, γ = 48 ns; v = 1650 cm-1, γ = 132 μs). We assign these two spectral components to discrete substructures in the protein: helical structure that is solvated (1633 cm- 1) and native helix that is protected from solvation by interhelix tertiary interactions (1650 cm-1). Folding rate coefficients inferred from the observed relaxations at 60°C are k(f(solvated)) = (7 to 20) X 106 s-1 and k(f)(native) = 3.6 x 103 s-1, respectively. The faster rate is interpreted as the intrinsic rate of solvated helix formation, whereas the slower rate is interpreted as the rate of formation of tertiary contacts that determine a native helix. Thus, at 60°C helix formation precedes the formation of tertiary structure by over three orders of magnitude in this protein. Furthermore, the distinct thermodynamics and kinetics observed for the apomyoglobin substructures suggest that they fold independently, or quasi- independently. The observation of inhomogeneous folding for apomyoglobin is remarkable, given the relatively small size and structural simplicity of this protein.

Original languageEnglish (US)
Pages (from-to)3709-3713
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume94
Issue number8
DOIs
StatePublished - Apr 15 1997

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Protein Folding
Proteins
Thermodynamics
Amides
Spectrum Analysis
Lasers
Observation
Peptides
Temperature
apomyoglobin

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Fast events in protein folding : Relaxation dynamics of secondary and tertiary structure in native apomyoglobin. / Gilmanshin, Rudolf; Williams, Skip; Callender, Robert; Woodruff, William H.; Dyer, R. Brian.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, No. 8, 15.04.1997, p. 3709-3713.

Research output: Contribution to journalArticle

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