Abstract
Practical applications of green fluorescent protein ('GFP')-like fluorescent proteins (FPs) from species of the class Anthozoa (sea anemones, corals and sea pens) are strongly restricted owing to their oligomeric nature. Here we suggest a strategy to overcome this problem by the use of two covalently linked identical red FPs as non-oligomerizing fusion tags. We have applied this approach to the dimeric far-red fluorescent protein HcRed1 and have demonstrated superiority of the tandem tag in the in vivo labelling of fine cytoskeletal structures and tiny nucleoli. In addition, a possibility of effective fluorescence resonance energy transfer ('FRET') between enhanced yellow FP mutant ('EYFP') and tandem HcRed1 was demonstrated in a protease assay.
Original language | English (US) |
---|---|
Pages (from-to) | 17-21 |
Number of pages | 5 |
Journal | Biochemical Journal |
Volume | 368 |
Issue number | 1 |
DOIs | |
State | Published - Nov 15 2002 |
Externally published | Yes |
Keywords
- DsRed
- Green fluorescent protein (GFP)
- HcRed
- Tandem tag
ASJC Scopus subject areas
- Biochemistry
- Molecular Biology
- Cell Biology