Far-red fluorescent tag for protein labelling

Arkady F. Fradkov, Vladislav V. Verkhusha, Dmitry B. Staroverov, Maria E. Bulina, Yurii G. Yanushevich, Vladimir I. Martynov, Sergey Lukyanov, Konstantin A. Lukyanov

Research output: Contribution to journalArticle

73 Scopus citations

Abstract

Practical applications of green fluorescent protein ('GFP')-like fluorescent proteins (FPs) from species of the class Anthozoa (sea anemones, corals and sea pens) are strongly restricted owing to their oligomeric nature. Here we suggest a strategy to overcome this problem by the use of two covalently linked identical red FPs as non-oligomerizing fusion tags. We have applied this approach to the dimeric far-red fluorescent protein HcRed1 and have demonstrated superiority of the tandem tag in the in vivo labelling of fine cytoskeletal structures and tiny nucleoli. In addition, a possibility of effective fluorescence resonance energy transfer ('FRET') between enhanced yellow FP mutant ('EYFP') and tandem HcRed1 was demonstrated in a protease assay.

Original languageEnglish (US)
Pages (from-to)17-21
Number of pages5
JournalBiochemical Journal
Volume368
Issue number1
DOIs
StatePublished - Nov 15 2002
Externally publishedYes

    Fingerprint

Keywords

  • DsRed
  • Green fluorescent protein (GFP)
  • HcRed
  • Tandem tag

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Fradkov, A. F., Verkhusha, V. V., Staroverov, D. B., Bulina, M. E., Yanushevich, Y. G., Martynov, V. I., Lukyanov, S., & Lukyanov, K. A. (2002). Far-red fluorescent tag for protein labelling. Biochemical Journal, 368(1), 17-21. https://doi.org/10.1042/BJ20021191