Abstract
Determination of luminal diameter is critical to the function of small single-celled tubes. A series of EXC proteins, including EXC-1, prevent swelling of the tubular excretory canals in Caenorhabditis elegans. In this study, cloning of exc-1 reveals it to encode a homolog of mammalian IRG proteins, which play roles in immune response and autophagy and are associated with Crohn’s disease. Mutants in exc-1 accumulate early endosomes, lack recycling endosomes, and exhibit abnormal apical cytoskeletal structure in regions of enlarged tubules. EXC-1 interacts genetically with two other EXC proteins that also affect endosomal trafficking. In yeast two-hybrid assays, wild-type and putative constitutively active EXC-1 binds to the LIM-domain protein EXC-9, whose homolog, cysteine-rich intestinal protein, is enriched in mammalian intestine. These results suggest a model for IRG function in forming and maintaining apical tubule structure via regulation of endosomal recycling.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1789-1806 |
| Number of pages | 18 |
| Journal | Genetics |
| Volume | 203 |
| Issue number | 4 |
| DOIs | |
| State | Published - Aug 1 2016 |
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Keywords
- Endosomes
- Immunity-related GTPase
- IRG
- Trafficking
- Tubulogenesis
ASJC Scopus subject areas
- Genetics
Cite this
Facilitation of endosomal recycling by an IRG protein homolog maintains apical tubule structure in Caenorhabditis elegans. / Grussendorf, Kelly A.; Trezza, Christopher J.; Salem, Alexander T.; Al-Hashimi, Hikmat; Mattingly, Brendan C.; Kampmeyer, Drew E.; Khan, Liakot A.; Hall, David H.; Göbel, Verena; Ackley, Brian D.; Buechner, Matthew.
In: Genetics, Vol. 203, No. 4, 01.08.2016, p. 1789-1806.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Facilitation of endosomal recycling by an IRG protein homolog maintains apical tubule structure in Caenorhabditis elegans
AU - Grussendorf, Kelly A.
AU - Trezza, Christopher J.
AU - Salem, Alexander T.
AU - Al-Hashimi, Hikmat
AU - Mattingly, Brendan C.
AU - Kampmeyer, Drew E.
AU - Khan, Liakot A.
AU - Hall, David H.
AU - Göbel, Verena
AU - Ackley, Brian D.
AU - Buechner, Matthew
PY - 2016/8/1
Y1 - 2016/8/1
N2 - Determination of luminal diameter is critical to the function of small single-celled tubes. A series of EXC proteins, including EXC-1, prevent swelling of the tubular excretory canals in Caenorhabditis elegans. In this study, cloning of exc-1 reveals it to encode a homolog of mammalian IRG proteins, which play roles in immune response and autophagy and are associated with Crohn’s disease. Mutants in exc-1 accumulate early endosomes, lack recycling endosomes, and exhibit abnormal apical cytoskeletal structure in regions of enlarged tubules. EXC-1 interacts genetically with two other EXC proteins that also affect endosomal trafficking. In yeast two-hybrid assays, wild-type and putative constitutively active EXC-1 binds to the LIM-domain protein EXC-9, whose homolog, cysteine-rich intestinal protein, is enriched in mammalian intestine. These results suggest a model for IRG function in forming and maintaining apical tubule structure via regulation of endosomal recycling.
AB - Determination of luminal diameter is critical to the function of small single-celled tubes. A series of EXC proteins, including EXC-1, prevent swelling of the tubular excretory canals in Caenorhabditis elegans. In this study, cloning of exc-1 reveals it to encode a homolog of mammalian IRG proteins, which play roles in immune response and autophagy and are associated with Crohn’s disease. Mutants in exc-1 accumulate early endosomes, lack recycling endosomes, and exhibit abnormal apical cytoskeletal structure in regions of enlarged tubules. EXC-1 interacts genetically with two other EXC proteins that also affect endosomal trafficking. In yeast two-hybrid assays, wild-type and putative constitutively active EXC-1 binds to the LIM-domain protein EXC-9, whose homolog, cysteine-rich intestinal protein, is enriched in mammalian intestine. These results suggest a model for IRG function in forming and maintaining apical tubule structure via regulation of endosomal recycling.
KW - Endosomes
KW - Immunity-related GTPase
KW - IRG
KW - Trafficking
KW - Tubulogenesis
UR - http://www.scopus.com/inward/record.url?scp=84981489434&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84981489434&partnerID=8YFLogxK
U2 - 10.1534/genetics.116.192559
DO - 10.1534/genetics.116.192559
M3 - Article
C2 - 27334269
AN - SCOPUS:84981489434
VL - 203
SP - 1789
EP - 1806
JO - Genetics
JF - Genetics
SN - 0016-6731
IS - 4
ER -