@article{8237dfde25c4411bb6f543ef7fc83632,
title = "Facilitation of endosomal recycling by an IRG protein homolog maintains apical tubule structure in Caenorhabditis elegans",
abstract = "Determination of luminal diameter is critical to the function of small single-celled tubes. A series of EXC proteins, including EXC-1, prevent swelling of the tubular excretory canals in Caenorhabditis elegans. In this study, cloning of exc-1 reveals it to encode a homolog of mammalian IRG proteins, which play roles in immune response and autophagy and are associated with Crohn{\textquoteright}s disease. Mutants in exc-1 accumulate early endosomes, lack recycling endosomes, and exhibit abnormal apical cytoskeletal structure in regions of enlarged tubules. EXC-1 interacts genetically with two other EXC proteins that also affect endosomal trafficking. In yeast two-hybrid assays, wild-type and putative constitutively active EXC-1 binds to the LIM-domain protein EXC-9, whose homolog, cysteine-rich intestinal protein, is enriched in mammalian intestine. These results suggest a model for IRG function in forming and maintaining apical tubule structure via regulation of endosomal recycling.",
keywords = "Endosomes, IRG, Immunity-related GTPase, Trafficking, Tubulogenesis",
author = "Grussendorf, {Kelly A.} and Trezza, {Christopher J.} and Salem, {Alexander T.} and Hikmat Al-Hashimi and Mattingly, {Brendan C.} and Kampmeyer, {Drew E.} and Khan, {Liakot A.} and Hall, {David H.} and Verena G{\"o}bel and Ackley, {Brian D.} and Matthew Buechner",
note = "Funding Information: We gratefully acknowledge helpful discussions with Stuart Macdonald, Barth Grant, Erik Lundquist, Yoshiaki Azuma, Jamie Alan-Olson, Meera Sundaram, Kathy Suprenant, Robert S. Cohen, and the elusive Edward M. Hedgecock. We thank Shai Shaham for the gift of the worm strain containing amphid sheath cell marker construct nsIs53. Plasmid L3691 was a gift from A. Fire. We thank Nancy R. Hall for help with statistical analyses. Molecular graphics and analyses were performed with the University of California, San Francisco (UCSF)?s Chimera package, developed by the UCSF Resource for Biocomputing, Visualization, and Informatics (supported by National Institute of General Medical Sciences grant P41-GM-103311). Some strains were provided by the Caenorhabditis Genetics Center, which is funded by the NIH Office of Research Infrastructure Programs (grant P40-OD-010440). Parts of this work were supported by National Institutes of Health (NIH; grant R03-NS-067323). M.B. was supported by the National Science Foundation. K.A.G. was supported by the University of Kansas Graduate Research Fund award no. 2301744. C.T. was supported by the NIH Initiative for Maximizing Student Development Program grant R25-GM-62232. D.H.H. was supported by NIH grant OD-010943. Publisher Copyright: {\textcopyright} 2016 by the Genetics Society of America.",
year = "2016",
month = aug,
doi = "10.1534/genetics.116.192559",
language = "English (US)",
volume = "203",
pages = "1789--1806",
journal = "Genetics",
issn = "0016-6731",
publisher = "Genetics Society of America",
number = "4",
}