Facilitation of endosomal recycling by an IRG protein homolog maintains apical tubule structure in Caenorhabditis elegans

Kelly A. Grussendorf, Christopher J. Trezza, Alexander T. Salem, Hikmat Al-Hashimi, Brendan C. Mattingly, Drew E. Kampmeyer, Liakot A. Khan, David H. Hall, Verena Göbel, Brian D. Ackley, Matthew Buechner

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Determination of luminal diameter is critical to the function of small single-celled tubes. A series of EXC proteins, including EXC-1, prevent swelling of the tubular excretory canals in Caenorhabditis elegans. In this study, cloning of exc-1 reveals it to encode a homolog of mammalian IRG proteins, which play roles in immune response and autophagy and are associated with Crohn’s disease. Mutants in exc-1 accumulate early endosomes, lack recycling endosomes, and exhibit abnormal apical cytoskeletal structure in regions of enlarged tubules. EXC-1 interacts genetically with two other EXC proteins that also affect endosomal trafficking. In yeast two-hybrid assays, wild-type and putative constitutively active EXC-1 binds to the LIM-domain protein EXC-9, whose homolog, cysteine-rich intestinal protein, is enriched in mammalian intestine. These results suggest a model for IRG function in forming and maintaining apical tubule structure via regulation of endosomal recycling.

Original languageEnglish (US)
Pages (from-to)1789-1806
Number of pages18
JournalGenetics
Volume203
Issue number4
DOIs
StatePublished - Aug 1 2016

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Caenorhabditis elegans
Endosomes
Proteins
LIM Domain Proteins
Two-Hybrid System Techniques
Autophagy
Crohn Disease
Intestines
Cysteine
Organism Cloning

Keywords

  • Endosomes
  • Immunity-related GTPase
  • IRG
  • Trafficking
  • Tubulogenesis

ASJC Scopus subject areas

  • Genetics

Cite this

Grussendorf, K. A., Trezza, C. J., Salem, A. T., Al-Hashimi, H., Mattingly, B. C., Kampmeyer, D. E., ... Buechner, M. (2016). Facilitation of endosomal recycling by an IRG protein homolog maintains apical tubule structure in Caenorhabditis elegans. Genetics, 203(4), 1789-1806. https://doi.org/10.1534/genetics.116.192559

Facilitation of endosomal recycling by an IRG protein homolog maintains apical tubule structure in Caenorhabditis elegans. / Grussendorf, Kelly A.; Trezza, Christopher J.; Salem, Alexander T.; Al-Hashimi, Hikmat; Mattingly, Brendan C.; Kampmeyer, Drew E.; Khan, Liakot A.; Hall, David H.; Göbel, Verena; Ackley, Brian D.; Buechner, Matthew.

In: Genetics, Vol. 203, No. 4, 01.08.2016, p. 1789-1806.

Research output: Contribution to journalArticle

Grussendorf, KA, Trezza, CJ, Salem, AT, Al-Hashimi, H, Mattingly, BC, Kampmeyer, DE, Khan, LA, Hall, DH, Göbel, V, Ackley, BD & Buechner, M 2016, 'Facilitation of endosomal recycling by an IRG protein homolog maintains apical tubule structure in Caenorhabditis elegans', Genetics, vol. 203, no. 4, pp. 1789-1806. https://doi.org/10.1534/genetics.116.192559
Grussendorf KA, Trezza CJ, Salem AT, Al-Hashimi H, Mattingly BC, Kampmeyer DE et al. Facilitation of endosomal recycling by an IRG protein homolog maintains apical tubule structure in Caenorhabditis elegans. Genetics. 2016 Aug 1;203(4):1789-1806. https://doi.org/10.1534/genetics.116.192559
Grussendorf, Kelly A. ; Trezza, Christopher J. ; Salem, Alexander T. ; Al-Hashimi, Hikmat ; Mattingly, Brendan C. ; Kampmeyer, Drew E. ; Khan, Liakot A. ; Hall, David H. ; Göbel, Verena ; Ackley, Brian D. ; Buechner, Matthew. / Facilitation of endosomal recycling by an IRG protein homolog maintains apical tubule structure in Caenorhabditis elegans. In: Genetics. 2016 ; Vol. 203, No. 4. pp. 1789-1806.
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