Extremely reduced levels of heterozygosity in the vertebrate pathogen Encephalitozoon cuniculi

Mohammed Selman, Bohumil Sak, Martin Kváč, Laurent Farinelli, Louis M. Weiss, Nicolas Corradi

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

The genomes of microsporidia in the genus Encephalitozoon have been extensively studied for their minimalistic features, but they have seldom been used to investigate basic characteristics of the biology of these organisms, such as their ploidy or their mode of reproduction. In the present study, we aimed to tackle this issue by mapping Illumina sequence reads against the genomes of four strains of E. cuniculi. This approach, combined with more conventional molecular biology techniques, resulted in the identification of heterozygosity in all strains investigated, a typical signature of a diploid nuclear state. In sharp contrast with similar studies recently performed on a distant microsporidian lineage (Nematocida spp.), the level of heterozygosity that we identified across the E. cuniculi genomes was found to be extremely low. This reductive intraindividual genetic variation could result from the long-term propagation of these strains under laboratory conditions, but we propose that it could also reflect an intrinsic capacity of these vertebrate pathogens to self-reproduce.

Original languageEnglish (US)
Pages (from-to)496-502
Number of pages7
JournalEukaryotic Cell
Volume12
Issue number4
DOIs
StatePublished - Apr 2013

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Encephalitozoon cuniculi
Vertebrates
Genome
Encephalitozoon
Microsporidia
Ploidies
Diploidy
Reproduction
Molecular Biology

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

Cite this

Extremely reduced levels of heterozygosity in the vertebrate pathogen Encephalitozoon cuniculi. / Selman, Mohammed; Sak, Bohumil; Kváč, Martin; Farinelli, Laurent; Weiss, Louis M.; Corradi, Nicolas.

In: Eukaryotic Cell, Vol. 12, No. 4, 04.2013, p. 496-502.

Research output: Contribution to journalArticle

Selman, Mohammed ; Sak, Bohumil ; Kváč, Martin ; Farinelli, Laurent ; Weiss, Louis M. ; Corradi, Nicolas. / Extremely reduced levels of heterozygosity in the vertebrate pathogen Encephalitozoon cuniculi. In: Eukaryotic Cell. 2013 ; Vol. 12, No. 4. pp. 496-502.
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