Extragenic suppression of the requirement for diaminopimelate in diaminopimelate auxotrophs of Mycobacterium smegmatis

Sandra A. Consaul, William R. Jacobs, Martin S. Pavelka

Research output: Contribution to journalArticle

8 Scopus citations


Mycobacteria, like many prokaryotes, have a peptidoglycan with peptides composed of L-alanine (or glycine), D-iso-glutamine, meso-diaminopimelate, and D-alanine. We sought to study mycobacterial peptidoglycan biosynthesis by constructing diaminopimelate (DAP) auxotrophs of Mycobacterium smegmatis and then isolating spontaneous mutants of these auxotrophs that can grow in the absence of DAP. Here we report the isolation and characterization of seven classes of spontaneous M. smegmatis mutants with extragenic mutations that can suppress the DAP requirement of DAP auxotrophs.

Original languageEnglish (US)
Pages (from-to)131-135
Number of pages5
JournalFEMS Microbiology Letters
Issue number1
Publication statusPublished - Aug 8 2003



  • Diaminopimelate
  • Mycobacteria
  • Peptidoglycan

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Genetics

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