Extracellular vesicles from different pneumococcal serotypes are internalized by macrophages and induce host immune responses

Alfonso Olaya-Abril, Rafael Prados-Rosales, José A. González-Reyes, Arturo Casadevall, Liise Anne Pirofski, Manuel J. Rodríguez-Ortega

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Bacterial extracellular vesicles are membranous ultrastructures released from the cell surface. They play important roles in the interaction between the host and the bacteria. In this work, we show how extracellular vesicles produced by four different serotypes of the important human pathogen, Streptococcus pneumoniae, are internalized by murine J774A.1 macrophages via fusion with the membrane of the host cells. We also evaluated the capacity of pneumococcal extracellular vesicles to elicit an immune response by macrophages. Macrophages treated with the vesicles underwent a serotype-dependent transient loss of viability, which was further reverted. The vesicles induced the production of proinflammatory cytokines, which was higher for serotype 1 and serotype 8-derived vesicles. These results demonstrate the biological activity of extracellular vesicles of clinically important pneumococcal serotypes.

Original languageEnglish (US)
Article number1530
JournalPathogens
Volume10
Issue number12
DOIs
StatePublished - Dec 2021

Keywords

  • Host-pathogen interaction
  • Immune response
  • Membrane vesicles
  • Streptococcus pneumoniae

ASJC Scopus subject areas

  • Immunology and Allergy
  • Molecular Biology
  • Immunology and Microbiology(all)
  • Microbiology (medical)
  • Infectious Diseases

Fingerprint

Dive into the research topics of 'Extracellular vesicles from different pneumococcal serotypes are internalized by macrophages and induce host immune responses'. Together they form a unique fingerprint.

Cite this