Extracellular vesicle-mediated RNA release in Histoplasma capsulatum

Lysangela R. Alves, Roberta Peres da Silva, David A. Sanchez, Daniel Zamith-Miranda, Marcio L. Rodrigues, Samuel Goldenberg, Rosana Puccia, Joshua D. Nosanchuk

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Eukaryotic cells, including fungi, release extracellular vesicles (EVs). These lipid bilayered compartments play essential roles in cellular communication and pathogenesis. EV composition is complex and includes proteins, glycans, pigments, and RNA. RNAs with putative roles in pathogenesis have been described in EVs produced by fungi. Here we describe the RNA content in EVs produced by the G186AR and G217B strains of Histoplasma capsulatum, an important human-pathogenic fungal pathogen. A total of 124 mRNAs were identified in both strains. In this set of RNA classes, 93 transcripts were enriched in EVs from the G217B strain, whereas 31 were enriched in EVs produced by the G186AR strain. This result suggests that there are important strain-specific properties in the mRNA composition of fungal EVs. We also identified short fragments (25 to 40 nucleotides in length) that were strain specific, with a greater number identified in EVs produced by the G217B strain. Remarkably, the most highly enriched processes were stress responses and translation. Half of these fragments aligned to the reverse strand of the transcript, suggesting the occurrence of microRNA (miRNA)-like molecules in fungal EVs. We also compared the transcriptome profiles of H. capsulatum with the RNA composition of EVs, and no correlation was observed. Taking the results together, our study provided information about the RNA molecules present in H. capsulatum EVs and about the differences in composition between the strains. In addition, we found no correlation between the most highly expressed transcripts in the cell and their presence in the EVs, reinforcing the idea that the RNAs were directed to the EVs by a regulated mechanism.

Original languageEnglish (US)
Article numbere00176-19
JournalmSphere
Volume4
Issue number2
DOIs
StatePublished - Mar 1 2019

Fingerprint

Histoplasma
RNA
Extracellular Vesicles
Fungi
Messenger RNA
Eukaryotic Cells
MicroRNAs
Transcriptome
Polysaccharides

Keywords

  • Extracellular vesicles
  • Histoplasma capsulatum
  • RNA

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

Cite this

Alves, L. R., da Silva, R. P., Sanchez, D. A., Zamith-Miranda, D., Rodrigues, M. L., Goldenberg, S., ... Nosanchuk, J. D. (2019). Extracellular vesicle-mediated RNA release in Histoplasma capsulatum. mSphere, 4(2), [e00176-19]. https://doi.org/10.1128/mSphere.00176-19

Extracellular vesicle-mediated RNA release in Histoplasma capsulatum. / Alves, Lysangela R.; da Silva, Roberta Peres; Sanchez, David A.; Zamith-Miranda, Daniel; Rodrigues, Marcio L.; Goldenberg, Samuel; Puccia, Rosana; Nosanchuk, Joshua D.

In: mSphere, Vol. 4, No. 2, e00176-19, 01.03.2019.

Research output: Contribution to journalArticle

Alves, LR, da Silva, RP, Sanchez, DA, Zamith-Miranda, D, Rodrigues, ML, Goldenberg, S, Puccia, R & Nosanchuk, JD 2019, 'Extracellular vesicle-mediated RNA release in Histoplasma capsulatum', mSphere, vol. 4, no. 2, e00176-19. https://doi.org/10.1128/mSphere.00176-19
Alves LR, da Silva RP, Sanchez DA, Zamith-Miranda D, Rodrigues ML, Goldenberg S et al. Extracellular vesicle-mediated RNA release in Histoplasma capsulatum. mSphere. 2019 Mar 1;4(2). e00176-19. https://doi.org/10.1128/mSphere.00176-19
Alves, Lysangela R. ; da Silva, Roberta Peres ; Sanchez, David A. ; Zamith-Miranda, Daniel ; Rodrigues, Marcio L. ; Goldenberg, Samuel ; Puccia, Rosana ; Nosanchuk, Joshua D. / Extracellular vesicle-mediated RNA release in Histoplasma capsulatum. In: mSphere. 2019 ; Vol. 4, No. 2.
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