Extracellular proteins of Cryptococcus neoformans and host antibody response

Lin Chi Chen, Liise-anne Pirofski, Arturo Casadevall

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Proteins secreted by the fungal pathogen Cryptococcus neoformans may be involved in invasion and could be useful in vaccine design. Despite the medical importance of this fungus, little is known about its extracellular proteins or the immune response to these antigens. To study C. neoformans extracellular proteins, 12 strains were metabolically radiolabeled and protein supernatants were analyzed. Both strain- and growth condition- dependent differences were observed. Enzymatic assays of filtered culture supernatants revealed butyrate esterase and caprylate esterase lipase activity for 11 of 12 strains, as well as acid phosphatase, naphthol-AS-BI- phosphohydrolase, and β-glucosidase activities in some strains. Serum from infected rodents immunoprecipitated several secreted proteins, consistent with in vivo expression and development of an antibody response. For strain 24067, two immunodominant species, of approximately 75 and 30 kDa, were recognized. The relative intensity of the autoradiographic bands depended on the route of infection for both rats and mice. In summary, our results indicate that (i) there are multiple proteins in C. neoformans culture supernatants, (ii) there are strain differences in supernatant protein profiles, (iii) there are differences in supernatant protein profile depending on the growth conditions, (iv) there are several new extracellular and/or cell-associated enzymatic activities, and (v) antibodies to several supernatant proteins are made in the course of infection.

Original languageEnglish (US)
Pages (from-to)2599-2605
Number of pages7
JournalInfection and Immunity
Volume65
Issue number7
StatePublished - 1997

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Cryptococcus neoformans
Antibody Formation
Proteins
Caprylates
Glucosidases
Fungal Proteins
Histocompatibility Antigens Class II
Enzyme Assays
Esterases
Growth
Acid Phosphatase
Protein C
Infection
Lipase
Phosphoric Monoester Hydrolases
Rodentia
Fungi
Vaccines
Antibodies
Serum

ASJC Scopus subject areas

  • Immunology

Cite this

Extracellular proteins of Cryptococcus neoformans and host antibody response. / Chen, Lin Chi; Pirofski, Liise-anne; Casadevall, Arturo.

In: Infection and Immunity, Vol. 65, No. 7, 1997, p. 2599-2605.

Research output: Contribution to journalArticle

Chen, Lin Chi ; Pirofski, Liise-anne ; Casadevall, Arturo. / Extracellular proteins of Cryptococcus neoformans and host antibody response. In: Infection and Immunity. 1997 ; Vol. 65, No. 7. pp. 2599-2605.
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