TY - JOUR
T1 - Extracellular matrix proteoglycans and cell-substratum adhesion of human endothelial cells
T2 - the effect of methyl β-d-xylopyranoside
AU - Gordon, Portia B.
AU - Zanger, Diane R.
AU - Hatcher, Victor B.
N1 - Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 1986/8/15
Y1 - 1986/8/15
N2 - The influence of methyl β-d-xylopyranoside on human endothelial cell proteoglycans isolated from the medium and extracellular matrix was investigated. Confluent cultures of human endothelial cells incorporate significant amounts of heparan sulfate (78%), chondroitin sulfate (10%), and dermatan sulfate (12%) into the extracellular matrix. Chondroitin sulfate (35%) and dermatan sulfate (37%) were the major glycosaminoglycans present in the medium. In the presence of methyl β-d-xylopyranoside, incorporation of labeled proteoglycans into extracellular matrix was diminished by ∼ 70%. Heparan sulfate comprised the major proteoglycan present in extracellular matrix (89%) in cells grown in the presence of methyl β-d-xylopyranoside. In contrast to the incorporation of proteoglycan into extracellular matrix, methyl β-d-xylopyranoside stimulated the secretion of labeled glycosaminoglycan chains into the medium 2.5-fold. In the presence of methyl β-d-xylopyranoside, secretion of chondroitin sulfate into the medium was markedly stimulated, with a slight increase in secretion of heparan sulfate. Chondroitin sulfate (62%) and heparan sulfate (34%) were the major labele glycosaminoglycans present in medium from methyl β-d-xylopyranoside-treated cultures. Th effect of methyl β-d-xylopyranoside on cell adhesion and detachment was investigated. Cell detachment from extracellular matrix depleted of proteoglycan was significantly faster than detachment from normal matrix. Converedly, human endothelial cells adhered faster to normal matrix than to matrix depleted of proteoglycan.
AB - The influence of methyl β-d-xylopyranoside on human endothelial cell proteoglycans isolated from the medium and extracellular matrix was investigated. Confluent cultures of human endothelial cells incorporate significant amounts of heparan sulfate (78%), chondroitin sulfate (10%), and dermatan sulfate (12%) into the extracellular matrix. Chondroitin sulfate (35%) and dermatan sulfate (37%) were the major glycosaminoglycans present in the medium. In the presence of methyl β-d-xylopyranoside, incorporation of labeled proteoglycans into extracellular matrix was diminished by ∼ 70%. Heparan sulfate comprised the major proteoglycan present in extracellular matrix (89%) in cells grown in the presence of methyl β-d-xylopyranoside. In contrast to the incorporation of proteoglycan into extracellular matrix, methyl β-d-xylopyranoside stimulated the secretion of labeled glycosaminoglycan chains into the medium 2.5-fold. In the presence of methyl β-d-xylopyranoside, secretion of chondroitin sulfate into the medium was markedly stimulated, with a slight increase in secretion of heparan sulfate. Chondroitin sulfate (62%) and heparan sulfate (34%) were the major labele glycosaminoglycans present in medium from methyl β-d-xylopyranoside-treated cultures. Th effect of methyl β-d-xylopyranoside on cell adhesion and detachment was investigated. Cell detachment from extracellular matrix depleted of proteoglycan was significantly faster than detachment from normal matrix. Converedly, human endothelial cells adhered faster to normal matrix than to matrix depleted of proteoglycan.
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U2 - 10.1016/S0008-6215(00)90334-3
DO - 10.1016/S0008-6215(00)90334-3
M3 - Article
C2 - 3768884
AN - SCOPUS:0023050041
SN - 0008-6215
VL - 151
SP - 121
EP - 134
JO - Carbohydrate Research
JF - Carbohydrate Research
IS - C
ER -