Extracellular matrix heparan sulfate modulates endothelial cell susceptibility to Staphylococcus aureus

W. K. Alston, D. A. Elliott, M. E. Epstein, Victor Bernard Hatcher, M. Tang, F. D. Lowy

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The ability of extracellular matrix heparan sulfate to alter the susceptibility of human endothelial cells to S. aureus was investigated. Endothelial cells grown on extracellular matrix synthesized by S. aureus-infected endothelial cells were more susceptible to subsequent staphylococcal infection than endothelial cells grown on the extracellular matrix synthesized by untreated endothelial cells. Endothelial cells were more susceptible to S. aureus infection when 1) grown on heparitinase-treated extracellular matrix that removed heparan sulfate chains, 2) grown on extracellular matrix produced by chlorate-treated endothelial cells that reduced sulfation in the matrix heparan sulfate proteoglycans, 3) grown on heparan sulfate purified from extracellular matrix elaborated by infected endothelial cells, and 4) endothelial cells were chlorate-treated and therefore expressed desulfated cellular heparan sulfate proteoglycans. Extracellular matrix produced by S. aureus-infected endothelial cells contained heparan sulfate proteoglycans with reduced sulfation. The altered extracellular matrix with reduced sulfated heparan sulfate proteoglycans signalled the uninfected endothelial cells to produce under sulfated cellular heparan sulfate proteoglycans that increased S. aureus adherence to the endothelial cells.

Original languageEnglish (US)
Pages (from-to)102-109
Number of pages8
JournalJournal of Cellular Physiology
Volume173
Issue number1
DOIs
StatePublished - Oct 1997

Fingerprint

Heparitin Sulfate
Endothelial cells
Extracellular Matrix
Staphylococcus aureus
Endothelial Cells
Heparan Sulfate Proteoglycans
Chlorates
heparitinsulfate lyase
Staphylococcal Infections

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology
  • Physiology

Cite this

Extracellular matrix heparan sulfate modulates endothelial cell susceptibility to Staphylococcus aureus. / Alston, W. K.; Elliott, D. A.; Epstein, M. E.; Hatcher, Victor Bernard; Tang, M.; Lowy, F. D.

In: Journal of Cellular Physiology, Vol. 173, No. 1, 10.1997, p. 102-109.

Research output: Contribution to journalArticle

Alston, W. K. ; Elliott, D. A. ; Epstein, M. E. ; Hatcher, Victor Bernard ; Tang, M. ; Lowy, F. D. / Extracellular matrix heparan sulfate modulates endothelial cell susceptibility to Staphylococcus aureus. In: Journal of Cellular Physiology. 1997 ; Vol. 173, No. 1. pp. 102-109.
@article{7d7e5731135340398a39b29ab58b07f1,
title = "Extracellular matrix heparan sulfate modulates endothelial cell susceptibility to Staphylococcus aureus",
abstract = "The ability of extracellular matrix heparan sulfate to alter the susceptibility of human endothelial cells to S. aureus was investigated. Endothelial cells grown on extracellular matrix synthesized by S. aureus-infected endothelial cells were more susceptible to subsequent staphylococcal infection than endothelial cells grown on the extracellular matrix synthesized by untreated endothelial cells. Endothelial cells were more susceptible to S. aureus infection when 1) grown on heparitinase-treated extracellular matrix that removed heparan sulfate chains, 2) grown on extracellular matrix produced by chlorate-treated endothelial cells that reduced sulfation in the matrix heparan sulfate proteoglycans, 3) grown on heparan sulfate purified from extracellular matrix elaborated by infected endothelial cells, and 4) endothelial cells were chlorate-treated and therefore expressed desulfated cellular heparan sulfate proteoglycans. Extracellular matrix produced by S. aureus-infected endothelial cells contained heparan sulfate proteoglycans with reduced sulfation. The altered extracellular matrix with reduced sulfated heparan sulfate proteoglycans signalled the uninfected endothelial cells to produce under sulfated cellular heparan sulfate proteoglycans that increased S. aureus adherence to the endothelial cells.",
author = "Alston, {W. K.} and Elliott, {D. A.} and Epstein, {M. E.} and Hatcher, {Victor Bernard} and M. Tang and Lowy, {F. D.}",
year = "1997",
month = "10",
doi = "10.1002/(SICI)1097-4652(199710)173:1<102::AID-JCP12>3.0.CO;2-K",
language = "English (US)",
volume = "173",
pages = "102--109",
journal = "Journal of Cellular Physiology",
issn = "0021-9541",
publisher = "Wiley-Liss Inc.",
number = "1",

}

TY - JOUR

T1 - Extracellular matrix heparan sulfate modulates endothelial cell susceptibility to Staphylococcus aureus

AU - Alston, W. K.

AU - Elliott, D. A.

AU - Epstein, M. E.

AU - Hatcher, Victor Bernard

AU - Tang, M.

AU - Lowy, F. D.

PY - 1997/10

Y1 - 1997/10

N2 - The ability of extracellular matrix heparan sulfate to alter the susceptibility of human endothelial cells to S. aureus was investigated. Endothelial cells grown on extracellular matrix synthesized by S. aureus-infected endothelial cells were more susceptible to subsequent staphylococcal infection than endothelial cells grown on the extracellular matrix synthesized by untreated endothelial cells. Endothelial cells were more susceptible to S. aureus infection when 1) grown on heparitinase-treated extracellular matrix that removed heparan sulfate chains, 2) grown on extracellular matrix produced by chlorate-treated endothelial cells that reduced sulfation in the matrix heparan sulfate proteoglycans, 3) grown on heparan sulfate purified from extracellular matrix elaborated by infected endothelial cells, and 4) endothelial cells were chlorate-treated and therefore expressed desulfated cellular heparan sulfate proteoglycans. Extracellular matrix produced by S. aureus-infected endothelial cells contained heparan sulfate proteoglycans with reduced sulfation. The altered extracellular matrix with reduced sulfated heparan sulfate proteoglycans signalled the uninfected endothelial cells to produce under sulfated cellular heparan sulfate proteoglycans that increased S. aureus adherence to the endothelial cells.

AB - The ability of extracellular matrix heparan sulfate to alter the susceptibility of human endothelial cells to S. aureus was investigated. Endothelial cells grown on extracellular matrix synthesized by S. aureus-infected endothelial cells were more susceptible to subsequent staphylococcal infection than endothelial cells grown on the extracellular matrix synthesized by untreated endothelial cells. Endothelial cells were more susceptible to S. aureus infection when 1) grown on heparitinase-treated extracellular matrix that removed heparan sulfate chains, 2) grown on extracellular matrix produced by chlorate-treated endothelial cells that reduced sulfation in the matrix heparan sulfate proteoglycans, 3) grown on heparan sulfate purified from extracellular matrix elaborated by infected endothelial cells, and 4) endothelial cells were chlorate-treated and therefore expressed desulfated cellular heparan sulfate proteoglycans. Extracellular matrix produced by S. aureus-infected endothelial cells contained heparan sulfate proteoglycans with reduced sulfation. The altered extracellular matrix with reduced sulfated heparan sulfate proteoglycans signalled the uninfected endothelial cells to produce under sulfated cellular heparan sulfate proteoglycans that increased S. aureus adherence to the endothelial cells.

UR - http://www.scopus.com/inward/record.url?scp=0030833224&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030833224&partnerID=8YFLogxK

U2 - 10.1002/(SICI)1097-4652(199710)173:1<102::AID-JCP12>3.0.CO;2-K

DO - 10.1002/(SICI)1097-4652(199710)173:1<102::AID-JCP12>3.0.CO;2-K

M3 - Article

C2 - 9326454

AN - SCOPUS:0030833224

VL - 173

SP - 102

EP - 109

JO - Journal of Cellular Physiology

JF - Journal of Cellular Physiology

SN - 0021-9541

IS - 1

ER -