Extracellular dopamine and alterations on dopamine transporter are related to reserpine toxicity in Caenorhabditis elegans

Patrícia Reckziegel, Pan Chen, Sam Caito, Priscila Gubert, Félix Alexandre Antunes Soares, Roselei Fachinetto, Michael Aschner

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Reserpine is used as an animal model of parkinsonism. We hypothesized that the involuntary movements induced by reserpine in rodents are induced by dopaminergic toxicity caused by extracellular dopamine accumulation. The present study tested the effects of reserpine on the dopaminergic system in Caenorhabditis elegans. Reserpine was toxic to worms (decreased the survival, food intake, development and changed egg laying and defecation cycles). In addition, reserpine increased the worms’ locomotor rate on food and decreased dopamine levels. Morphological evaluations of dopaminergic CEP neurons confirmed neurodegeneration characterized by decreased fluorescence intensity and the number of worms with intact CEP neurons, and increased number of shrunken somas per worm. These effects were unrelated to reserpine’s effect on decreased expression of the dopamine transporter, dat-1. Interestingly, the locomotor rate on food and the neurodegenerative parameters fully recovered to basal conditions upon reserpine withdrawal. Furthermore, reserpine decreased survival in vesicular monoamine transporter and dat-1 loss-of-function mutant worms. In addition, worms pre-exposed to dopamine followed by exposure to reserpine had decreased survival. Reserpine activated gst-4, which controls a phase II detoxification enzymes downstream of nuclear factor (erythroid-derived-2)-like 2. Our findings establish that the dopamine transporter, dat-1, plays an important role in reserpine toxicity, likely by increasing extracellular dopamine concentrations.

Original languageEnglish (US)
Pages (from-to)633-645
Number of pages13
JournalArchives of Toxicology
Volume90
Issue number3
DOIs
StatePublished - Mar 1 2016

Fingerprint

Dopamine Plasma Membrane Transport Proteins
Reserpine
Caenorhabditis elegans
Toxicity
Dopamine
Neurons
Phase II Metabolic Detoxication
Vesicular Monoamine Transport Proteins
Food
Detoxification
Defecation
Poisons
Dopaminergic Neurons
Dyskinesias
Parkinsonian Disorders
Carisoprodol
Ovum
Rodentia
Animals
Animal Models

Keywords

  • Dopamine transporter
  • Neurodegeneration
  • Neurotoxicity
  • Parkinson’s disease
  • Worm

ASJC Scopus subject areas

  • Toxicology
  • Health, Toxicology and Mutagenesis

Cite this

Extracellular dopamine and alterations on dopamine transporter are related to reserpine toxicity in Caenorhabditis elegans. / Reckziegel, Patrícia; Chen, Pan; Caito, Sam; Gubert, Priscila; Soares, Félix Alexandre Antunes; Fachinetto, Roselei; Aschner, Michael.

In: Archives of Toxicology, Vol. 90, No. 3, 01.03.2016, p. 633-645.

Research output: Contribution to journalArticle

Reckziegel, Patrícia ; Chen, Pan ; Caito, Sam ; Gubert, Priscila ; Soares, Félix Alexandre Antunes ; Fachinetto, Roselei ; Aschner, Michael. / Extracellular dopamine and alterations on dopamine transporter are related to reserpine toxicity in Caenorhabditis elegans. In: Archives of Toxicology. 2016 ; Vol. 90, No. 3. pp. 633-645.
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