Extracellular antigen processing and presentation by immature dendritic cells

Laura Santambrogio, Aaron K. Sato, Gregory J. Carven, Svetlana L. Belyanskaya, Jack L. Strominger, Lawrence J. Stern

Research output: Contribution to journalArticle

152 Citations (Scopus)

Abstract

In antigen presentation to CD4+ T cells, proteins are degraded to peptide fragments and loaded onto class II MHC molecules in a process involving the peptide exchange factors H-2M (murine) or HLA-DM (human). In many antigen-presenting cells these processes occur in intracellular endosomal compartments, where peptides are generated and loaded onto class II MHC proteins for subsequent transport to the surface and presentation to T cells. Here, we provide evidence for an additional antigen-processing pathway in immature dendritic cells (DC). Immature DC express at the cell surface empty or peptide-receptive class II MHC molecules, as well as H-2M or HLA-DM. Secreted DC proteases act extracellularly to process intact proteins into antigenic peptides. Peptides produced by such activity are efficiently loaded onto cell surface class II MHC molecules. Together these elements comprise an unusual extracellular presentation pathway in which antigen processing and peptide loading can occur entirely outside of the cell.

Original languageEnglish (US)
Pages (from-to)15056-15061
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume96
Issue number26
DOIs
StatePublished - Dec 21 1999
Externally publishedYes

Fingerprint

Antigen Presentation
Dendritic Cells
Peptides
T-Lymphocytes
Complement Factor H
Peptide Fragments
Protein Transport
Antigen-Presenting Cells
Proteins
Peptide Hydrolases

Keywords

  • Antigen presentation
  • Class II MHC
  • H-2M
  • HLA-DM
  • Invariant chain

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Extracellular antigen processing and presentation by immature dendritic cells. / Santambrogio, Laura; Sato, Aaron K.; Carven, Gregory J.; Belyanskaya, Svetlana L.; Strominger, Jack L.; Stern, Lawrence J.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, No. 26, 21.12.1999, p. 15056-15061.

Research output: Contribution to journalArticle

Santambrogio, Laura ; Sato, Aaron K. ; Carven, Gregory J. ; Belyanskaya, Svetlana L. ; Strominger, Jack L. ; Stern, Lawrence J. / Extracellular antigen processing and presentation by immature dendritic cells. In: Proceedings of the National Academy of Sciences of the United States of America. 1999 ; Vol. 96, No. 26. pp. 15056-15061.
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