Extensive allelic variation in Cryptococcus neoformans

A. Casadevall, L. F. Freundlich, L. Marsh, Matthew D. Scharff

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

The orotidine monophosphate pyrophosphorylase (OMPPase) gene locus of the DNA of 13 Cryptococcus neoformans var. neoformans strains, including 10 recent clinical isolates, was studied by using restriction fragment length polymorphisms and nucleotide sequence analysis. The OMPPase locus (URA5) is highly polymorphic, and at least six alleles were identified. The nucleotide sequences of some alleles differed by up to 5%. The majority of the nucleotide polymorphisms in the protein-coding region occurred at the third codon position and were silent. The low frequency of replacement nucleotide substitutions relative to silent nucleotide substitutions implied that there is strong selection against amino acid changes in OMPPase. The allelic variation suggested that there is extensive genomic diversity among C. neoformans clinical isolates from one geographic area. The various alleles are potentially useful markers in the study of the population structure, epidemiology, and pathogenesis of C. neoformans strains.

Original languageEnglish (US)
Pages (from-to)1080-1084
Number of pages5
JournalJournal of Clinical Microbiology
Volume30
Issue number5
StatePublished - 1992

Fingerprint

Cryptococcus neoformans
Nucleotides
Alleles
Codon
Restriction Fragment Length Polymorphisms
Open Reading Frames
Sequence Analysis
Epidemiology
Amino Acids
DNA
Population
Genes
orotidine

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

Cite this

Casadevall, A., Freundlich, L. F., Marsh, L., & Scharff, M. D. (1992). Extensive allelic variation in Cryptococcus neoformans. Journal of Clinical Microbiology, 30(5), 1080-1084.

Extensive allelic variation in Cryptococcus neoformans. / Casadevall, A.; Freundlich, L. F.; Marsh, L.; Scharff, Matthew D.

In: Journal of Clinical Microbiology, Vol. 30, No. 5, 1992, p. 1080-1084.

Research output: Contribution to journalArticle

Casadevall, A, Freundlich, LF, Marsh, L & Scharff, MD 1992, 'Extensive allelic variation in Cryptococcus neoformans', Journal of Clinical Microbiology, vol. 30, no. 5, pp. 1080-1084.
Casadevall, A. ; Freundlich, L. F. ; Marsh, L. ; Scharff, Matthew D. / Extensive allelic variation in Cryptococcus neoformans. In: Journal of Clinical Microbiology. 1992 ; Vol. 30, No. 5. pp. 1080-1084.
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