Expression, purification, and crystallization of meso-diaminopimelate dehydrogenase from Corynebacterium glutamicum

Sreelatha G. Reddy; Giovanna Scapin; John S. Blanchard

doi:10.1002/(SICI)1097-0134(199608)25:4<514::AID-PROT12>3.3.CO;2-Y

Expression, purification, and crystallization of meso-diaminopimelate dehydrogenase from Corynebacterium glutamicum

Sreelatha G. Reddy, Giovanna Scapin, John S. Blanchard

Biochemistry

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

The gene encoding the meso-diaminopimelate dehydrogenase (DAPDH) from Corynebacterium glutamicum was overexpressed and purified to homogeneity. Crystals of the binary DAPDH-NADP⁺ complex were obtained from solutions of polyethylene glycol 8000, 100 mM sodium cacodylate, pH 6.5, and 150-300 mM Mg(OAc)₂. The crystals diffract to 2.2 Å, belong to the orthorhombic space group P2₁, and contain two molecules per asymmetric unit.

Original language	English (US)
Pages (from-to)	514-516
Number of pages	3
Journal	Proteins: Structure, Function and Genetics
Volume	25
Issue number	4
DOIs	https://doi.org/10.1002/(SICI)1097-0134(199608)25:4<514::AID-PROT12>3.3.CO;2-Y
State	Published - 1996

Keywords

D-amino acid
X-ray crystallography

ASJC Scopus subject areas

Structural Biology
Biochemistry
Molecular Biology

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10.1002/(SICI)1097-0134(199608)25:4<514::AID-PROT12>3.3.CO;2-Y

Cite this

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title = "Expression, purification, and crystallization of meso-diaminopimelate dehydrogenase from Corynebacterium glutamicum",

abstract = "The gene encoding the meso-diaminopimelate dehydrogenase (DAPDH) from Corynebacterium glutamicum was overexpressed and purified to homogeneity. Crystals of the binary DAPDH-NADP+ complex were obtained from solutions of polyethylene glycol 8000, 100 mM sodium cacodylate, pH 6.5, and 150-300 mM Mg(OAc)2. The crystals diffract to 2.2 {\AA}, belong to the orthorhombic space group P21, and contain two molecules per asymmetric unit.",

keywords = "D-amino acid, X-ray crystallography",

author = "Reddy, {Sreelatha G.} and Giovanna Scapin and Blanchard, {John S.}",

year = "1996",

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T1 - Expression, purification, and crystallization of meso-diaminopimelate dehydrogenase from Corynebacterium glutamicum

AU - Reddy, Sreelatha G.

AU - Scapin, Giovanna

AU - Blanchard, John S.

PY - 1996

Y1 - 1996

N2 - The gene encoding the meso-diaminopimelate dehydrogenase (DAPDH) from Corynebacterium glutamicum was overexpressed and purified to homogeneity. Crystals of the binary DAPDH-NADP+ complex were obtained from solutions of polyethylene glycol 8000, 100 mM sodium cacodylate, pH 6.5, and 150-300 mM Mg(OAc)2. The crystals diffract to 2.2 Å, belong to the orthorhombic space group P21, and contain two molecules per asymmetric unit.

AB - The gene encoding the meso-diaminopimelate dehydrogenase (DAPDH) from Corynebacterium glutamicum was overexpressed and purified to homogeneity. Crystals of the binary DAPDH-NADP+ complex were obtained from solutions of polyethylene glycol 8000, 100 mM sodium cacodylate, pH 6.5, and 150-300 mM Mg(OAc)2. The crystals diffract to 2.2 Å, belong to the orthorhombic space group P21, and contain two molecules per asymmetric unit.

KW - D-amino acid

KW - X-ray crystallography

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Expression, purification, and crystallization of meso-diaminopimelate dehydrogenase from Corynebacterium glutamicum

Abstract

Keywords

ASJC Scopus subject areas

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