Expression pattern of two Frizzled-related genes, Frzb-1 and Sfrp-1, during mouse embryogenesis suggests a role for modulating action of Wnt family members

Bang H. Hoang, J. Terrig Thomas, Fadi W. Abdul-Karim, Kristen M. Correia, Ronald A. Conlon, Frank P. Luyten, R. Tracy Ballock

Research output: Contribution to journalArticle

73 Citations (Scopus)

Abstract

Wnt proteins have been implicated in regulating growth and pattern formation in a variety of tissues during embryonic development. We previously identified Frzb-1, a gene which encodes a secreted protein with homology in the ligand binding domain to the Wnt receptor Frizzled, but lacking the domain encoding the putative seven transmembrane segments. Frzb-1 has recently been shown to bind to Wnt proteins in vitro, and to inhibit the activity of Xenopus Wnt-8 in vivo. We report now that mFrzb-1 and Wnt transcripts display both complementary and overlapping expression patterns at multiple sites throughout embryonic development. By Northern analysis, the expression of mFrzb-1 in the developing mouse embryo is greatest from 10.5 to 12.5 days postcoitum (dpc). In the early embryo, mFrzb-1 is expressed in the primitive streak, presomitic mesoderm, somites, and brain. Later, mFrzb-1 exhibits sharp boundaries of expression in the limb bud, branchial arches, facial mesenchyme, and in cartilaginous elements of the appendicular skeleton. We conclude from these experiments that Frzb-1 is expressed at a time and location to modulate the action of Wnt family members during development of the limbs and central nervous system.

Original languageEnglish (US)
Pages (from-to)364-372
Number of pages9
JournalDevelopmental Dynamics
Volume212
Issue number3
DOIs
StatePublished - Jul 1998
Externally publishedYes

Fingerprint

Wnt Proteins
Mesoderm
Embryonic Development
Embryonic Structures
Wnt Receptors
Primitive Streak
Branchial Region
Limb Buds
Somites
Xenopus
Skeleton
Genes
Extremities
Central Nervous System
Ligands
Brain
Growth
Proteins

Keywords

  • Embryogenesis
  • Frizzled-like proteins
  • Limb
  • Mouse
  • Wnt

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

Cite this

Expression pattern of two Frizzled-related genes, Frzb-1 and Sfrp-1, during mouse embryogenesis suggests a role for modulating action of Wnt family members. / Hoang, Bang H.; Thomas, J. Terrig; Abdul-Karim, Fadi W.; Correia, Kristen M.; Conlon, Ronald A.; Luyten, Frank P.; Ballock, R. Tracy.

In: Developmental Dynamics, Vol. 212, No. 3, 07.1998, p. 364-372.

Research output: Contribution to journalArticle

Hoang, Bang H. ; Thomas, J. Terrig ; Abdul-Karim, Fadi W. ; Correia, Kristen M. ; Conlon, Ronald A. ; Luyten, Frank P. ; Ballock, R. Tracy. / Expression pattern of two Frizzled-related genes, Frzb-1 and Sfrp-1, during mouse embryogenesis suggests a role for modulating action of Wnt family members. In: Developmental Dynamics. 1998 ; Vol. 212, No. 3. pp. 364-372.
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