Expression of the innate defense receptor S5D-SRCRB in the urogenital tract

C. Miró-Julià, C. Escoda-Ferran, E. Carrasco, J. B. Moeller, D. F. Vadekær, Xiaobo Gao, N. Paragas, J. Oliver, U. Holmskov, Q. Al-Awqati, F. Lozano

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

S5D-SRCRB is a novel mouse secretory glycoprotein belonging to the ancient and highly conserved scavenger receptor cysteine-rich superfamily of protein receptors. Available evidence indicates that S5D-SRCRB interacts with conserved microbial cell wall components, as well as with some endogenous proteins, and presents a restricted tissue expression pattern. This study further analyzes the expression of S5D-SRCRB along the mouse urogenital tract. Immunohistochemical staining for S5D-SRCRB was observed in spermatocytes from seminiferous tubules and in the epithelial surface from urethra and bladder, as well as in kidney tubules, mainly from medulla and papilla. Double stainings showed that S5D-SRCRB is expressed in both principal (P) and intercalated (IC) cells from renal collecting ducts (CD). By using an in vitro cell model of IC cell differentiation, preferential expression of S5D-SRCRB was observed in the apical border of terminally differentiated IC. Colocalization of S5D-SRCRB with galectin-3 (Gal-3) was also observed in kidney and bladder, but not in testis, supporting concurrent biochemical studies demonstrating the carbohydrate-dependent interaction of Gal-3 and S5D-SRCRB. Furthermore, upregulation of S5D-SRCRB expression was observed in in vitro and in vivo models of bacterial aggression, reinforcing the emerging view that CD, and specially IC, are important players in innate defense of the urinary tract against infection. Taken together, the results indicate that S5D-SRCRB is an integral component of the urogenital tract involved in innate immune functions.

Original languageEnglish (US)
Pages (from-to)273-285
Number of pages13
JournalTissue Antigens
Volume83
Issue number4
DOIs
StatePublished - Jan 1 2014
Externally publishedYes

Fingerprint

Galectin 3
Ducts
Urinary Bladder
Kidney Tubules
Staining and Labeling
Kidney
Scavenger Receptors
Seminiferous Tubules
Spermatocytes
Urethra
Cellular Structures
Aggression
Urinary Tract Infections
Cell Wall
Cysteine
Testis
Cell Differentiation
Glycoproteins
Proteins
Cohort Studies

Keywords

  • Collecting duct
  • Galectin-3
  • Innate immunity
  • Pathogen-associated molecular patterns
  • S5D-SRCRB
  • SSc5d

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Biochemistry
  • Genetics

Cite this

Miró-Julià, C., Escoda-Ferran, C., Carrasco, E., Moeller, J. B., Vadekær, D. F., Gao, X., ... Lozano, F. (2014). Expression of the innate defense receptor S5D-SRCRB in the urogenital tract. Tissue Antigens, 83(4), 273-285. https://doi.org/10.1111/tan.12330

Expression of the innate defense receptor S5D-SRCRB in the urogenital tract. / Miró-Julià, C.; Escoda-Ferran, C.; Carrasco, E.; Moeller, J. B.; Vadekær, D. F.; Gao, Xiaobo; Paragas, N.; Oliver, J.; Holmskov, U.; Al-Awqati, Q.; Lozano, F.

In: Tissue Antigens, Vol. 83, No. 4, 01.01.2014, p. 273-285.

Research output: Contribution to journalArticle

Miró-Julià, C, Escoda-Ferran, C, Carrasco, E, Moeller, JB, Vadekær, DF, Gao, X, Paragas, N, Oliver, J, Holmskov, U, Al-Awqati, Q & Lozano, F 2014, 'Expression of the innate defense receptor S5D-SRCRB in the urogenital tract', Tissue Antigens, vol. 83, no. 4, pp. 273-285. https://doi.org/10.1111/tan.12330
Miró-Julià C, Escoda-Ferran C, Carrasco E, Moeller JB, Vadekær DF, Gao X et al. Expression of the innate defense receptor S5D-SRCRB in the urogenital tract. Tissue Antigens. 2014 Jan 1;83(4):273-285. https://doi.org/10.1111/tan.12330
Miró-Julià, C. ; Escoda-Ferran, C. ; Carrasco, E. ; Moeller, J. B. ; Vadekær, D. F. ; Gao, Xiaobo ; Paragas, N. ; Oliver, J. ; Holmskov, U. ; Al-Awqati, Q. ; Lozano, F. / Expression of the innate defense receptor S5D-SRCRB in the urogenital tract. In: Tissue Antigens. 2014 ; Vol. 83, No. 4. pp. 273-285.
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abstract = "S5D-SRCRB is a novel mouse secretory glycoprotein belonging to the ancient and highly conserved scavenger receptor cysteine-rich superfamily of protein receptors. Available evidence indicates that S5D-SRCRB interacts with conserved microbial cell wall components, as well as with some endogenous proteins, and presents a restricted tissue expression pattern. This study further analyzes the expression of S5D-SRCRB along the mouse urogenital tract. Immunohistochemical staining for S5D-SRCRB was observed in spermatocytes from seminiferous tubules and in the epithelial surface from urethra and bladder, as well as in kidney tubules, mainly from medulla and papilla. Double stainings showed that S5D-SRCRB is expressed in both principal (P) and intercalated (IC) cells from renal collecting ducts (CD). By using an in vitro cell model of IC cell differentiation, preferential expression of S5D-SRCRB was observed in the apical border of terminally differentiated IC. Colocalization of S5D-SRCRB with galectin-3 (Gal-3) was also observed in kidney and bladder, but not in testis, supporting concurrent biochemical studies demonstrating the carbohydrate-dependent interaction of Gal-3 and S5D-SRCRB. Furthermore, upregulation of S5D-SRCRB expression was observed in in vitro and in vivo models of bacterial aggression, reinforcing the emerging view that CD, and specially IC, are important players in innate defense of the urinary tract against infection. Taken together, the results indicate that S5D-SRCRB is an integral component of the urogenital tract involved in innate immune functions.",
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