Expression of the human c-fms proto-oncogene product (colony-stimulating factor-1 receptor) on peripheral blood mononuclear cells and choriocarcinoma cell lines

C. W. Rettenmier, R. Sacca, W. L. Furman, M. F. Roussel, J. T. Holt, A. W. Nienhuis, E. R. Stanley, C. J. Sherr

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Abstract

The c-fms gene product is related, and possibly identical, to the receptor for the mononuclear phagocyte colony stimulating factor, CSF-1. Using antisera to a recombinant v-fms-coded polypeptide, glycoproteins encoded by the human c-fms locus were detected in mononuclear cells from normal peripheral blood and in promyelocytic HL-60 cells 24 h after induction of monocytic differentiation with phorbol ester. The 150-kD human c-fms-coded glycoprotein was expressed at the cell surface, was active as a tyrosine-specific protein kinase in vitro, and shared primary structural features with the product of the feline retroviral v-fms oncogene. A biochemically indistinguishable glycoprotein was detected in human choriocarcinoma cell lines. Like peripheral blood mononuclear cells and phorbol ester-treated HL-60 cells, the choriocarcinoma cells expressed high affinity binding sites for human CSF-1. In addition to serving as a lineage specific growth factor in hematopoiesis, CSF-1 may play a role in normal trophoblast development.

Original languageEnglish (US)
Pages (from-to)1740-1746
Number of pages7
JournalJournal of Clinical Investigation
Volume77
Issue number6
DOIs
StatePublished - 1986

ASJC Scopus subject areas

  • Medicine(all)

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    Rettenmier, C. W., Sacca, R., Furman, W. L., Roussel, M. F., Holt, J. T., Nienhuis, A. W., Stanley, E. R., & Sherr, C. J. (1986). Expression of the human c-fms proto-oncogene product (colony-stimulating factor-1 receptor) on peripheral blood mononuclear cells and choriocarcinoma cell lines. Journal of Clinical Investigation, 77(6), 1740-1746. https://doi.org/10.1172/JCI112496