Expression of the axolotl homologue of mouse chaperonin t-complex protein-1 during early development

Hui (Herb) Sun, Anton W. Neff, Anthony L. Mescher, George M. Malacinski

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Molecular chaperones assist in the folding of proteins, but their role during development is not well understood. Here we report the temporal and spatial expression pattern of the axolotl homologue of mouse chaperonin TCP-1 during normal amphibian embryogenesis and in several models of abnormal embryogenesis. A partial axolotl TCP-1 cDNA (646 bp; 519 coding bp) isolated by 3′ RACE PCR shows considerable homology to mouse TCP-1. Developmental Northerns and RT-PCR analyses of whole axolotl embryos revealed a low level of maternal TCP-1 transcripts in fertilized eggs. The maternal transcripts were down-regulated to a non-detectable level in early gastrulae. Zygotic TCP-1 transcripts first appeared during gastrulation. They were mainly expressed in mid-neurula and later stage embryos. Whole-mount in situ hybridization studies showed abundantTCP-1 transcripts in the blastopore at the mid-gastrula stage and in the brain and spinal cord beginning at the neurula stage, and in the somites (myotomes) at the tailbud stage. RT-PCR analysis of TCP-1 expression in axolotl embryos treated with either high salt (causing exogastrulation) or ultraviolet (UV) irradiation (causing ventralization) substantiated the correlation between TCP-1 expression and neural and somitic development. In high salt-induced exogastrulated embryos TCP-1 mRNA was detectable in the ectoderm part (with neural tissues) but not in its exogastrulated endoderm part. Lower levels of TCP-1 expression were detected in UV-irradiated, ventralized embryos with smaller head and reduced neural and somitic tissues. Normal levels of TCP-1 expression were detected in embryos with double axes/heads. These studies provide strong evidence that at the transcript level axolotl chaperonin TCP-1 is regulated both temporally and spatially during embryogenesis, especially in neural and somitic development.

Original languageEnglish (US)
Pages (from-to)157-166
Number of pages10
JournalBBA - Gene Structure and Expression
Volume1260
Issue number2
DOIs
StatePublished - Jan 25 1995
Externally publishedYes

Fingerprint

Chaperonin Containing TCP-1
Ambystoma mexicanum
Chaperonins
Embryonic Structures
Salts
Tissue
Gastrula
Molecular Chaperones
Embryonic Development
Brain
Complementary DNA
Irradiation
Polymerase Chain Reaction
Messenger RNA
Head
Mothers
Somites
Gastrulation
Endoderm
Ectoderm

Keywords

  • Axolotl
  • Chaperonin
  • Embryogenesis
  • Embryonic expression
  • t-complex protein 1 (TCP-1)

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Genetics
  • Molecular Biology
  • Structural Biology

Cite this

Expression of the axolotl homologue of mouse chaperonin t-complex protein-1 during early development. / Sun, Hui (Herb); Neff, Anton W.; Mescher, Anthony L.; Malacinski, George M.

In: BBA - Gene Structure and Expression, Vol. 1260, No. 2, 25.01.1995, p. 157-166.

Research output: Contribution to journalArticle

Sun, Hui (Herb) ; Neff, Anton W. ; Mescher, Anthony L. ; Malacinski, George M. / Expression of the axolotl homologue of mouse chaperonin t-complex protein-1 during early development. In: BBA - Gene Structure and Expression. 1995 ; Vol. 1260, No. 2. pp. 157-166.
@article{89817ba27f994569b65d707cdf913969,
title = "Expression of the axolotl homologue of mouse chaperonin t-complex protein-1 during early development",
abstract = "Molecular chaperones assist in the folding of proteins, but their role during development is not well understood. Here we report the temporal and spatial expression pattern of the axolotl homologue of mouse chaperonin TCP-1 during normal amphibian embryogenesis and in several models of abnormal embryogenesis. A partial axolotl TCP-1 cDNA (646 bp; 519 coding bp) isolated by 3′ RACE PCR shows considerable homology to mouse TCP-1. Developmental Northerns and RT-PCR analyses of whole axolotl embryos revealed a low level of maternal TCP-1 transcripts in fertilized eggs. The maternal transcripts were down-regulated to a non-detectable level in early gastrulae. Zygotic TCP-1 transcripts first appeared during gastrulation. They were mainly expressed in mid-neurula and later stage embryos. Whole-mount in situ hybridization studies showed abundantTCP-1 transcripts in the blastopore at the mid-gastrula stage and in the brain and spinal cord beginning at the neurula stage, and in the somites (myotomes) at the tailbud stage. RT-PCR analysis of TCP-1 expression in axolotl embryos treated with either high salt (causing exogastrulation) or ultraviolet (UV) irradiation (causing ventralization) substantiated the correlation between TCP-1 expression and neural and somitic development. In high salt-induced exogastrulated embryos TCP-1 mRNA was detectable in the ectoderm part (with neural tissues) but not in its exogastrulated endoderm part. Lower levels of TCP-1 expression were detected in UV-irradiated, ventralized embryos with smaller head and reduced neural and somitic tissues. Normal levels of TCP-1 expression were detected in embryos with double axes/heads. These studies provide strong evidence that at the transcript level axolotl chaperonin TCP-1 is regulated both temporally and spatially during embryogenesis, especially in neural and somitic development.",
keywords = "Axolotl, Chaperonin, Embryogenesis, Embryonic expression, t-complex protein 1 (TCP-1)",
author = "Sun, {Hui (Herb)} and Neff, {Anton W.} and Mescher, {Anthony L.} and Malacinski, {George M.}",
year = "1995",
month = "1",
day = "25",
doi = "10.1016/0167-4781(94)00187-8",
language = "English (US)",
volume = "1260",
pages = "157--166",
journal = "Biochimica et Biophysica Acta - Gene Structure and Expression",
issn = "0167-4781",
publisher = "Elsevier BV",
number = "2",

}

TY - JOUR

T1 - Expression of the axolotl homologue of mouse chaperonin t-complex protein-1 during early development

AU - Sun, Hui (Herb)

AU - Neff, Anton W.

AU - Mescher, Anthony L.

AU - Malacinski, George M.

PY - 1995/1/25

Y1 - 1995/1/25

N2 - Molecular chaperones assist in the folding of proteins, but their role during development is not well understood. Here we report the temporal and spatial expression pattern of the axolotl homologue of mouse chaperonin TCP-1 during normal amphibian embryogenesis and in several models of abnormal embryogenesis. A partial axolotl TCP-1 cDNA (646 bp; 519 coding bp) isolated by 3′ RACE PCR shows considerable homology to mouse TCP-1. Developmental Northerns and RT-PCR analyses of whole axolotl embryos revealed a low level of maternal TCP-1 transcripts in fertilized eggs. The maternal transcripts were down-regulated to a non-detectable level in early gastrulae. Zygotic TCP-1 transcripts first appeared during gastrulation. They were mainly expressed in mid-neurula and later stage embryos. Whole-mount in situ hybridization studies showed abundantTCP-1 transcripts in the blastopore at the mid-gastrula stage and in the brain and spinal cord beginning at the neurula stage, and in the somites (myotomes) at the tailbud stage. RT-PCR analysis of TCP-1 expression in axolotl embryos treated with either high salt (causing exogastrulation) or ultraviolet (UV) irradiation (causing ventralization) substantiated the correlation between TCP-1 expression and neural and somitic development. In high salt-induced exogastrulated embryos TCP-1 mRNA was detectable in the ectoderm part (with neural tissues) but not in its exogastrulated endoderm part. Lower levels of TCP-1 expression were detected in UV-irradiated, ventralized embryos with smaller head and reduced neural and somitic tissues. Normal levels of TCP-1 expression were detected in embryos with double axes/heads. These studies provide strong evidence that at the transcript level axolotl chaperonin TCP-1 is regulated both temporally and spatially during embryogenesis, especially in neural and somitic development.

AB - Molecular chaperones assist in the folding of proteins, but their role during development is not well understood. Here we report the temporal and spatial expression pattern of the axolotl homologue of mouse chaperonin TCP-1 during normal amphibian embryogenesis and in several models of abnormal embryogenesis. A partial axolotl TCP-1 cDNA (646 bp; 519 coding bp) isolated by 3′ RACE PCR shows considerable homology to mouse TCP-1. Developmental Northerns and RT-PCR analyses of whole axolotl embryos revealed a low level of maternal TCP-1 transcripts in fertilized eggs. The maternal transcripts were down-regulated to a non-detectable level in early gastrulae. Zygotic TCP-1 transcripts first appeared during gastrulation. They were mainly expressed in mid-neurula and later stage embryos. Whole-mount in situ hybridization studies showed abundantTCP-1 transcripts in the blastopore at the mid-gastrula stage and in the brain and spinal cord beginning at the neurula stage, and in the somites (myotomes) at the tailbud stage. RT-PCR analysis of TCP-1 expression in axolotl embryos treated with either high salt (causing exogastrulation) or ultraviolet (UV) irradiation (causing ventralization) substantiated the correlation between TCP-1 expression and neural and somitic development. In high salt-induced exogastrulated embryos TCP-1 mRNA was detectable in the ectoderm part (with neural tissues) but not in its exogastrulated endoderm part. Lower levels of TCP-1 expression were detected in UV-irradiated, ventralized embryos with smaller head and reduced neural and somitic tissues. Normal levels of TCP-1 expression were detected in embryos with double axes/heads. These studies provide strong evidence that at the transcript level axolotl chaperonin TCP-1 is regulated both temporally and spatially during embryogenesis, especially in neural and somitic development.

KW - Axolotl

KW - Chaperonin

KW - Embryogenesis

KW - Embryonic expression

KW - t-complex protein 1 (TCP-1)

UR - http://www.scopus.com/inward/record.url?scp=0028952375&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028952375&partnerID=8YFLogxK

U2 - 10.1016/0167-4781(94)00187-8

DO - 10.1016/0167-4781(94)00187-8

M3 - Article

VL - 1260

SP - 157

EP - 166

JO - Biochimica et Biophysica Acta - Gene Structure and Expression

JF - Biochimica et Biophysica Acta - Gene Structure and Expression

SN - 0167-4781

IS - 2

ER -