Abstract
Movement of pests to non-host crops due to resistance of their host plants is discussed. We report the simultaneous control of the European corn borer (Ostrinia nubilalis) and protection of transgenic rice (Oryza sativa) from the damages of this non-host pest. Indica rice Basmati 370 variety was genetically engineered via the Agrobacterium-mediated system for expression of Bacillus thuringiensis (cry1Ab and cry1Ac), hygromycin resistance (hpt) and β-glucuronidase (gus) genes. Molecular analysis of R0 and R1 progeny plants confirmed the integration, transcription and translation of all transgenes, with a 100% cointegration of linked genes. Southern blot analysis revealed the integration of transgenes in the range of one to three copy numbers. Northern blots revealed the presence of intact, full-length transcripts of the cry1Ab and cry1Ac. The expression of Cry1Ab and Cry1Ac was estimated up to 0.1% of total soluble protein. Inheritance of the introduced genes to R1 progeny was found to be in agreement with the Mendelian ratio in most of the transgenic lines. Bioassays, feeding of R1 progeny, of four independent transgenic lines, showed high levels of resistance to the European corn borer. Transgenic lines showed 100% mortality 5 d after the infestation in the bioassay experiments.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 213-220 |
| Number of pages | 8 |
| Journal | In Vitro Cellular and Developmental Biology - Plant |
| Volume | 38 |
| Issue number | 2 |
| DOIs | |
| State | Published - 2002 |
| Externally published | Yes |
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Keywords
- Bacillus thuringiensis
- Feeding assays
- Gus
- Hpt
ASJC Scopus subject areas
- Plant Science
- Cell Biology
- Developmental Biology
- Embryology
Cite this
Expression of synthetic cry1Ab and cry1Ac genes in Basmati rice (Oryza sativa l.) Variety 370 via Agrobacterium-mediated transformation for the control of the European corn borer (Ostrinia nubilalis). / Ahmad, Anwaar; Maqbool, Shahina B.; Riazuddin, Sheikh; Sticklen, Mariam B.
In: In Vitro Cellular and Developmental Biology - Plant, Vol. 38, No. 2, 2002, p. 213-220.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Expression of synthetic cry1Ab and cry1Ac genes in Basmati rice (Oryza sativa l.) Variety 370 via Agrobacterium-mediated transformation for the control of the European corn borer (Ostrinia nubilalis)
AU - Ahmad, Anwaar
AU - Maqbool, Shahina B.
AU - Riazuddin, Sheikh
AU - Sticklen, Mariam B.
PY - 2002
Y1 - 2002
N2 - Movement of pests to non-host crops due to resistance of their host plants is discussed. We report the simultaneous control of the European corn borer (Ostrinia nubilalis) and protection of transgenic rice (Oryza sativa) from the damages of this non-host pest. Indica rice Basmati 370 variety was genetically engineered via the Agrobacterium-mediated system for expression of Bacillus thuringiensis (cry1Ab and cry1Ac), hygromycin resistance (hpt) and β-glucuronidase (gus) genes. Molecular analysis of R0 and R1 progeny plants confirmed the integration, transcription and translation of all transgenes, with a 100% cointegration of linked genes. Southern blot analysis revealed the integration of transgenes in the range of one to three copy numbers. Northern blots revealed the presence of intact, full-length transcripts of the cry1Ab and cry1Ac. The expression of Cry1Ab and Cry1Ac was estimated up to 0.1% of total soluble protein. Inheritance of the introduced genes to R1 progeny was found to be in agreement with the Mendelian ratio in most of the transgenic lines. Bioassays, feeding of R1 progeny, of four independent transgenic lines, showed high levels of resistance to the European corn borer. Transgenic lines showed 100% mortality 5 d after the infestation in the bioassay experiments.
AB - Movement of pests to non-host crops due to resistance of their host plants is discussed. We report the simultaneous control of the European corn borer (Ostrinia nubilalis) and protection of transgenic rice (Oryza sativa) from the damages of this non-host pest. Indica rice Basmati 370 variety was genetically engineered via the Agrobacterium-mediated system for expression of Bacillus thuringiensis (cry1Ab and cry1Ac), hygromycin resistance (hpt) and β-glucuronidase (gus) genes. Molecular analysis of R0 and R1 progeny plants confirmed the integration, transcription and translation of all transgenes, with a 100% cointegration of linked genes. Southern blot analysis revealed the integration of transgenes in the range of one to three copy numbers. Northern blots revealed the presence of intact, full-length transcripts of the cry1Ab and cry1Ac. The expression of Cry1Ab and Cry1Ac was estimated up to 0.1% of total soluble protein. Inheritance of the introduced genes to R1 progeny was found to be in agreement with the Mendelian ratio in most of the transgenic lines. Bioassays, feeding of R1 progeny, of four independent transgenic lines, showed high levels of resistance to the European corn borer. Transgenic lines showed 100% mortality 5 d after the infestation in the bioassay experiments.
KW - Bacillus thuringiensis
KW - Feeding assays
KW - Gus
KW - Hpt
UR - http://www.scopus.com/inward/record.url?scp=0036242957&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0036242957&partnerID=8YFLogxK
U2 - 10.1079/IVPIVP2001277
DO - 10.1079/IVPIVP2001277
M3 - Article
AN - SCOPUS:0036242957
VL - 38
SP - 213
EP - 220
JO - In Vitro Cellular and Developmental Biology - Plant
JF - In Vitro Cellular and Developmental Biology - Plant
SN - 1054-5476
IS - 2
ER -