Expression of sodium-independent organic anion uptake systems of skate liver in Xenopus laevis oocytes

E. Jacquemin, B. Hagenbuch, Allan W. Wolkoff, P. J. Meier, J. L. Boyer

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Abstract

The expression of the basolateral sodium-independent organic onion uptake system of the little skate (Raja erinacea) has been studied in Xenopus laevis oocytes. Injection of oocytes with skate liver poly(A)+ RNA resulted in the functional expression of chloride-dependent sulfobromophthalein (BSP) uptake and sodium-independent taurocholate uptake within 3-5 days. The expressed chloride-dependent BSP uptake activity exhibited saturation kinetics [apparent Michaelis constant (K(m)) 1.8 μM] and efficiently extracted BSP from its binding sites on bovine serum albumin. The chloride-sensitive portion of BSP uptake was inhibited by bilirubin (10 μM; 27%), 4,4'- diisothiocyanostilbene-2,2'-disulfonic acid (100 μM; 57%), bumetanide (100 μM; 48%), taurocholate (200 μM; 515), and cholate (200 μM; 45%). Size fractionation of total skate liver mRNA revealed that a 1.8- to 2.9-kb size class mRNA was sufficient to express chloride-dependent BSP uptake and sodium-independent taurocholate uptake. In addition, a 1- to 1.7-kb size class mRNA expressed sodium-independent taurocholate uptake but had no effects on BSP uptake. This study confirms that an organic onion transport system for chloride-dependent BSP uptake, with characteristics similar to rat liver, is already expressed in the liver of lower vertebrates and thus represents a phylogenetically old system. Sodium-independent taurocholate uptake in skate liver may be mediated by two different transport systems.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Gastrointestinal and Liver Physiology
Volume268
Issue number1 31-1
StatePublished - 1995
Externally publishedYes

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Taurocholic Acid
Rajidae
Xenopus laevis
anions
Oocytes
Anions
Chlorides
chlorides
oocytes
Sodium
sodium
liver
Leucoraja erinacea
Liver
Messenger RNA
Onions
onions
Skates (Fish)
Sulfobromophthalein
Cholates

ASJC Scopus subject areas

  • Physiology
  • Gastroenterology
  • Agricultural and Biological Sciences(all)

Cite this

Expression of sodium-independent organic anion uptake systems of skate liver in Xenopus laevis oocytes. / Jacquemin, E.; Hagenbuch, B.; Wolkoff, Allan W.; Meier, P. J.; Boyer, J. L.

In: American Journal of Physiology - Gastrointestinal and Liver Physiology, Vol. 268, No. 1 31-1, 1995.

Research output: Contribution to journalArticle

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AU - Boyer, J. L.

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