Expression of selenium-binding protein 1 characterizes intestinal cell maturation and predicts survival for patients with colorectal cancer

Tianhong Li, Wancai Yang, Maomi Li, Do Sun Byun, Chang Tong, Shannon Nasser, Min Zhuang, Diego Arango, John M. Mariadason, Leonard H. Augenlicht

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

To identify candidate genes involved in the development of colorectal cancer, we used cDNA microarrays to analyze gene expression differences between human colorectal tumors and paired adjacent normal mucosa. We identified ∼3.5-fold significant downregulation of selenium-binding protein 1 (SBP1) in colorectal tumors compared to normal mucosa (p = 0.003). Importantly, stage III colorectal cancer patients with low tumor-SBP1 expression had significantly shorter disease-free and overall survival as compared with those patients with high tumor-SBP1 expression (p = 0.04 and 0.03, respectively). We further characterized the role of SBP1 in colorectal cancer in vivo and in vitro. In normal tissue, SBP1 was maximally expressed in terminally differentiated epithelial cells on the luminal surface of crypts in the large intestine. Consistent with this in vivo localization, SBP1 was upregulated during in vitro colonic cell differentiation along the absorptive (Caco-2) and secretory (HT29 Clones 16E and 19A) cell lineages. Downregulation (approximately 50%) of SBP1 expression by small interfering RNA in colonic cancer cells was associated with reduced expression of another epithelial differentiation marker, carcinoembryonic antigen (CEA), although PCNA and p21WAF1/cip1 expression were not altered. These data demonstrate that higher expression of SBP1 is associated with differentiation of the normal colonic epithelia and may be a positive prognostic factor for survival in stage III colorectal carcinoma.

Original languageEnglish (US)
Pages (from-to)1289-1299
Number of pages11
JournalMolecular Nutrition and Food Research
Volume52
Issue number11
DOIs
StatePublished - Nov 2008

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Selenium-Binding Proteins
colorectal neoplasms
selenium
binding proteins
Colorectal Neoplasms
Survival
cells
Differentiation Antigens
mucosa
Mucous Membrane
Down-Regulation
neoplasms
Large Intestine
Carcinoembryonic Antigen
Proliferating Cell Nuclear Antigen
Cell Lineage
large intestine
Microarray Analysis
small interfering RNA
Oligonucleotide Array Sequence Analysis

Keywords

  • Colorectal cancer
  • Human selenium-binding protein 1
  • Intestinal cell differentiation
  • Intestinal cell maturation
  • Prognostic factor

ASJC Scopus subject areas

  • Food Science
  • Biotechnology

Cite this

Expression of selenium-binding protein 1 characterizes intestinal cell maturation and predicts survival for patients with colorectal cancer. / Li, Tianhong; Yang, Wancai; Li, Maomi; Byun, Do Sun; Tong, Chang; Nasser, Shannon; Zhuang, Min; Arango, Diego; Mariadason, John M.; Augenlicht, Leonard H.

In: Molecular Nutrition and Food Research, Vol. 52, No. 11, 11.2008, p. 1289-1299.

Research output: Contribution to journalArticle

Li, Tianhong ; Yang, Wancai ; Li, Maomi ; Byun, Do Sun ; Tong, Chang ; Nasser, Shannon ; Zhuang, Min ; Arango, Diego ; Mariadason, John M. ; Augenlicht, Leonard H. / Expression of selenium-binding protein 1 characterizes intestinal cell maturation and predicts survival for patients with colorectal cancer. In: Molecular Nutrition and Food Research. 2008 ; Vol. 52, No. 11. pp. 1289-1299.
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