Expression of recombinant actin 5C from drosophila in the methylotrophic yeast pichia pastoris

O. V. Nevzglyadova; A. V. Artemov; V. V. Zenin; V. V. Verkhusha; M. M. Shavlovsky; O. I. Povarova; Olesya V. Stepanenko; I. M. Kuznetsova; K. K. Turoverov

Expression of recombinant actin 5C from drosophila in the methylotrophic yeast pichia pastoris

O. V. Nevzglyadova, A. V. Artemov, V. V. Zenin, V. V. Verkhusha, M. M. Shavlovsky, O. I. Povarova, Olesya V. Stepanenko, I. M. Kuznetsova, K. K. Turoverov

Research output: Contribution to journal › Article › peer-review

Abstract

A system for actin expression in cells of yeast Pichia pastoris was constructed. Drosophila actin 5C, by 90% homologous to β-actin of higher eukaryotes, was used as a target protein. To improve the procedures of target protein biosynthesis in yeast cells and of extraction and purification of recombinant actin the fusion protein GFP-actin 5C, having fluorescence protein GFP as a reporter part, was expressed and purified. The dimensions and resistance of yeast cells producing recombinant actin were characterized. It was shown that the size and form of cells depended on the accumulation of recombinant protein. The purified fusion protein was used for obtaining polyclonal antibody for testing recombinant actin.

Original language	English (US)
Pages (from-to)	300-310
Number of pages	11
Journal	Tsitologiya
Volume	49
Issue number	4
State	Published - 2007
Externally published	Yes

Keywords

Actin cytoskeleton
GFP-actin fusion
Green fluorescent protein
Pichia pastoris
Recombinant actin

ASJC Scopus subject areas

Pathology and Forensic Medicine
Histology

Cite this

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title = "Expression of recombinant actin 5C from drosophila in the methylotrophic yeast pichia pastoris",

abstract = "A system for actin expression in cells of yeast Pichia pastoris was constructed. Drosophila actin 5C, by 90% homologous to β-actin of higher eukaryotes, was used as a target protein. To improve the procedures of target protein biosynthesis in yeast cells and of extraction and purification of recombinant actin the fusion protein GFP-actin 5C, having fluorescence protein GFP as a reporter part, was expressed and purified. The dimensions and resistance of yeast cells producing recombinant actin were characterized. It was shown that the size and form of cells depended on the accumulation of recombinant protein. The purified fusion protein was used for obtaining polyclonal antibody for testing recombinant actin.",

keywords = "Actin cytoskeleton, GFP-actin fusion, Green fluorescent protein, Pichia pastoris, Recombinant actin",

author = "Nevzglyadova, {O. V.} and Artemov, {A. V.} and Zenin, {V. V.} and Verkhusha, {V. V.} and Shavlovsky, {M. M.} and Povarova, {O. I.} and Stepanenko, {Olesya V.} and Kuznetsova, {I. M.} and Turoverov, {K. K.}",

year = "2007",

language = "English (US)",

volume = "49",

pages = "300--310",

journal = "Tsitologiya",

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TY - JOUR

T1 - Expression of recombinant actin 5C from drosophila in the methylotrophic yeast pichia pastoris

AU - Nevzglyadova, O. V.

AU - Artemov, A. V.

AU - Zenin, V. V.

AU - Verkhusha, V. V.

AU - Shavlovsky, M. M.

AU - Povarova, O. I.

AU - Stepanenko, Olesya V.

AU - Kuznetsova, I. M.

AU - Turoverov, K. K.

PY - 2007

Y1 - 2007

N2 - A system for actin expression in cells of yeast Pichia pastoris was constructed. Drosophila actin 5C, by 90% homologous to β-actin of higher eukaryotes, was used as a target protein. To improve the procedures of target protein biosynthesis in yeast cells and of extraction and purification of recombinant actin the fusion protein GFP-actin 5C, having fluorescence protein GFP as a reporter part, was expressed and purified. The dimensions and resistance of yeast cells producing recombinant actin were characterized. It was shown that the size and form of cells depended on the accumulation of recombinant protein. The purified fusion protein was used for obtaining polyclonal antibody for testing recombinant actin.

AB - A system for actin expression in cells of yeast Pichia pastoris was constructed. Drosophila actin 5C, by 90% homologous to β-actin of higher eukaryotes, was used as a target protein. To improve the procedures of target protein biosynthesis in yeast cells and of extraction and purification of recombinant actin the fusion protein GFP-actin 5C, having fluorescence protein GFP as a reporter part, was expressed and purified. The dimensions and resistance of yeast cells producing recombinant actin were characterized. It was shown that the size and form of cells depended on the accumulation of recombinant protein. The purified fusion protein was used for obtaining polyclonal antibody for testing recombinant actin.

KW - Actin cytoskeleton

KW - GFP-actin fusion

KW - Green fluorescent protein

KW - Pichia pastoris

KW - Recombinant actin

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Expression of recombinant actin 5C from drosophila in the methylotrophic yeast pichia pastoris

Abstract

Keywords

ASJC Scopus subject areas

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