Expression of protease and protease-inhibitory activity in human mononuclear leukocyte cultures. Effect of conA stimulation

Victor Bernard Hatcher, Allen J. Norin

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Trypsin-activated protease activity was observed in the supernatants of conA-treated unfractionated or monocyte-depleted cultures at 1 h of incubation and trypsin-inhibitory activity was detected at 24 h. In contrast, supernatants from wheat germ agglutinin (WGA)-treated cultures or untreated cultures exhibited trypsin-inhibitory activity at 1 h and trypsinactivated protease activity after 24 h. The expression of proteases and protease inhibitors may be early events that occur during the activation of quiescent lymphocytes to a proliferative state.

Original languageEnglish (US)
Pages (from-to)471-476
Number of pages6
JournalExperimental Cell Research
Volume142
Issue number2
DOIs
StatePublished - 1982

Fingerprint

Mononuclear Leukocytes
Human Activities
Trypsin
Peptide Hydrolases
Wheat Germ Agglutinins
Lymphocyte Activation
Protease Inhibitors
Monocytes

ASJC Scopus subject areas

  • Cell Biology

Cite this

@article{66ffff889872427e9f1ed0fd4ef84dd1,
title = "Expression of protease and protease-inhibitory activity in human mononuclear leukocyte cultures. Effect of conA stimulation",
abstract = "Trypsin-activated protease activity was observed in the supernatants of conA-treated unfractionated or monocyte-depleted cultures at 1 h of incubation and trypsin-inhibitory activity was detected at 24 h. In contrast, supernatants from wheat germ agglutinin (WGA)-treated cultures or untreated cultures exhibited trypsin-inhibitory activity at 1 h and trypsinactivated protease activity after 24 h. The expression of proteases and protease inhibitors may be early events that occur during the activation of quiescent lymphocytes to a proliferative state.",
author = "Hatcher, {Victor Bernard} and Norin, {Allen J.}",
year = "1982",
doi = "10.1016/0014-4827(82)90392-5",
language = "English (US)",
volume = "142",
pages = "471--476",
journal = "Experimental Cell Research",
issn = "0014-4827",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - Expression of protease and protease-inhibitory activity in human mononuclear leukocyte cultures. Effect of conA stimulation

AU - Hatcher, Victor Bernard

AU - Norin, Allen J.

PY - 1982

Y1 - 1982

N2 - Trypsin-activated protease activity was observed in the supernatants of conA-treated unfractionated or monocyte-depleted cultures at 1 h of incubation and trypsin-inhibitory activity was detected at 24 h. In contrast, supernatants from wheat germ agglutinin (WGA)-treated cultures or untreated cultures exhibited trypsin-inhibitory activity at 1 h and trypsinactivated protease activity after 24 h. The expression of proteases and protease inhibitors may be early events that occur during the activation of quiescent lymphocytes to a proliferative state.

AB - Trypsin-activated protease activity was observed in the supernatants of conA-treated unfractionated or monocyte-depleted cultures at 1 h of incubation and trypsin-inhibitory activity was detected at 24 h. In contrast, supernatants from wheat germ agglutinin (WGA)-treated cultures or untreated cultures exhibited trypsin-inhibitory activity at 1 h and trypsinactivated protease activity after 24 h. The expression of proteases and protease inhibitors may be early events that occur during the activation of quiescent lymphocytes to a proliferative state.

UR - http://www.scopus.com/inward/record.url?scp=0020434458&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0020434458&partnerID=8YFLogxK

U2 - 10.1016/0014-4827(82)90392-5

DO - 10.1016/0014-4827(82)90392-5

M3 - Article

C2 - 6756946

AN - SCOPUS:0020434458

VL - 142

SP - 471

EP - 476

JO - Experimental Cell Research

JF - Experimental Cell Research

SN - 0014-4827

IS - 2

ER -