Expression of prostaglandin G H synthase (cyclooxygenase) during murine fetal thymic development

Pierette M. Appasamy, Kimberly Pendino, Richard R. Schmidt, Kenneth P. Chepenik, Michael B. Prystowsky, Dan Goldowitz

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Fetal thymic lobes in organ culture have been shown to have the capacity to metabolize [14C]arachidonic acid (AA) to prostaglandins (PGs), including 6-ketoPGF, PGF, PGE2, and PGA2. Inhibition of AA metabolism results in inhibition of growth and Thy 1 expression during thymic organ culture. We report herein that freshly-isolated fetal thymic lobes also have the capacity to metabolize [14C]AA to PGs and HETEs at Days 14 and 16 of prenatal murine development. RNA encoding phospholipase A2, which liberates arachidonic acid from membrane phospholipids, and Cyclooxygenase (prostaglandin G H synthase), the first enzyme involved in the conversion of AA to PGs, are expressed during thymic development. We have localized the cyclooxygenase protein to stromal cells in the fetal and adult thymus. Exogenous AA or an analogue of PGI2 (iloprost) stimulated growth of fetal thymocytes in organ culture. These findings, together with our studies of the morphology of thymic lobes cultured with inhibitors of arachidonate metabolism, support the hypothesis that PGs are required for thymocyte proliferation during thymic development.

Original languageEnglish (US)
Pages (from-to)341-357
Number of pages17
JournalCellular Immunology
Volume137
Issue number2
DOIs
StatePublished - Oct 15 1991
Externally publishedYes

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Prostaglandin-Endoperoxide Synthases
Fetal Development
Arachidonic Acid
Prostaglandins
Organ Culture Techniques
Thymocytes
Iloprost
Hydroxyeicosatetraenoic Acids
Dinoprost
Phospholipases A2
Epoprostenol
Stromal Cells
Dinoprostone
Thymus Gland
Phospholipids
RNA
Membranes
Enzymes
Growth
Proteins

ASJC Scopus subject areas

  • Cell Biology
  • Immunology

Cite this

Expression of prostaglandin G H synthase (cyclooxygenase) during murine fetal thymic development. / Appasamy, Pierette M.; Pendino, Kimberly; Schmidt, Richard R.; Chepenik, Kenneth P.; Prystowsky, Michael B.; Goldowitz, Dan.

In: Cellular Immunology, Vol. 137, No. 2, 15.10.1991, p. 341-357.

Research output: Contribution to journalArticle

Appasamy, Pierette M. ; Pendino, Kimberly ; Schmidt, Richard R. ; Chepenik, Kenneth P. ; Prystowsky, Michael B. ; Goldowitz, Dan. / Expression of prostaglandin G H synthase (cyclooxygenase) during murine fetal thymic development. In: Cellular Immunology. 1991 ; Vol. 137, No. 2. pp. 341-357.
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