Expression of Panulirus shaker potassium channel splice variants

Marshall Kim, Deborah J. Baro, Cathy C. Lanning, Mehul Doshi, Howard S. Moskowitz, Jeremy Farnham, Ronald M. Harris-Warrick

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

In Drosophila shaker voltage-dependent potassium channels, alternative splicing at the amino and carboxy termini produces currents with different electrophysiological characteristics. We have cloned alternatively spliced forms of shaker from the spiny lobster Panulirus interruptus. Alternative exons were found at three sites of the gene: eight different 5' exons, two alternative exons encoding the pore-forming P region, and an alternative 3' exon. Two of the different amino terminal splice forms were expressed with two alternatively spliced pore forms to produce channels with markedly different characteristics. One of the amino termini produced a channel with transient characteristics while the other produced a delayed rectifier-type channel. The effects of alternative exons at the amino terminus and in the P region appear to be additive. Our results provide new information on the structural requirements for rapid N-type inactivation.

Original languageEnglish (US)
Pages (from-to)291-304
Number of pages14
JournalReceptors and Channels
Volume5
Issue number5
StatePublished - 1998
Externally publishedYes

Fingerprint

Shaker Superfamily of Potassium Channels
Palinuridae
Exons
Potassium Channels
Alternative Splicing
Drosophila
Genes
Electric potential

Keywords

  • Alternative splicing
  • Inactivation
  • Panulirus
  • Potassium channel
  • Shaker

ASJC Scopus subject areas

  • Pharmacology
  • Clinical Biochemistry
  • Cell Biology

Cite this

Kim, M., Baro, D. J., Lanning, C. C., Doshi, M., Moskowitz, H. S., Farnham, J., & Harris-Warrick, R. M. (1998). Expression of Panulirus shaker potassium channel splice variants. Receptors and Channels, 5(5), 291-304.

Expression of Panulirus shaker potassium channel splice variants. / Kim, Marshall; Baro, Deborah J.; Lanning, Cathy C.; Doshi, Mehul; Moskowitz, Howard S.; Farnham, Jeremy; Harris-Warrick, Ronald M.

In: Receptors and Channels, Vol. 5, No. 5, 1998, p. 291-304.

Research output: Contribution to journalArticle

Kim, M, Baro, DJ, Lanning, CC, Doshi, M, Moskowitz, HS, Farnham, J & Harris-Warrick, RM 1998, 'Expression of Panulirus shaker potassium channel splice variants', Receptors and Channels, vol. 5, no. 5, pp. 291-304.
Kim M, Baro DJ, Lanning CC, Doshi M, Moskowitz HS, Farnham J et al. Expression of Panulirus shaker potassium channel splice variants. Receptors and Channels. 1998;5(5):291-304.
Kim, Marshall ; Baro, Deborah J. ; Lanning, Cathy C. ; Doshi, Mehul ; Moskowitz, Howard S. ; Farnham, Jeremy ; Harris-Warrick, Ronald M. / Expression of Panulirus shaker potassium channel splice variants. In: Receptors and Channels. 1998 ; Vol. 5, No. 5. pp. 291-304.
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