Expression of multiple insecticidal genes confers broad resistance against a range of different rice pests

Shahina B. Maqbool, Sheikh Riazuddin, Nguyen Thi Loc, Angharad M R Gatehouse, John A. Gatehouse, Paul Christou

Research output: Contribution to journalArticle

168 Citations (Scopus)

Abstract

We report the simultaneous introduction of three insecticidal genes (the Bt genes cry1Ac and cry2A, and the snowdrop lectin gene gna) into commercially important indica rice varieties M7 and Basmati 370, by particle bombardment. Transgenic plants expressed Cry1Ac, Cry2A and GNA at different levels, either singly or in combination at 0.03-1%, 0.01-0.5% and 0.01-2.5% of total soluble protein, respectively. The transgenes showed stable transmission and expression, and R1 transgenic plants provided significant (p<0.01) protection against three of the most important insect pests of rice: rice leaf folder (Cnaphalocrocis medinalis), yellow stemborer (Scirpophaga incertulas) and brown planthopper (Nilaparvata lugens). The triple transformants showed significantly (p<0.05) higher resistance to these insects than plants expressing single transgenes. Bioassays using the triple-transgenic plants showed 100% eradication of the rice leaf folder and yellow stem borer, and 25% reduction in the survival of the brown planthopper. The greatest reduction in insect survival, and the greatest reduction in plant damage, occurred in plants expressing all three transgenes. This approach maximises the utility of gene transfer technology to introduce combinations of genes whose products disrupt different biochemical or physiological processes in the same insect, providing a multi-mechanism defence.

Original languageEnglish (US)
Pages (from-to)85-93
Number of pages9
JournalMolecular Breeding
Volume7
Issue number1
DOIs
StatePublished - 2001
Externally publishedYes

Fingerprint

Cnaphalocrocis medinalis
Nilaparvata lugens
Genes
Scirpophaga incertulas
pests
Insects
transgenes
Genetically Modified Plants
rice
transgenic plants
Transgenes
insects
genes
Gene transfer
Biochemical Phenomena
Bioassay
Physiological Phenomena
plant damage
Technology Transfer
defense mechanisms

Keywords

  • Brown planthopper
  • Cry1Ac
  • Cry2A
  • GNA
  • Insect resistance
  • Rice leaf folder
  • Yellow stem borer

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Horticulture
  • Biotechnology
  • Plant Science
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry

Cite this

Expression of multiple insecticidal genes confers broad resistance against a range of different rice pests. / Maqbool, Shahina B.; Riazuddin, Sheikh; Loc, Nguyen Thi; Gatehouse, Angharad M R; Gatehouse, John A.; Christou, Paul.

In: Molecular Breeding, Vol. 7, No. 1, 2001, p. 85-93.

Research output: Contribution to journalArticle

Maqbool, Shahina B. ; Riazuddin, Sheikh ; Loc, Nguyen Thi ; Gatehouse, Angharad M R ; Gatehouse, John A. ; Christou, Paul. / Expression of multiple insecticidal genes confers broad resistance against a range of different rice pests. In: Molecular Breeding. 2001 ; Vol. 7, No. 1. pp. 85-93.
@article{df1f48ecac55428bb0f9cd78999ef474,
title = "Expression of multiple insecticidal genes confers broad resistance against a range of different rice pests",
abstract = "We report the simultaneous introduction of three insecticidal genes (the Bt genes cry1Ac and cry2A, and the snowdrop lectin gene gna) into commercially important indica rice varieties M7 and Basmati 370, by particle bombardment. Transgenic plants expressed Cry1Ac, Cry2A and GNA at different levels, either singly or in combination at 0.03-1{\%}, 0.01-0.5{\%} and 0.01-2.5{\%} of total soluble protein, respectively. The transgenes showed stable transmission and expression, and R1 transgenic plants provided significant (p<0.01) protection against three of the most important insect pests of rice: rice leaf folder (Cnaphalocrocis medinalis), yellow stemborer (Scirpophaga incertulas) and brown planthopper (Nilaparvata lugens). The triple transformants showed significantly (p<0.05) higher resistance to these insects than plants expressing single transgenes. Bioassays using the triple-transgenic plants showed 100{\%} eradication of the rice leaf folder and yellow stem borer, and 25{\%} reduction in the survival of the brown planthopper. The greatest reduction in insect survival, and the greatest reduction in plant damage, occurred in plants expressing all three transgenes. This approach maximises the utility of gene transfer technology to introduce combinations of genes whose products disrupt different biochemical or physiological processes in the same insect, providing a multi-mechanism defence.",
keywords = "Brown planthopper, Cry1Ac, Cry2A, GNA, Insect resistance, Rice leaf folder, Yellow stem borer",
author = "Maqbool, {Shahina B.} and Sheikh Riazuddin and Loc, {Nguyen Thi} and Gatehouse, {Angharad M R} and Gatehouse, {John A.} and Paul Christou",
year = "2001",
doi = "10.1023/A:1009644712157",
language = "English (US)",
volume = "7",
pages = "85--93",
journal = "Molecular Breeding",
issn = "1380-3743",
publisher = "Springer Netherlands",
number = "1",

}

TY - JOUR

T1 - Expression of multiple insecticidal genes confers broad resistance against a range of different rice pests

AU - Maqbool, Shahina B.

AU - Riazuddin, Sheikh

AU - Loc, Nguyen Thi

AU - Gatehouse, Angharad M R

AU - Gatehouse, John A.

AU - Christou, Paul

PY - 2001

Y1 - 2001

N2 - We report the simultaneous introduction of three insecticidal genes (the Bt genes cry1Ac and cry2A, and the snowdrop lectin gene gna) into commercially important indica rice varieties M7 and Basmati 370, by particle bombardment. Transgenic plants expressed Cry1Ac, Cry2A and GNA at different levels, either singly or in combination at 0.03-1%, 0.01-0.5% and 0.01-2.5% of total soluble protein, respectively. The transgenes showed stable transmission and expression, and R1 transgenic plants provided significant (p<0.01) protection against three of the most important insect pests of rice: rice leaf folder (Cnaphalocrocis medinalis), yellow stemborer (Scirpophaga incertulas) and brown planthopper (Nilaparvata lugens). The triple transformants showed significantly (p<0.05) higher resistance to these insects than plants expressing single transgenes. Bioassays using the triple-transgenic plants showed 100% eradication of the rice leaf folder and yellow stem borer, and 25% reduction in the survival of the brown planthopper. The greatest reduction in insect survival, and the greatest reduction in plant damage, occurred in plants expressing all three transgenes. This approach maximises the utility of gene transfer technology to introduce combinations of genes whose products disrupt different biochemical or physiological processes in the same insect, providing a multi-mechanism defence.

AB - We report the simultaneous introduction of three insecticidal genes (the Bt genes cry1Ac and cry2A, and the snowdrop lectin gene gna) into commercially important indica rice varieties M7 and Basmati 370, by particle bombardment. Transgenic plants expressed Cry1Ac, Cry2A and GNA at different levels, either singly or in combination at 0.03-1%, 0.01-0.5% and 0.01-2.5% of total soluble protein, respectively. The transgenes showed stable transmission and expression, and R1 transgenic plants provided significant (p<0.01) protection against three of the most important insect pests of rice: rice leaf folder (Cnaphalocrocis medinalis), yellow stemborer (Scirpophaga incertulas) and brown planthopper (Nilaparvata lugens). The triple transformants showed significantly (p<0.05) higher resistance to these insects than plants expressing single transgenes. Bioassays using the triple-transgenic plants showed 100% eradication of the rice leaf folder and yellow stem borer, and 25% reduction in the survival of the brown planthopper. The greatest reduction in insect survival, and the greatest reduction in plant damage, occurred in plants expressing all three transgenes. This approach maximises the utility of gene transfer technology to introduce combinations of genes whose products disrupt different biochemical or physiological processes in the same insect, providing a multi-mechanism defence.

KW - Brown planthopper

KW - Cry1Ac

KW - Cry2A

KW - GNA

KW - Insect resistance

KW - Rice leaf folder

KW - Yellow stem borer

UR - http://www.scopus.com/inward/record.url?scp=0035094826&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035094826&partnerID=8YFLogxK

U2 - 10.1023/A:1009644712157

DO - 10.1023/A:1009644712157

M3 - Article

AN - SCOPUS:0035094826

VL - 7

SP - 85

EP - 93

JO - Molecular Breeding

JF - Molecular Breeding

SN - 1380-3743

IS - 1

ER -