Expression of mitochondrial cytochrome c oxidase in human colonic cell differentiation, transformation, and risk for colonic cancer

Barbara G. Heerdt, Heidi K. Halsey, Martin Lipkin, Leonard H. Augenlicht

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74 Citations (Scopus)

Abstract

In a panel of eight cloned complementary DNA sequences whose level of expression characterize colon cells as transformed in vivo and in vitro, one which may also serve as a marker of risk in familial polyposis and familial colon cancer flat mucosa has been identified as mitochondrial cytochrome c oxidase subunit 3. Mean level of expression of cytochrome c oxidase subunit 3 decreases progressively in colon adenomas and carcinomas relative to normal mucosa in vivo, and returns to higher levels present in biopsies of normal mucosa when the HT29 human colonic adenocarcinoma cell line is induced to differentiate with sodium butyrate. Quantitation of cytochrome c oxidase subunit 3 DNA by dot blots indicated that these changes in expression were not associated with alterations in the number of mitochondrial genomes.

Original languageEnglish (US)
Pages (from-to)1596-1600
Number of pages5
JournalCancer Research
Volume50
Issue number5
StatePublished - Mar 1 1990

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Electron Transport Complex IV
Colonic Neoplasms
Cell Differentiation
Mucous Membrane
Colon
Mitochondrial Genome
Butyric Acid
Adenoma
Adenocarcinoma
Complementary DNA
Carcinoma
Biopsy
Cell Line
DNA

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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Expression of mitochondrial cytochrome c oxidase in human colonic cell differentiation, transformation, and risk for colonic cancer. / Heerdt, Barbara G.; Halsey, Heidi K.; Lipkin, Martin; Augenlicht, Leonard H.

In: Cancer Research, Vol. 50, No. 5, 01.03.1990, p. 1596-1600.

Research output: Contribution to journalArticle

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