TY - JOUR
T1 - Expression of microtubule-associated protein-2a and other novel microtubule-associated protein-2 transcripts in human fetal spinal cord
AU - Kalcheva, Nellie
AU - Weidenheim, Karen M.
AU - Kress, Yvonne
AU - Shafit-Zagardo, Bridget
PY - 1997/1
Y1 - 1997/1
N2 - In human fetal spinal cord (HFSC), six additional microtubule- associated protein-2 (MAP-2) transcripts are generated by alternative splicing of two recently described exons, exon 8 and exon 13. The following three translated proteins are detected by western blot analysis: MAP-2b expressing exon 8 (MAP-2b+8; MAP-2a), MAP-2b expressing exon 13 (MAP-2b+13), and MAP-2c expressing exon 8 and exon 13 (MAP-2c+8+13). The finding that MAP- 2b+8 is expressed in HFSC demonstrates for the first time the presence of MAP-2a in human fetal CNS. Immunocytochemical studies show that exon 8- specific antibody and exon 13-specific antibody stain independent and overlapping populations of neurons in the lumbar region of the HFSC. Antibody 13-immunopositive neurons have predominantly cytosolic staining, whereas in the antibody 8-immunoreactive neurons staining was observed in the cytosol, dendrites, and some synapses. The prenatal expression of MAP-2a, which has been used as a marker of synaptogenesis, not only demonstrates the presence of a mature MAP-2 isoform in HFSC, but suggests that MAP-2a is important during human fetal as well as post-natal synaptogenesis.
AB - In human fetal spinal cord (HFSC), six additional microtubule- associated protein-2 (MAP-2) transcripts are generated by alternative splicing of two recently described exons, exon 8 and exon 13. The following three translated proteins are detected by western blot analysis: MAP-2b expressing exon 8 (MAP-2b+8; MAP-2a), MAP-2b expressing exon 13 (MAP-2b+13), and MAP-2c expressing exon 8 and exon 13 (MAP-2c+8+13). The finding that MAP- 2b+8 is expressed in HFSC demonstrates for the first time the presence of MAP-2a in human fetal CNS. Immunocytochemical studies show that exon 8- specific antibody and exon 13-specific antibody stain independent and overlapping populations of neurons in the lumbar region of the HFSC. Antibody 13-immunopositive neurons have predominantly cytosolic staining, whereas in the antibody 8-immunoreactive neurons staining was observed in the cytosol, dendrites, and some synapses. The prenatal expression of MAP-2a, which has been used as a marker of synaptogenesis, not only demonstrates the presence of a mature MAP-2 isoform in HFSC, but suggests that MAP-2a is important during human fetal as well as post-natal synaptogenesis.
KW - Development
KW - Human fetal spinal cord
KW - Microtubule-associated protein- 2b+8
KW - Microtubule-associated protein-2a
KW - Synaptogenesis
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U2 - 10.1046/j.1471-4159.1997.68010383.x
DO - 10.1046/j.1471-4159.1997.68010383.x
M3 - Article
C2 - 8978750
AN - SCOPUS:0031037198
SN - 0022-3042
VL - 68
SP - 383
EP - 391
JO - Journal of Neurochemistry
JF - Journal of Neurochemistry
IS - 1
ER -