Expression of metalloproteinases and inhibitors in the differentiation of P19CL6 cells into cardiac myocytes

David A. Young, Svetlana Gavrilov, Caroline J. Pennington, Robert K. Nuttall, Dylan R. Edwards, Richard N. Kitsis, Ian M. Clark

Research output: Contribution to journalArticle

30 Scopus citations

Abstract

P19CL6 are a clonal derivative of P19 embryonal carcinoma cells, a euploid, multipotent mouse cell line, that differentiate efficiently into cardiac myocytes, with spontaneous beating evident within 10 days, following DMSO treatment. Using real-time quantitative RT-PCR we have profiled the expression of the complete matrix metalloproteinase and tissue inhibitor of metalloproteinase gene families during P19CL6 differentiation to cardiac myocytes. The genes subdivide into eight groups based upon their expression profile. Their expression was both qualitatively and quantitatively highly homologous to that seen during mouse heart development.

Original languageEnglish (US)
Pages (from-to)759-765
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume322
Issue number3
DOIs
StatePublished - Sep 24 2004

Keywords

  • Cardiac myocyte
  • Expression
  • MMP
  • TIMP

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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