Expression of metalloproteinases and inhibitors in the differentiation of P19CL6 cells into cardiac myocytes

David A. Young; Svetlana Gavrilov; Caroline J. Pennington; Robert K. Nuttall; Dylan R. Edwards; Richard N. Kitsis; Ian M. Clark

doi:10.1016/j.bbrc.2004.07.178

Expression of metalloproteinases and inhibitors in the differentiation of P19CL6 cells into cardiac myocytes

David A. Young, Svetlana Gavrilov, Caroline J. Pennington, Robert K. Nuttall, Dylan R. Edwards, Richard N. Kitsis, Ian M. Clark

Medicine

Research output: Contribution to journal › Article › peer-review

42 Scopus citations

Abstract

P19CL6 are a clonal derivative of P19 embryonal carcinoma cells, a euploid, multipotent mouse cell line, that differentiate efficiently into cardiac myocytes, with spontaneous beating evident within 10 days, following DMSO treatment. Using real-time quantitative RT-PCR we have profiled the expression of the complete matrix metalloproteinase and tissue inhibitor of metalloproteinase gene families during P19CL6 differentiation to cardiac myocytes. The genes subdivide into eight groups based upon their expression profile. Their expression was both qualitatively and quantitatively highly homologous to that seen during mouse heart development.

Original language	English (US)
Pages (from-to)	759-765
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	322
Issue number	3
DOIs	https://doi.org/10.1016/j.bbrc.2004.07.178
State	Published - Sep 24 2004

Keywords

Cardiac myocyte
Expression
MMP
TIMP

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/j.bbrc.2004.07.178

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title = "Expression of metalloproteinases and inhibitors in the differentiation of P19CL6 cells into cardiac myocytes",

abstract = "P19CL6 are a clonal derivative of P19 embryonal carcinoma cells, a euploid, multipotent mouse cell line, that differentiate efficiently into cardiac myocytes, with spontaneous beating evident within 10 days, following DMSO treatment. Using real-time quantitative RT-PCR we have profiled the expression of the complete matrix metalloproteinase and tissue inhibitor of metalloproteinase gene families during P19CL6 differentiation to cardiac myocytes. The genes subdivide into eight groups based upon their expression profile. Their expression was both qualitatively and quantitatively highly homologous to that seen during mouse heart development.",

keywords = "Cardiac myocyte, Expression, MMP, TIMP",

author = "Young, {David A.} and Svetlana Gavrilov and Pennington, {Caroline J.} and Nuttall, {Robert K.} and Edwards, {Dylan R.} and Kitsis, {Richard N.} and Clark, {Ian M.}",

note = "Funding Information: David A. Young wishes to acknowledge the support of the Dunhill Medical Trust, UK. This work was supported in part by grants to Richard N. Kitsis from the National Institutes of Health (R01 HL60665 and R01 HL61550) and the Monique Weill-Caulier Scholar Award. Dr. Kitsis is the Dr. Gerald and Myra Dorros Chair in Cardiovascular Disease of the Albert Einstein College of Medicine.",

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AU - Young, David A.

AU - Gavrilov, Svetlana

AU - Pennington, Caroline J.

AU - Nuttall, Robert K.

AU - Edwards, Dylan R.

AU - Kitsis, Richard N.

AU - Clark, Ian M.

N1 - Funding Information: David A. Young wishes to acknowledge the support of the Dunhill Medical Trust, UK. This work was supported in part by grants to Richard N. Kitsis from the National Institutes of Health (R01 HL60665 and R01 HL61550) and the Monique Weill-Caulier Scholar Award. Dr. Kitsis is the Dr. Gerald and Myra Dorros Chair in Cardiovascular Disease of the Albert Einstein College of Medicine.

PY - 2004/9/24

Y1 - 2004/9/24

N2 - P19CL6 are a clonal derivative of P19 embryonal carcinoma cells, a euploid, multipotent mouse cell line, that differentiate efficiently into cardiac myocytes, with spontaneous beating evident within 10 days, following DMSO treatment. Using real-time quantitative RT-PCR we have profiled the expression of the complete matrix metalloproteinase and tissue inhibitor of metalloproteinase gene families during P19CL6 differentiation to cardiac myocytes. The genes subdivide into eight groups based upon their expression profile. Their expression was both qualitatively and quantitatively highly homologous to that seen during mouse heart development.

AB - P19CL6 are a clonal derivative of P19 embryonal carcinoma cells, a euploid, multipotent mouse cell line, that differentiate efficiently into cardiac myocytes, with spontaneous beating evident within 10 days, following DMSO treatment. Using real-time quantitative RT-PCR we have profiled the expression of the complete matrix metalloproteinase and tissue inhibitor of metalloproteinase gene families during P19CL6 differentiation to cardiac myocytes. The genes subdivide into eight groups based upon their expression profile. Their expression was both qualitatively and quantitatively highly homologous to that seen during mouse heart development.

KW - Cardiac myocyte

KW - Expression

KW - MMP

KW - TIMP

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Expression of metalloproteinases and inhibitors in the differentiation of P19CL6 cells into cardiac myocytes

Abstract

Keywords

ASJC Scopus subject areas

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