Expression of metalloproteinases and inhibitors in the differentiation of P19CL6 cells into cardiac myocytes

David A. Young, Svetlana Gavrilov, Caroline J. Pennington, Robert K. Nuttall, Dylan R. Edwards, Richard N. Kitsis, Ian M. Clark

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

P19CL6 are a clonal derivative of P19 embryonal carcinoma cells, a euploid, multipotent mouse cell line, that differentiate efficiently into cardiac myocytes, with spontaneous beating evident within 10 days, following DMSO treatment. Using real-time quantitative RT-PCR we have profiled the expression of the complete matrix metalloproteinase and tissue inhibitor of metalloproteinase gene families during P19CL6 differentiation to cardiac myocytes. The genes subdivide into eight groups based upon their expression profile. Their expression was both qualitatively and quantitatively highly homologous to that seen during mouse heart development.

Original languageEnglish (US)
Pages (from-to)759-765
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume322
Issue number3
DOIs
StatePublished - Sep 24 2004

Fingerprint

Metalloproteases
Cardiac Myocytes
Cell Differentiation
Genes
Cells
Embryonal Carcinoma Stem Cells
Tissue Inhibitor of Metalloproteinases
Matrix Metalloproteinase Inhibitors
Dimethyl Sulfoxide
Matrix Metalloproteinases
Real-Time Polymerase Chain Reaction
Derivatives
Cell Line

Keywords

  • Cardiac myocyte
  • Expression
  • MMP
  • TIMP

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Expression of metalloproteinases and inhibitors in the differentiation of P19CL6 cells into cardiac myocytes. / Young, David A.; Gavrilov, Svetlana; Pennington, Caroline J.; Nuttall, Robert K.; Edwards, Dylan R.; Kitsis, Richard N.; Clark, Ian M.

In: Biochemical and Biophysical Research Communications, Vol. 322, No. 3, 24.09.2004, p. 759-765.

Research output: Contribution to journalArticle

Young, David A. ; Gavrilov, Svetlana ; Pennington, Caroline J. ; Nuttall, Robert K. ; Edwards, Dylan R. ; Kitsis, Richard N. ; Clark, Ian M. / Expression of metalloproteinases and inhibitors in the differentiation of P19CL6 cells into cardiac myocytes. In: Biochemical and Biophysical Research Communications. 2004 ; Vol. 322, No. 3. pp. 759-765.
@article{a300f78af6354c19931f34117fceb6fd,
title = "Expression of metalloproteinases and inhibitors in the differentiation of P19CL6 cells into cardiac myocytes",
abstract = "P19CL6 are a clonal derivative of P19 embryonal carcinoma cells, a euploid, multipotent mouse cell line, that differentiate efficiently into cardiac myocytes, with spontaneous beating evident within 10 days, following DMSO treatment. Using real-time quantitative RT-PCR we have profiled the expression of the complete matrix metalloproteinase and tissue inhibitor of metalloproteinase gene families during P19CL6 differentiation to cardiac myocytes. The genes subdivide into eight groups based upon their expression profile. Their expression was both qualitatively and quantitatively highly homologous to that seen during mouse heart development.",
keywords = "Cardiac myocyte, Expression, MMP, TIMP",
author = "Young, {David A.} and Svetlana Gavrilov and Pennington, {Caroline J.} and Nuttall, {Robert K.} and Edwards, {Dylan R.} and Kitsis, {Richard N.} and Clark, {Ian M.}",
year = "2004",
month = "9",
day = "24",
doi = "10.1016/j.bbrc.2004.07.178",
language = "English (US)",
volume = "322",
pages = "759--765",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "3",

}

TY - JOUR

T1 - Expression of metalloproteinases and inhibitors in the differentiation of P19CL6 cells into cardiac myocytes

AU - Young, David A.

AU - Gavrilov, Svetlana

AU - Pennington, Caroline J.

AU - Nuttall, Robert K.

AU - Edwards, Dylan R.

AU - Kitsis, Richard N.

AU - Clark, Ian M.

PY - 2004/9/24

Y1 - 2004/9/24

N2 - P19CL6 are a clonal derivative of P19 embryonal carcinoma cells, a euploid, multipotent mouse cell line, that differentiate efficiently into cardiac myocytes, with spontaneous beating evident within 10 days, following DMSO treatment. Using real-time quantitative RT-PCR we have profiled the expression of the complete matrix metalloproteinase and tissue inhibitor of metalloproteinase gene families during P19CL6 differentiation to cardiac myocytes. The genes subdivide into eight groups based upon their expression profile. Their expression was both qualitatively and quantitatively highly homologous to that seen during mouse heart development.

AB - P19CL6 are a clonal derivative of P19 embryonal carcinoma cells, a euploid, multipotent mouse cell line, that differentiate efficiently into cardiac myocytes, with spontaneous beating evident within 10 days, following DMSO treatment. Using real-time quantitative RT-PCR we have profiled the expression of the complete matrix metalloproteinase and tissue inhibitor of metalloproteinase gene families during P19CL6 differentiation to cardiac myocytes. The genes subdivide into eight groups based upon their expression profile. Their expression was both qualitatively and quantitatively highly homologous to that seen during mouse heart development.

KW - Cardiac myocyte

KW - Expression

KW - MMP

KW - TIMP

UR - http://www.scopus.com/inward/record.url?scp=4444322829&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=4444322829&partnerID=8YFLogxK

U2 - 10.1016/j.bbrc.2004.07.178

DO - 10.1016/j.bbrc.2004.07.178

M3 - Article

VL - 322

SP - 759

EP - 765

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 3

ER -