Expression of ghrelin and biological activity of specific receptors for ghrelin and des-acyl ghrelin in human prostate neoplasms and related cell lines

Paola Cassoni, Corrado Ghé, Tiziana Marrocco, Elena Tarabra, Elena Allia, Filomena Catapano, Romano Deghenghi, Ezio Ghigo, Mauro Papotti, Giampiero Muccioli

Research output: Contribution to journalArticle

176 Citations (Scopus)

Abstract

Background: Ghrelin, a natural growth hormone secretagogue (GHS), has been identified in prostate carcinoma cell lines. Objectives: To investigate the presence of ghrelin and its receptors in human prostate tumours and in DU-145, PC-3 and LNCaP prostate carcinoma cell lines, and to assesss the effects of ghrelin and its more abundant circulating form, des-octanoyl ghrelin, on cell proliferation. Methods: Ghrelin and types 1a and 1b GHS receptor (GHS-R) were determined at the mRNA and protein levels by RT-PCR, in situ hybridization, immunohistochemistry and enzyme immunoassay in tissues, cell lines and culture medium. Ghrelin binding was determined by radioreceptor assay. The effects on cell proliferation were evaluated by growth curves. Results: Ghrelin mRNA was found in prostatic carcinomas and benign hyperplasias, but immunohistochemistry was negative. GHS-R1a and 1b mRNAs were absent from carcinomas, but GHS-R1b mRNA was present in 50% of hyperplasias. Ghrelin peptide and mRNA were present in PC-3 cells exclusively, whereas GHS-R1a and 1b mRNAs were expressed in DU-145 cells only. Specific [125I]Tyr4-ghrelin binding was detected in prostate tumour, DU-145 and PC-3 cell membranes and the binding was displaced by ghrelin, synthetic GHS and des-octanoyl ghrelin, which is devoid of GHS-R1a binding affinity and GH-releasing activity. Ghrelin and des-acyl ghrelin inhibited DU-145 cell proliferation, displayed a biphasic effect in PC-3 cells and were ineffective in LNCaP cells. Conclusions: Specific GHS binding sites, other than GHS-R1a and 1b, are present in human prostatic neoplasms. Ghrelin, in addition to des-acyl ghrelin, exerts different effects on cell proliferation in prostate carcinoma cell lines.

Original languageEnglish (US)
Pages (from-to)173-184
Number of pages12
JournalEuropean Journal of Endocrinology
Volume150
Issue number2
DOIs
StatePublished - Feb 2004
Externally publishedYes

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Ghrelin Receptor
Ghrelin
Prostatic Neoplasms
Cell Line
Growth Hormone
Prostate
Messenger RNA
Carcinoma
Cell Proliferation
des-n-octanoyl ghrelin
Immunohistochemistry
Radioligand Assay
Prostatic Hyperplasia
Immunoenzyme Techniques
Hyperplasia
In Situ Hybridization
Culture Media

ASJC Scopus subject areas

  • Endocrinology

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Expression of ghrelin and biological activity of specific receptors for ghrelin and des-acyl ghrelin in human prostate neoplasms and related cell lines. / Cassoni, Paola; Ghé, Corrado; Marrocco, Tiziana; Tarabra, Elena; Allia, Elena; Catapano, Filomena; Deghenghi, Romano; Ghigo, Ezio; Papotti, Mauro; Muccioli, Giampiero.

In: European Journal of Endocrinology, Vol. 150, No. 2, 02.2004, p. 173-184.

Research output: Contribution to journalArticle

Cassoni, P, Ghé, C, Marrocco, T, Tarabra, E, Allia, E, Catapano, F, Deghenghi, R, Ghigo, E, Papotti, M & Muccioli, G 2004, 'Expression of ghrelin and biological activity of specific receptors for ghrelin and des-acyl ghrelin in human prostate neoplasms and related cell lines', European Journal of Endocrinology, vol. 150, no. 2, pp. 173-184. https://doi.org/10.1530/eje.0.1500173
Cassoni, Paola ; Ghé, Corrado ; Marrocco, Tiziana ; Tarabra, Elena ; Allia, Elena ; Catapano, Filomena ; Deghenghi, Romano ; Ghigo, Ezio ; Papotti, Mauro ; Muccioli, Giampiero. / Expression of ghrelin and biological activity of specific receptors for ghrelin and des-acyl ghrelin in human prostate neoplasms and related cell lines. In: European Journal of Endocrinology. 2004 ; Vol. 150, No. 2. pp. 173-184.
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abstract = "Background: Ghrelin, a natural growth hormone secretagogue (GHS), has been identified in prostate carcinoma cell lines. Objectives: To investigate the presence of ghrelin and its receptors in human prostate tumours and in DU-145, PC-3 and LNCaP prostate carcinoma cell lines, and to assesss the effects of ghrelin and its more abundant circulating form, des-octanoyl ghrelin, on cell proliferation. Methods: Ghrelin and types 1a and 1b GHS receptor (GHS-R) were determined at the mRNA and protein levels by RT-PCR, in situ hybridization, immunohistochemistry and enzyme immunoassay in tissues, cell lines and culture medium. Ghrelin binding was determined by radioreceptor assay. The effects on cell proliferation were evaluated by growth curves. Results: Ghrelin mRNA was found in prostatic carcinomas and benign hyperplasias, but immunohistochemistry was negative. GHS-R1a and 1b mRNAs were absent from carcinomas, but GHS-R1b mRNA was present in 50{\%} of hyperplasias. Ghrelin peptide and mRNA were present in PC-3 cells exclusively, whereas GHS-R1a and 1b mRNAs were expressed in DU-145 cells only. Specific [125I]Tyr4-ghrelin binding was detected in prostate tumour, DU-145 and PC-3 cell membranes and the binding was displaced by ghrelin, synthetic GHS and des-octanoyl ghrelin, which is devoid of GHS-R1a binding affinity and GH-releasing activity. Ghrelin and des-acyl ghrelin inhibited DU-145 cell proliferation, displayed a biphasic effect in PC-3 cells and were ineffective in LNCaP cells. Conclusions: Specific GHS binding sites, other than GHS-R1a and 1b, are present in human prostatic neoplasms. Ghrelin, in addition to des-acyl ghrelin, exerts different effects on cell proliferation in prostate carcinoma cell lines.",
author = "Paola Cassoni and Corrado Gh{\'e} and Tiziana Marrocco and Elena Tarabra and Elena Allia and Filomena Catapano and Romano Deghenghi and Ezio Ghigo and Mauro Papotti and Giampiero Muccioli",
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T1 - Expression of ghrelin and biological activity of specific receptors for ghrelin and des-acyl ghrelin in human prostate neoplasms and related cell lines

AU - Cassoni, Paola

AU - Ghé, Corrado

AU - Marrocco, Tiziana

AU - Tarabra, Elena

AU - Allia, Elena

AU - Catapano, Filomena

AU - Deghenghi, Romano

AU - Ghigo, Ezio

AU - Papotti, Mauro

AU - Muccioli, Giampiero

PY - 2004/2

Y1 - 2004/2

N2 - Background: Ghrelin, a natural growth hormone secretagogue (GHS), has been identified in prostate carcinoma cell lines. Objectives: To investigate the presence of ghrelin and its receptors in human prostate tumours and in DU-145, PC-3 and LNCaP prostate carcinoma cell lines, and to assesss the effects of ghrelin and its more abundant circulating form, des-octanoyl ghrelin, on cell proliferation. Methods: Ghrelin and types 1a and 1b GHS receptor (GHS-R) were determined at the mRNA and protein levels by RT-PCR, in situ hybridization, immunohistochemistry and enzyme immunoassay in tissues, cell lines and culture medium. Ghrelin binding was determined by radioreceptor assay. The effects on cell proliferation were evaluated by growth curves. Results: Ghrelin mRNA was found in prostatic carcinomas and benign hyperplasias, but immunohistochemistry was negative. GHS-R1a and 1b mRNAs were absent from carcinomas, but GHS-R1b mRNA was present in 50% of hyperplasias. Ghrelin peptide and mRNA were present in PC-3 cells exclusively, whereas GHS-R1a and 1b mRNAs were expressed in DU-145 cells only. Specific [125I]Tyr4-ghrelin binding was detected in prostate tumour, DU-145 and PC-3 cell membranes and the binding was displaced by ghrelin, synthetic GHS and des-octanoyl ghrelin, which is devoid of GHS-R1a binding affinity and GH-releasing activity. Ghrelin and des-acyl ghrelin inhibited DU-145 cell proliferation, displayed a biphasic effect in PC-3 cells and were ineffective in LNCaP cells. Conclusions: Specific GHS binding sites, other than GHS-R1a and 1b, are present in human prostatic neoplasms. Ghrelin, in addition to des-acyl ghrelin, exerts different effects on cell proliferation in prostate carcinoma cell lines.

AB - Background: Ghrelin, a natural growth hormone secretagogue (GHS), has been identified in prostate carcinoma cell lines. Objectives: To investigate the presence of ghrelin and its receptors in human prostate tumours and in DU-145, PC-3 and LNCaP prostate carcinoma cell lines, and to assesss the effects of ghrelin and its more abundant circulating form, des-octanoyl ghrelin, on cell proliferation. Methods: Ghrelin and types 1a and 1b GHS receptor (GHS-R) were determined at the mRNA and protein levels by RT-PCR, in situ hybridization, immunohistochemistry and enzyme immunoassay in tissues, cell lines and culture medium. Ghrelin binding was determined by radioreceptor assay. The effects on cell proliferation were evaluated by growth curves. Results: Ghrelin mRNA was found in prostatic carcinomas and benign hyperplasias, but immunohistochemistry was negative. GHS-R1a and 1b mRNAs were absent from carcinomas, but GHS-R1b mRNA was present in 50% of hyperplasias. Ghrelin peptide and mRNA were present in PC-3 cells exclusively, whereas GHS-R1a and 1b mRNAs were expressed in DU-145 cells only. Specific [125I]Tyr4-ghrelin binding was detected in prostate tumour, DU-145 and PC-3 cell membranes and the binding was displaced by ghrelin, synthetic GHS and des-octanoyl ghrelin, which is devoid of GHS-R1a binding affinity and GH-releasing activity. Ghrelin and des-acyl ghrelin inhibited DU-145 cell proliferation, displayed a biphasic effect in PC-3 cells and were ineffective in LNCaP cells. Conclusions: Specific GHS binding sites, other than GHS-R1a and 1b, are present in human prostatic neoplasms. Ghrelin, in addition to des-acyl ghrelin, exerts different effects on cell proliferation in prostate carcinoma cell lines.

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