Expression of enzymatically active reverse transcriptase of simian immunodeficiency virus in bacteria

Sensitivity to nucleotide analogue inhibitors

Vinayaka R. Prasad, Kyl Myrick, William Haseltine, Stephen P. Goff

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

A fragment of the SIVmac251 pol gene was expressed in Escherichia coli as a trpE fusion protein. Analysis of extracts from bacteria containing this expression plasmid revealed the presence of a reverse transcriptase activity dependent on Mgt2+ as divalent cation and active on both poly(rA) · oligo(dT) and poly(rC · oligo(dG) templates. In comparative studies, the SIV and HIV-1 reverse transcriptases expressed in bacteria displayed very similar high sensitivities to the chain terminator inhibitors AZTTP and ddTTP. The reverse transcriptase of Moloney murine leukemia virus and the DNA polymerase of E. coli were both more resistant to ddTTP, and the E. coli enzyme was significantly more resistant to AZTTP.

Original languageEnglish (US)
Pages (from-to)896-900
Number of pages5
JournalVirology
Volume179
Issue number2
DOIs
StatePublished - 1990
Externally publishedYes

Fingerprint

Simian Immunodeficiency Virus
RNA-Directed DNA Polymerase
Nucleotides
Escherichia coli
Bacteria
pol Genes
Moloney murine leukemia virus
Divalent Cations
DNA-Directed DNA Polymerase
Plasmids
Enzymes
Proteins
2',3'-dideoxythymidine triphosphate
3'-azido-3'-deoxythymidine 5'-triphosphate

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Expression of enzymatically active reverse transcriptase of simian immunodeficiency virus in bacteria : Sensitivity to nucleotide analogue inhibitors. / Prasad, Vinayaka R.; Myrick, Kyl; Haseltine, William; Goff, Stephen P.

In: Virology, Vol. 179, No. 2, 1990, p. 896-900.

Research output: Contribution to journalArticle

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