Expression of cytochrome P45011B1 mRNA in the brain of normal and hypertensive transgenic rats

Bettina Erdmann, Hellmut Gerst, Andrea Lippoldt, Hannes Bülow, Detlev Ganten, Kjell Fuxe, Rita Bernhardt

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

Cytochrome P45011B1 (11β-hydroxylase) was detected in the brain of male rats by in situ hybridization methods. Normal Sprague-Dawley rats were compared to the transgenic strain TGR(mRen2)27, characterized by the expression of the murine Ren-2(d) renin gene and the development of severe hypertension. Specific riboprobes were generated by in vitro transcription of a 152 base-pair long cDNA template. 35S-labeled riboprobes were hybridized to cryostat sections from adrenal glands and from two different levels of the brain using standard protocols and varying washing conditions. After exposure of the radiolabeled sections to X-ray film, the signals were quantified and compared. Following autoradiography and counterstaining, cytochrome P45011B1 mRNA was clearly localized in the zona fasciculata/reticularis of the adrenal cortex and in distinct layers of the cerebral cortex. High signal densities were obtained in the layers II-IV of the neocortex and in the layer II of the piriform cortex, although the concentrations of cytochrome P45011B1 mRNA were remarkably lower in the central nervous system as compared to adrenal glands. As revealed by the semi-quantitative analysis, there was a slight increase in adrenal 11β-hydroxylase mRNA in the transgenic rats,whereas the brain seems to express nearly the same amount of this enzyme in both strains. The cytochrome P45011B1 mRNA expression in distinct cells, probably nerve cells, and especially in regions with high densities of glucocorticoid receptors points to a possible function of brain derived corticosterone in receptor activation.

Original languageEnglish (US)
Pages (from-to)73-82
Number of pages10
JournalBrain Research
Volume733
Issue number1
DOIs
StatePublished - Sep 9 1996
Externally publishedYes

Keywords

  • Cerebral cortex
  • Cytochrome P45011B1
  • Hypertension
  • In situ hybridization
  • Neurosteroid
  • Transgenic rat

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

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