Expression of CSF-1, c-fms, and MCP-1 in the central nervous system of rats with experimental allergie encephalomyelitis

Kathy Hulkower, Celia F. Brosnan, Dennis A. Aquino, Wendy Cammer, Sunita Kulshrestha, Michelle P. Guida, Davida A. Rapoport, Joan W. Berman

Research output: Contribution to journalArticle

195 Citations (Scopus)

Abstract

We have examined the expression of factors associated with the growth, differentiation, and chemotaxis of cells of the monocyte/macrophage series in the central nervous system of Lewis rats sensitized to develop experimental allergic encephalomyelitis. CSF-1 mRNA increased significantly over that found in control animals (sensitized with OVA in CFA or CFA alone). The elevation in the levels of this growth factor commenced immediately before the onset of early clinical signs and peaked immediately before maximal clinical incidence of disease. Expression of CSF-1 message declined to base-line values with resolution of the disease process. CSF-1 protein was also detected in the central nervous system at the height of clinical disease. Expression of the receptor for CSF-1, the proto-oncogene c-fms, also paralleled the early disease process. Elevated levels of c-fms mRNA were detected immediately before the onset and peaked at the height of clinical signs of disease. In contrast to CSF-1 levels, elevated c-fms message expression persisted after resolution of the acute phase of experimental allergic encephalomyelitis. Levels of macrophage chemotactic factor-1 message were also elevated immediately before the onset of clinical signs, peaked with the height of clinical disease, and declined with resolution of the disease. Unlike CSF-1 or c-fms, no endogenous macrophage chemotactic factor-1 message was detected in control animals. Macrophage chemotactic factor-1 protein was demonstrated by Western blot in the central nervous system at the height of clinical disease. The results support the conclusion that expression of factors that specifically target cells of the monocyte/ macrophage series are an important component of the disease process in experimental allergic encephalomyelitis.

Original languageEnglish (US)
Pages (from-to)2525-2533
Number of pages9
JournalJournal of Immunology
Volume150
Issue number6
StatePublished - 1993

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Encephalomyelitis
Macrophage Colony-Stimulating Factor
Central Nervous System
Autoimmune Experimental Encephalomyelitis
Chemotactic Factors
Monocytes
fms Genes
Macrophages
Macrophage Colony-Stimulating Factor Receptors
Messenger RNA
Chemotaxis
Cell Differentiation
Intercellular Signaling Peptides and Proteins
Proteins
Western Blotting

ASJC Scopus subject areas

  • Immunology

Cite this

Hulkower, K., Brosnan, C. F., Aquino, D. A., Cammer, W., Kulshrestha, S., Guida, M. P., ... Berman, J. W. (1993). Expression of CSF-1, c-fms, and MCP-1 in the central nervous system of rats with experimental allergie encephalomyelitis. Journal of Immunology, 150(6), 2525-2533.

Expression of CSF-1, c-fms, and MCP-1 in the central nervous system of rats with experimental allergie encephalomyelitis. / Hulkower, Kathy; Brosnan, Celia F.; Aquino, Dennis A.; Cammer, Wendy; Kulshrestha, Sunita; Guida, Michelle P.; Rapoport, Davida A.; Berman, Joan W.

In: Journal of Immunology, Vol. 150, No. 6, 1993, p. 2525-2533.

Research output: Contribution to journalArticle

Hulkower, K, Brosnan, CF, Aquino, DA, Cammer, W, Kulshrestha, S, Guida, MP, Rapoport, DA & Berman, JW 1993, 'Expression of CSF-1, c-fms, and MCP-1 in the central nervous system of rats with experimental allergie encephalomyelitis', Journal of Immunology, vol. 150, no. 6, pp. 2525-2533.
Hulkower K, Brosnan CF, Aquino DA, Cammer W, Kulshrestha S, Guida MP et al. Expression of CSF-1, c-fms, and MCP-1 in the central nervous system of rats with experimental allergie encephalomyelitis. Journal of Immunology. 1993;150(6):2525-2533.
Hulkower, Kathy ; Brosnan, Celia F. ; Aquino, Dennis A. ; Cammer, Wendy ; Kulshrestha, Sunita ; Guida, Michelle P. ; Rapoport, Davida A. ; Berman, Joan W. / Expression of CSF-1, c-fms, and MCP-1 in the central nervous system of rats with experimental allergie encephalomyelitis. In: Journal of Immunology. 1993 ; Vol. 150, No. 6. pp. 2525-2533.
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abstract = "We have examined the expression of factors associated with the growth, differentiation, and chemotaxis of cells of the monocyte/macrophage series in the central nervous system of Lewis rats sensitized to develop experimental allergic encephalomyelitis. CSF-1 mRNA increased significantly over that found in control animals (sensitized with OVA in CFA or CFA alone). The elevation in the levels of this growth factor commenced immediately before the onset of early clinical signs and peaked immediately before maximal clinical incidence of disease. Expression of CSF-1 message declined to base-line values with resolution of the disease process. CSF-1 protein was also detected in the central nervous system at the height of clinical disease. Expression of the receptor for CSF-1, the proto-oncogene c-fms, also paralleled the early disease process. Elevated levels of c-fms mRNA were detected immediately before the onset and peaked at the height of clinical signs of disease. In contrast to CSF-1 levels, elevated c-fms message expression persisted after resolution of the acute phase of experimental allergic encephalomyelitis. Levels of macrophage chemotactic factor-1 message were also elevated immediately before the onset of clinical signs, peaked with the height of clinical disease, and declined with resolution of the disease. Unlike CSF-1 or c-fms, no endogenous macrophage chemotactic factor-1 message was detected in control animals. Macrophage chemotactic factor-1 protein was demonstrated by Western blot in the central nervous system at the height of clinical disease. The results support the conclusion that expression of factors that specifically target cells of the monocyte/ macrophage series are an important component of the disease process in experimental allergic encephalomyelitis.",
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