Expression of colony-stimulating factor-1 in the human uterus and placenta

Eric Daiter, Serge Pampfer, Y. G. Yeung, David Barad, E. Richard Stanley, Jeffrey W. Pollard

Research output: Contribution to journalArticle

150 Citations (Scopus)

Abstract

Previous studies in the mouse have strongly implicated colony-stimulating factor-1 (CSF-1) in the regulation of placental development. In this study of human pregnancy, we detected CSF-1 in serum, endometrium, placenta, chorion, amnion, and amniotic fluid, with significant increases in serum and endometrial samples from the first trimester compared to levels in nonpregnant controls. CSF-1 mRNA was demonstrated in all of these tissues, except amnion, with a significant increase within the first trimester endometrial samples over nonpregnant control values. In addition to the major 4.0-kilobase mRNA, other species of CSF-1 mRNA were detected, which were shown to be due to alternative splicing within exon 6 and the alternative use of exon 9 or 10. In the endometrium, CSF-1 was localized to glandular epithelial and endothelial cells. In first trimester placenta, CSF-1 was in the cytotrophoblasts lining the villous core and in the cytotrophoblastic shell. During the second trimester, CSF-1 was localized to villous mesenchymal cells. By the third trimester, CSF-1 was only detected in cells lining the villous vessels. The detection of CSF-1 during gestation strongly supports a role for CSF-1 in the regulation of placental function in humans by autocrine and/or paracrine mechanisms.

Original languageEnglish (US)
Pages (from-to)850-858
Number of pages9
JournalJournal of Clinical Endocrinology and Metabolism
Volume74
Issue number4
StatePublished - Apr 1992

Fingerprint

Macrophage Colony-Stimulating Factor
Placenta
Uterus
First Pregnancy Trimester
Amnion
Endometrium
Linings
Messenger RNA
Exons
Chorion
Placentation
Pregnancy
Endothelial cells
Alternative Splicing
Trophoblasts
Third Pregnancy Trimester
Second Pregnancy Trimester
Amniotic Fluid
Serum
Endothelial Cells

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology, Diabetes and Metabolism

Cite this

Daiter, E., Pampfer, S., Yeung, Y. G., Barad, D., Stanley, E. R., & Pollard, J. W. (1992). Expression of colony-stimulating factor-1 in the human uterus and placenta. Journal of Clinical Endocrinology and Metabolism, 74(4), 850-858.

Expression of colony-stimulating factor-1 in the human uterus and placenta. / Daiter, Eric; Pampfer, Serge; Yeung, Y. G.; Barad, David; Stanley, E. Richard; Pollard, Jeffrey W.

In: Journal of Clinical Endocrinology and Metabolism, Vol. 74, No. 4, 04.1992, p. 850-858.

Research output: Contribution to journalArticle

Daiter, E, Pampfer, S, Yeung, YG, Barad, D, Stanley, ER & Pollard, JW 1992, 'Expression of colony-stimulating factor-1 in the human uterus and placenta', Journal of Clinical Endocrinology and Metabolism, vol. 74, no. 4, pp. 850-858.
Daiter E, Pampfer S, Yeung YG, Barad D, Stanley ER, Pollard JW. Expression of colony-stimulating factor-1 in the human uterus and placenta. Journal of Clinical Endocrinology and Metabolism. 1992 Apr;74(4):850-858.
Daiter, Eric ; Pampfer, Serge ; Yeung, Y. G. ; Barad, David ; Stanley, E. Richard ; Pollard, Jeffrey W. / Expression of colony-stimulating factor-1 in the human uterus and placenta. In: Journal of Clinical Endocrinology and Metabolism. 1992 ; Vol. 74, No. 4. pp. 850-858.
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