Expression of carbonic anhydrase IV in carbonic anhydrase II-deficient mice

Luc P. Brion, Wendy Cammer, Lisa M. Satlin, Carlos Suarez, Beth J. Zavilowitz, Victor L. Schuster

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Chronic metabolic acidosis (CMA) in the rabbit upregulates carbonic anhydrase (CA) IV in the proximal convoluted tubule (PCT). This study was designed to assess CA IV expression in a model of CMA in the mouse, i.e., congenital deficiency in CA II [CA(II)D]. In female CA(II)D mice, CA IV specific activity but not CA IV immunoreactivity was upregulated in the renal cortex, specifically in microdissected PCTs. Western blot analysis showed higher expression of CA IV immunoreactive protein in renal membranes from males than in those from females.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Renal Physiology
Volume273
Issue number2 42-2
StatePublished - Aug 1997

Fingerprint

Carbonic Anhydrase IV
Carbonic Anhydrase II
Acidosis
Kidney
Up-Regulation
Western Blotting
Rabbits
Membranes
Proteins

Keywords

  • Affinity chromatography
  • Carbonic anhydrase II deficiency
  • Carbonic anhydrase isozymes I, II, III, and IV
  • Cortex
  • Hydratase activity
  • Liver
  • Lung
  • Outer medulla
  • Regulation
  • Renal tubular acidosis

ASJC Scopus subject areas

  • Physiology

Cite this

Brion, L. P., Cammer, W., Satlin, L. M., Suarez, C., Zavilowitz, B. J., & Schuster, V. L. (1997). Expression of carbonic anhydrase IV in carbonic anhydrase II-deficient mice. American Journal of Physiology - Renal Physiology, 273(2 42-2).

Expression of carbonic anhydrase IV in carbonic anhydrase II-deficient mice. / Brion, Luc P.; Cammer, Wendy; Satlin, Lisa M.; Suarez, Carlos; Zavilowitz, Beth J.; Schuster, Victor L.

In: American Journal of Physiology - Renal Physiology, Vol. 273, No. 2 42-2, 08.1997.

Research output: Contribution to journalArticle

Brion, LP, Cammer, W, Satlin, LM, Suarez, C, Zavilowitz, BJ & Schuster, VL 1997, 'Expression of carbonic anhydrase IV in carbonic anhydrase II-deficient mice', American Journal of Physiology - Renal Physiology, vol. 273, no. 2 42-2.
Brion, Luc P. ; Cammer, Wendy ; Satlin, Lisa M. ; Suarez, Carlos ; Zavilowitz, Beth J. ; Schuster, Victor L. / Expression of carbonic anhydrase IV in carbonic anhydrase II-deficient mice. In: American Journal of Physiology - Renal Physiology. 1997 ; Vol. 273, No. 2 42-2.
@article{4f2614e134f5471ca00e0a71100d253c,
title = "Expression of carbonic anhydrase IV in carbonic anhydrase II-deficient mice",
abstract = "Chronic metabolic acidosis (CMA) in the rabbit upregulates carbonic anhydrase (CA) IV in the proximal convoluted tubule (PCT). This study was designed to assess CA IV expression in a model of CMA in the mouse, i.e., congenital deficiency in CA II [CA(II)D]. In female CA(II)D mice, CA IV specific activity but not CA IV immunoreactivity was upregulated in the renal cortex, specifically in microdissected PCTs. Western blot analysis showed higher expression of CA IV immunoreactive protein in renal membranes from males than in those from females.",
keywords = "Affinity chromatography, Carbonic anhydrase II deficiency, Carbonic anhydrase isozymes I, II, III, and IV, Cortex, Hydratase activity, Liver, Lung, Outer medulla, Regulation, Renal tubular acidosis",
author = "Brion, {Luc P.} and Wendy Cammer and Satlin, {Lisa M.} and Carlos Suarez and Zavilowitz, {Beth J.} and Schuster, {Victor L.}",
year = "1997",
month = "8",
language = "English (US)",
volume = "273",
journal = "American Journal of Physiology - Renal Fluid and Electrolyte Physiology",
issn = "1931-857X",
publisher = "American Physiological Society",
number = "2 42-2",

}

TY - JOUR

T1 - Expression of carbonic anhydrase IV in carbonic anhydrase II-deficient mice

AU - Brion, Luc P.

AU - Cammer, Wendy

AU - Satlin, Lisa M.

AU - Suarez, Carlos

AU - Zavilowitz, Beth J.

AU - Schuster, Victor L.

PY - 1997/8

Y1 - 1997/8

N2 - Chronic metabolic acidosis (CMA) in the rabbit upregulates carbonic anhydrase (CA) IV in the proximal convoluted tubule (PCT). This study was designed to assess CA IV expression in a model of CMA in the mouse, i.e., congenital deficiency in CA II [CA(II)D]. In female CA(II)D mice, CA IV specific activity but not CA IV immunoreactivity was upregulated in the renal cortex, specifically in microdissected PCTs. Western blot analysis showed higher expression of CA IV immunoreactive protein in renal membranes from males than in those from females.

AB - Chronic metabolic acidosis (CMA) in the rabbit upregulates carbonic anhydrase (CA) IV in the proximal convoluted tubule (PCT). This study was designed to assess CA IV expression in a model of CMA in the mouse, i.e., congenital deficiency in CA II [CA(II)D]. In female CA(II)D mice, CA IV specific activity but not CA IV immunoreactivity was upregulated in the renal cortex, specifically in microdissected PCTs. Western blot analysis showed higher expression of CA IV immunoreactive protein in renal membranes from males than in those from females.

KW - Affinity chromatography

KW - Carbonic anhydrase II deficiency

KW - Carbonic anhydrase isozymes I, II, III, and IV

KW - Cortex

KW - Hydratase activity

KW - Liver

KW - Lung

KW - Outer medulla

KW - Regulation

KW - Renal tubular acidosis

UR - http://www.scopus.com/inward/record.url?scp=0030846260&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030846260&partnerID=8YFLogxK

M3 - Article

VL - 273

JO - American Journal of Physiology - Renal Fluid and Electrolyte Physiology

JF - American Journal of Physiology - Renal Fluid and Electrolyte Physiology

SN - 1931-857X

IS - 2 42-2

ER -