Abstract
Crystallins are very abundant structural proteins of the lens and are also expressed in other tissues. We have previously reported a spontaneous mutation in the rat βA3/A1-crystallin gene, termed Nuc1, which has a novel, complex, ocular phenotype. The current study was undertaken to compare the expression pattern of this gene during eye development in wild type and Nuc1 rats by in situ hybridization (ISH) and immunohistochemistry (IHC). βA3/A1-crystallin expression was first detected in the eyes of both wild type and Nuc1 rats at embryonic (E) day 12.5 in the posterior portion of the lens vesicle, and remained limited to the lens fibers throughout fetal life. After birth, βA3/A1-crystallin expression was also detected in the neural retina (specifically in the astrocytes and ganglion cells) and in the retinal pigmented epithelium (RPE). This suggested that βA3/A1-crystallin is not only a structural protein of the lens, but has cellular function(s) in other ocular tissues. In summary, expression of βA3/A1-crystallin is controlled differentially in various eye tissues with lens being the site of greatest expression. Similar staining patterns, detected by ISH and IHC, in wild type and Nuc1 animals suggest that functional differences in the protein, rather than changes in mRNA/protein level of expression, likely account for developmental abnormalities in Nuc1.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 59-69 |
| Number of pages | 11 |
| Journal | Journal of Molecular Histology |
| Volume | 42 |
| Issue number | 1 |
| DOIs | |
| State | Published - Feb 2011 |
| Externally published | Yes |
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Keywords
- βA3/A1crystallin
- Astrocytes
- Eye development
- Ganglion cells
- In situ hybridization
- Lens
- Retina
ASJC Scopus subject areas
- Cell Biology
- Histology
- Physiology
Cite this
Expression of βa3/A1-crystallin in the developing and adult rat eye. / Parthasarathy, Geetha; Ma, Bo; Zhang, Cheng; Gongora, Celine; Samuel Zigler, J.; Duncan, Melinda K.; Sinha, Debasish.
In: Journal of Molecular Histology, Vol. 42, No. 1, 02.2011, p. 59-69.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Expression of βa3/A1-crystallin in the developing and adult rat eye
AU - Parthasarathy, Geetha
AU - Ma, Bo
AU - Zhang, Cheng
AU - Gongora, Celine
AU - Samuel Zigler, J.
AU - Duncan, Melinda K.
AU - Sinha, Debasish
PY - 2011/2
Y1 - 2011/2
N2 - Crystallins are very abundant structural proteins of the lens and are also expressed in other tissues. We have previously reported a spontaneous mutation in the rat βA3/A1-crystallin gene, termed Nuc1, which has a novel, complex, ocular phenotype. The current study was undertaken to compare the expression pattern of this gene during eye development in wild type and Nuc1 rats by in situ hybridization (ISH) and immunohistochemistry (IHC). βA3/A1-crystallin expression was first detected in the eyes of both wild type and Nuc1 rats at embryonic (E) day 12.5 in the posterior portion of the lens vesicle, and remained limited to the lens fibers throughout fetal life. After birth, βA3/A1-crystallin expression was also detected in the neural retina (specifically in the astrocytes and ganglion cells) and in the retinal pigmented epithelium (RPE). This suggested that βA3/A1-crystallin is not only a structural protein of the lens, but has cellular function(s) in other ocular tissues. In summary, expression of βA3/A1-crystallin is controlled differentially in various eye tissues with lens being the site of greatest expression. Similar staining patterns, detected by ISH and IHC, in wild type and Nuc1 animals suggest that functional differences in the protein, rather than changes in mRNA/protein level of expression, likely account for developmental abnormalities in Nuc1.
AB - Crystallins are very abundant structural proteins of the lens and are also expressed in other tissues. We have previously reported a spontaneous mutation in the rat βA3/A1-crystallin gene, termed Nuc1, which has a novel, complex, ocular phenotype. The current study was undertaken to compare the expression pattern of this gene during eye development in wild type and Nuc1 rats by in situ hybridization (ISH) and immunohistochemistry (IHC). βA3/A1-crystallin expression was first detected in the eyes of both wild type and Nuc1 rats at embryonic (E) day 12.5 in the posterior portion of the lens vesicle, and remained limited to the lens fibers throughout fetal life. After birth, βA3/A1-crystallin expression was also detected in the neural retina (specifically in the astrocytes and ganglion cells) and in the retinal pigmented epithelium (RPE). This suggested that βA3/A1-crystallin is not only a structural protein of the lens, but has cellular function(s) in other ocular tissues. In summary, expression of βA3/A1-crystallin is controlled differentially in various eye tissues with lens being the site of greatest expression. Similar staining patterns, detected by ISH and IHC, in wild type and Nuc1 animals suggest that functional differences in the protein, rather than changes in mRNA/protein level of expression, likely account for developmental abnormalities in Nuc1.
KW - βA3/A1crystallin
KW - Astrocytes
KW - Eye development
KW - Ganglion cells
KW - In situ hybridization
KW - Lens
KW - Retina
UR - http://www.scopus.com/inward/record.url?scp=79951828465&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=79951828465&partnerID=8YFLogxK
U2 - 10.1007/s10735-010-9307-1
DO - 10.1007/s10735-010-9307-1
M3 - Article
C2 - 21203897
AN - SCOPUS:79951828465
VL - 42
SP - 59
EP - 69
JO - Journal of Molecular Histology
JF - Journal of Molecular Histology
SN - 1567-2379
IS - 1
ER -