Expression and function of a putative KATP channel subtype in human corpus cavernosum smooth muscle

N. S. Day, A. Giraldi, W. Zhao, A. Melman, F. Staerman, D. C. Kim, M. Spektor, C. M. Gondrs, F. T. Murray, S. Lee, G. J. Christ

Research output: Contribution to journalArticle

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Abstract

K channels are important modulators of human corporal smooth muscle tone. The goals of this study were 3-fold: 1. to study the expression of the KATP channel in corporal tissue and cultured cells; 2. to determine if K channels are involved in the relaxation responses elicited by putative K channel modulators, & 3. to determine if K channel modulators alter intracellular calcium mobilization. Western blots and immunocytochemistry revealed abundant KATP channel expression in both frozen tissues and cultured cells. Cumulative concentration response curves (CRCs) were constructed for relaxation of PE-precontracted corporal tissue strips by Pinacidil (PIN), P1075, and U99751; which elicited relaxation responses with ≈EC50 values of of 3 nM, 60 nM, and 30 nM, respectively. Preincubation with glibenclamide (GLIB, 1 μM: 30 mins) partially inhibited the relaxation responses elicited by all of these putative K channel openers. Relaxation responses to P1075 were completely and immediately reversed by addition of KCl (60 mM), and more slowly, but completely by TEA (100 mM) or GLIB (1 μM). Calcium imaging experiments on fura-2 loaded cultured smooth muscle cells revealed that preincubation with (PIN) (1 μM) elicited a significant and completely GLIB-sensitive (10 μM) diminution in the peak amplitude of the ET-1 (endothelin-1)-induced intracellular calcium transient; which was equivalent to that observed in the presence of verapamil (1 μM), a L-type calcium channel blocker, or in the absence of extracellular calcium. These data strongly suggest the involvement of K channels (KATP) in this relaxation.

Original languageEnglish (US)
JournalFASEB Journal
Volume11
Issue number3
StatePublished - 1997

Fingerprint

KATP Channels
smooth muscle
Smooth Muscle
Muscle
Modulators
Calcium
calcium
Cells
Tissue
cultured cells
Cultured Cells
Pinacidil
glibenclamide
calcium channel blockers
L-Type Calcium Channels
endothelins
verapamil
Glyburide
Fura-2
Calcium Channel Blockers

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Day, N. S., Giraldi, A., Zhao, W., Melman, A., Staerman, F., Kim, D. C., ... Christ, G. J. (1997). Expression and function of a putative KATP channel subtype in human corpus cavernosum smooth muscle. FASEB Journal, 11(3).

Expression and function of a putative KATP channel subtype in human corpus cavernosum smooth muscle. / Day, N. S.; Giraldi, A.; Zhao, W.; Melman, A.; Staerman, F.; Kim, D. C.; Spektor, M.; Gondrs, C. M.; Murray, F. T.; Lee, S.; Christ, G. J.

In: FASEB Journal, Vol. 11, No. 3, 1997.

Research output: Contribution to journalArticle

Day, NS, Giraldi, A, Zhao, W, Melman, A, Staerman, F, Kim, DC, Spektor, M, Gondrs, CM, Murray, FT, Lee, S & Christ, GJ 1997, 'Expression and function of a putative KATP channel subtype in human corpus cavernosum smooth muscle', FASEB Journal, vol. 11, no. 3.
Day NS, Giraldi A, Zhao W, Melman A, Staerman F, Kim DC et al. Expression and function of a putative KATP channel subtype in human corpus cavernosum smooth muscle. FASEB Journal. 1997;11(3).
Day, N. S. ; Giraldi, A. ; Zhao, W. ; Melman, A. ; Staerman, F. ; Kim, D. C. ; Spektor, M. ; Gondrs, C. M. ; Murray, F. T. ; Lee, S. ; Christ, G. J. / Expression and function of a putative KATP channel subtype in human corpus cavernosum smooth muscle. In: FASEB Journal. 1997 ; Vol. 11, No. 3.
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AU - Kim, D. C.

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AU - Lee, S.

AU - Christ, G. J.

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