TY - JOUR
T1 - Exploring the mycobacteriophage metaproteome
T2 - Phage genomics as an educational platform
AU - Hatfull, Graham F.
AU - Pedulla, Marisa L.
AU - Jacobs-Sera, Deborah
AU - Cichon, Pauline M.
AU - Foley, Amy
AU - Ford, Michael E.
AU - Gonda, Rebecca M.
AU - Houtz, Jennifer M.
AU - Hryckowian, Andrew J.
AU - Kelchner, Vanessa A.
AU - Namburi, Swathi
AU - Pajcini, Kostandin V.
AU - Popovich, Mark G.
AU - Schleicher, Donald T.
AU - Simanek, Brian Z.
AU - Smith, Alexis L.
AU - Zdanowicz, Gina M.
AU - Kumar, Vanaja
AU - Peebles, Craig L.
AU - Jacobs, William R.
AU - Lawrence, Jeffrey G.
AU - Hendrix, Roger W.
PY - 2006
Y1 - 2006
N2 - Bacteriophages are the most abundant forms of life in the biosphere and carry genomes characterized by high genetic diversity and mosaic architectures. The complete sequences of 30 mycobacteriophage genomes show them collectively to encode 101 tRNAs, three tmRNAs, and 3,357 proteins belonging to 1,536 "phamilies" of related sequences, and a statistical analysis predicts that these represent approximately 50% of the total number of phamilies in the mycobacteriophage population. These phamilies contain 2.19 proteins on average; more than half (774) of them contain just a single protein sequence. Only six phamilies have representatives in more than half of the 30 genomes, and only three - encoding tape-measure proteins, lysins, and minor tail proteins - are present in all 30 phages, although these phamilies are themselves highly modular, such that no single amino acid sequence element is present in all 30 mycobacteriophage genomes. Of the 1,536 phamilies, only 230 (15%) have amino acid sequence similarity to previously reported proteins, reflecting the enormous genetic diversity of the entire phage population. The abundance and diversity of phages, the simplicity of phage isolation, and the relatively small size of phage genomes support bacteriophage isolation and comparative genomic analysis as a highly suitable platform for discovery-based education.
AB - Bacteriophages are the most abundant forms of life in the biosphere and carry genomes characterized by high genetic diversity and mosaic architectures. The complete sequences of 30 mycobacteriophage genomes show them collectively to encode 101 tRNAs, three tmRNAs, and 3,357 proteins belonging to 1,536 "phamilies" of related sequences, and a statistical analysis predicts that these represent approximately 50% of the total number of phamilies in the mycobacteriophage population. These phamilies contain 2.19 proteins on average; more than half (774) of them contain just a single protein sequence. Only six phamilies have representatives in more than half of the 30 genomes, and only three - encoding tape-measure proteins, lysins, and minor tail proteins - are present in all 30 phages, although these phamilies are themselves highly modular, such that no single amino acid sequence element is present in all 30 mycobacteriophage genomes. Of the 1,536 phamilies, only 230 (15%) have amino acid sequence similarity to previously reported proteins, reflecting the enormous genetic diversity of the entire phage population. The abundance and diversity of phages, the simplicity of phage isolation, and the relatively small size of phage genomes support bacteriophage isolation and comparative genomic analysis as a highly suitable platform for discovery-based education.
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U2 - 10.1371/journal.pgen.0020092
DO - 10.1371/journal.pgen.0020092
M3 - Article
C2 - 16789831
AN - SCOPUS:33745698561
SN - 1553-7390
VL - 2
SP - 835
EP - 847
JO - PLoS genetics
JF - PLoS genetics
IS - 6
ER -
