Exploring the mycobacteriophage metaproteome: Phage genomics as an educational platform

Graham F. Hatfull, Marisa L. Pedulla, Deborah Jacobs-Sera, Pauline M. Cichon, Amy Foley, Michael E. Ford, Rebecca M. Gonda, Jennifer M. Houtz, Andrew J. Hryckowian, Vanessa A. Kelchner, Swathi Namburi, Kostandin V. Pajcini, Mark G. Popovich, Donald T. Schleicher, Brian Z. Simanek, Alexis L. Smith, Gina M. Zdanowicz, Vanaja Kumar, Craig L. Peebles, William R. JacobsJeffrey G. Lawrence, Roger W. Hendrix

Research output: Contribution to journalArticle

Abstract

Bacteriophages are the most abundant forms of life in the biosphere and carry genomes characterized by high genetic diversity and mosaic architectures. The complete sequences of 30 mycobacteriophage genomes show them collectively to encode 101 tRNAs, three tmRNAs, and 3,357 proteins belonging to 1,536 "phamilies" of related sequences, and a statistical analysis predicts that these represent approximately 50% of the total number of phamilies in the mycobacteriophage population. These phamilies contain 2.19 proteins on average; more than half (774) of them contain just a single protein sequence. Only six phamilies have representatives in more than half of the 30 genomes, and only three - encoding tape-measure proteins, lysins, and minor tail proteins - are present in all 30 phages, although these phamilies are themselves highly modular, such that no single amino acid sequence element is present in all 30 mycobacteriophage genomes. Of the 1,536 phamilies, only 230 (15%) have amino acid sequence similarity to previously reported proteins, reflecting the enormous genetic diversity of the entire phage population. The abundance and diversity of phages, the simplicity of phage isolation, and the relatively small size of phage genomes support bacteriophage isolation and comparative genomic analysis as a highly suitable platform for discovery-based education.

Original languageEnglish (US)
Pages (from-to)835-847
Number of pages13
JournalPLoS genetics
Volume2
Issue number6
DOIs
StatePublished - Jul 11 2006

Fingerprint

Mycobacteriophages
Genomics
bacteriophages
Bacteriophages
genomics
genome
protein
Genome
bacteriophage
Proteins
amino acid sequences
proteins
amino acid
Amino Acid Sequence
Genome Size
genetic variation
biosphere
Transfer RNA
mycobacteriophage
statistical analysis

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Molecular Biology
  • Genetics
  • Genetics(clinical)
  • Cancer Research

Cite this

Hatfull, G. F., Pedulla, M. L., Jacobs-Sera, D., Cichon, P. M., Foley, A., Ford, M. E., ... Hendrix, R. W. (2006). Exploring the mycobacteriophage metaproteome: Phage genomics as an educational platform. PLoS genetics, 2(6), 835-847. https://doi.org/10.1371/journal.pgen.0020092

Exploring the mycobacteriophage metaproteome : Phage genomics as an educational platform. / Hatfull, Graham F.; Pedulla, Marisa L.; Jacobs-Sera, Deborah; Cichon, Pauline M.; Foley, Amy; Ford, Michael E.; Gonda, Rebecca M.; Houtz, Jennifer M.; Hryckowian, Andrew J.; Kelchner, Vanessa A.; Namburi, Swathi; Pajcini, Kostandin V.; Popovich, Mark G.; Schleicher, Donald T.; Simanek, Brian Z.; Smith, Alexis L.; Zdanowicz, Gina M.; Kumar, Vanaja; Peebles, Craig L.; Jacobs, William R.; Lawrence, Jeffrey G.; Hendrix, Roger W.

In: PLoS genetics, Vol. 2, No. 6, 11.07.2006, p. 835-847.

Research output: Contribution to journalArticle

Hatfull, GF, Pedulla, ML, Jacobs-Sera, D, Cichon, PM, Foley, A, Ford, ME, Gonda, RM, Houtz, JM, Hryckowian, AJ, Kelchner, VA, Namburi, S, Pajcini, KV, Popovich, MG, Schleicher, DT, Simanek, BZ, Smith, AL, Zdanowicz, GM, Kumar, V, Peebles, CL, Jacobs, WR, Lawrence, JG & Hendrix, RW 2006, 'Exploring the mycobacteriophage metaproteome: Phage genomics as an educational platform', PLoS genetics, vol. 2, no. 6, pp. 835-847. https://doi.org/10.1371/journal.pgen.0020092
Hatfull GF, Pedulla ML, Jacobs-Sera D, Cichon PM, Foley A, Ford ME et al. Exploring the mycobacteriophage metaproteome: Phage genomics as an educational platform. PLoS genetics. 2006 Jul 11;2(6):835-847. https://doi.org/10.1371/journal.pgen.0020092
Hatfull, Graham F. ; Pedulla, Marisa L. ; Jacobs-Sera, Deborah ; Cichon, Pauline M. ; Foley, Amy ; Ford, Michael E. ; Gonda, Rebecca M. ; Houtz, Jennifer M. ; Hryckowian, Andrew J. ; Kelchner, Vanessa A. ; Namburi, Swathi ; Pajcini, Kostandin V. ; Popovich, Mark G. ; Schleicher, Donald T. ; Simanek, Brian Z. ; Smith, Alexis L. ; Zdanowicz, Gina M. ; Kumar, Vanaja ; Peebles, Craig L. ; Jacobs, William R. ; Lawrence, Jeffrey G. ; Hendrix, Roger W. / Exploring the mycobacteriophage metaproteome : Phage genomics as an educational platform. In: PLoS genetics. 2006 ; Vol. 2, No. 6. pp. 835-847.
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