Exploring human antimicrobial antibody responses on a single B cell level

Daniel Hofmann, Jonathan R. Lai

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Analysis of monoclonal antibodies (MAbs) derived from single B cell cloning has been highly beneficial for antimicrobial immunotherapy, vaccine design, and advancing our understanding of pathogen-triggered effects on the human immunoglobulin repertoire. Sequencing of variable domains of single B cells, and characterization of binding and functional activities of MAbs derived from those sequences, provides in-depth insight not only into sites of susceptibility for antibodymediated neutralization or opsonization of the pathogen but also into the dynamics of protective antibody evolution during infection. This information can be utilized to rapidly develop novel immunotherapies of completely human origin and provides a roadmap for structure-based vaccine design that aims to elicit similar protective antibody responses. Here, we summarize recent aspects of the single B cell cloning approach.

Original languageEnglish (US)
Article numbere00544
JournalClinical and Vaccine Immunology
Volume24
Issue number5
DOIs
StatePublished - May 1 2017

Fingerprint

Antibody Formation
B-Lymphocytes
Cloning
Cells
Pathogens
Immunotherapy
Antibodies
Organism Cloning
Vaccines
Monoclonal Antibodies
Immunoglobulins
Infection

Keywords

  • Antibody discovery
  • Immune responses
  • Immunotherapy
  • Single B cell sorting

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Clinical Biochemistry
  • Microbiology (medical)

Cite this

Exploring human antimicrobial antibody responses on a single B cell level. / Hofmann, Daniel; Lai, Jonathan R.

In: Clinical and Vaccine Immunology, Vol. 24, No. 5, e00544, 01.05.2017.

Research output: Contribution to journalArticle

@article{3fdcb95444c64b66b652678267a716ff,
title = "Exploring human antimicrobial antibody responses on a single B cell level",
abstract = "Analysis of monoclonal antibodies (MAbs) derived from single B cell cloning has been highly beneficial for antimicrobial immunotherapy, vaccine design, and advancing our understanding of pathogen-triggered effects on the human immunoglobulin repertoire. Sequencing of variable domains of single B cells, and characterization of binding and functional activities of MAbs derived from those sequences, provides in-depth insight not only into sites of susceptibility for antibodymediated neutralization or opsonization of the pathogen but also into the dynamics of protective antibody evolution during infection. This information can be utilized to rapidly develop novel immunotherapies of completely human origin and provides a roadmap for structure-based vaccine design that aims to elicit similar protective antibody responses. Here, we summarize recent aspects of the single B cell cloning approach.",
keywords = "Antibody discovery, Immune responses, Immunotherapy, Single B cell sorting",
author = "Daniel Hofmann and Lai, {Jonathan R.}",
year = "2017",
month = "5",
day = "1",
doi = "10.1128/CVI.00544-16",
language = "English (US)",
volume = "24",
journal = "Clinical and Vaccine Immunology",
issn = "1556-6811",
publisher = "American Society for Microbiology",
number = "5",

}

TY - JOUR

T1 - Exploring human antimicrobial antibody responses on a single B cell level

AU - Hofmann, Daniel

AU - Lai, Jonathan R.

PY - 2017/5/1

Y1 - 2017/5/1

N2 - Analysis of monoclonal antibodies (MAbs) derived from single B cell cloning has been highly beneficial for antimicrobial immunotherapy, vaccine design, and advancing our understanding of pathogen-triggered effects on the human immunoglobulin repertoire. Sequencing of variable domains of single B cells, and characterization of binding and functional activities of MAbs derived from those sequences, provides in-depth insight not only into sites of susceptibility for antibodymediated neutralization or opsonization of the pathogen but also into the dynamics of protective antibody evolution during infection. This information can be utilized to rapidly develop novel immunotherapies of completely human origin and provides a roadmap for structure-based vaccine design that aims to elicit similar protective antibody responses. Here, we summarize recent aspects of the single B cell cloning approach.

AB - Analysis of monoclonal antibodies (MAbs) derived from single B cell cloning has been highly beneficial for antimicrobial immunotherapy, vaccine design, and advancing our understanding of pathogen-triggered effects on the human immunoglobulin repertoire. Sequencing of variable domains of single B cells, and characterization of binding and functional activities of MAbs derived from those sequences, provides in-depth insight not only into sites of susceptibility for antibodymediated neutralization or opsonization of the pathogen but also into the dynamics of protective antibody evolution during infection. This information can be utilized to rapidly develop novel immunotherapies of completely human origin and provides a roadmap for structure-based vaccine design that aims to elicit similar protective antibody responses. Here, we summarize recent aspects of the single B cell cloning approach.

KW - Antibody discovery

KW - Immune responses

KW - Immunotherapy

KW - Single B cell sorting

UR - http://www.scopus.com/inward/record.url?scp=85019155261&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85019155261&partnerID=8YFLogxK

U2 - 10.1128/CVI.00544-16

DO - 10.1128/CVI.00544-16

M3 - Article

C2 - 28356257

AN - SCOPUS:85019155261

VL - 24

JO - Clinical and Vaccine Immunology

JF - Clinical and Vaccine Immunology

SN - 1556-6811

IS - 5

M1 - e00544

ER -