Exploring human antimicrobial antibody responses on a single B cell level

Daniel Hofmann, Jonathan R. Lai

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Analysis of monoclonal antibodies (MAbs) derived from single B cell cloning has been highly beneficial for antimicrobial immunotherapy, vaccine design, and advancing our understanding of pathogen-triggered effects on the human immunoglobulin repertoire. Sequencing of variable domains of single B cells, and characterization of binding and functional activities of MAbs derived from those sequences, provides in-depth insight not only into sites of susceptibility for antibodymediated neutralization or opsonization of the pathogen but also into the dynamics of protective antibody evolution during infection. This information can be utilized to rapidly develop novel immunotherapies of completely human origin and provides a roadmap for structure-based vaccine design that aims to elicit similar protective antibody responses. Here, we summarize recent aspects of the single B cell cloning approach.

Original languageEnglish (US)
Article numbere00544
JournalClinical and Vaccine Immunology
Volume24
Issue number5
DOIs
StatePublished - May 2017

Keywords

  • Antibody discovery
  • Immune responses
  • Immunotherapy
  • Single B cell sorting

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Clinical Biochemistry
  • Microbiology (medical)

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