Experimental murine acid aspiration injury is mediated by neutrophils and the alternative complement pathway

Martin R. Weiser, Taine T V Pechet, Julian P. Williams, Minghe Ma, Paul S. Frenette, Francis D. Moore, Lester Kobzik, Richard O. Hines, Denisa D. Wagner, Michael C. Carroll, Herbert B. Hechtman

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Acid aspiration may result in the development of the acute respiratory distress syndrome, an event associated with significant morbidity and mortality. Although once attributed to direct distal airway injury, the pulmonary failure after acid aspiration is more complex and involves an inflammatory injury mediated by complement (C) and polymorphonuclear leukocytes. This study examines the injurious inflammatory cascades that are activated after acid aspiration. The role of neutrophils was defined by immunodepletion before aspiration, which reduced injury by 59%. The injury was not modified in either P- or E-selectin-knockout mice, indicating that these adhesion molecules were not operative. C activation after aspiration was documented with immunochemistry by C3 deposition on injured alveolar pneumocytes. Animals in which C activation was inhibited with soluble C receptor type 1 (sCR1) had a 54% reduction in injury, similar to the level of protection seen in C3-knockout mice (58%). However C4-knockout mice were not protected from injury, indicating that C activation is mediated by the alternative pathway. Finally, an additive effect of neutrophils and C was demonstrated whereby neutropenic animals that were treated with sCR1 showed an 85% reduction in injury. Thus acid aspiration injury is mediated by neutrophils and the alternative C pathway.

Original languageEnglish (US)
Pages (from-to)1090-1095
Number of pages6
JournalJournal of Applied Physiology
Volume83
Issue number4
StatePublished - Oct 1997
Externally publishedYes

Fingerprint

Alternative Complement Pathway
Neutrophils
Acids
Wounds and Injuries
Knockout Mice
Alveolar Epithelial Cells
Immunochemistry
E-Selectin
Adult Respiratory Distress Syndrome
Lung Injury
Morbidity
Mortality

Keywords

  • Complement activation
  • Inflammation
  • Pneumonia
  • Selectins
  • Transgenic mice

ASJC Scopus subject areas

  • Physiology
  • Endocrinology
  • Orthopedics and Sports Medicine
  • Physical Therapy, Sports Therapy and Rehabilitation

Cite this

Weiser, M. R., Pechet, T. T. V., Williams, J. P., Ma, M., Frenette, P. S., Moore, F. D., ... Hechtman, H. B. (1997). Experimental murine acid aspiration injury is mediated by neutrophils and the alternative complement pathway. Journal of Applied Physiology, 83(4), 1090-1095.

Experimental murine acid aspiration injury is mediated by neutrophils and the alternative complement pathway. / Weiser, Martin R.; Pechet, Taine T V; Williams, Julian P.; Ma, Minghe; Frenette, Paul S.; Moore, Francis D.; Kobzik, Lester; Hines, Richard O.; Wagner, Denisa D.; Carroll, Michael C.; Hechtman, Herbert B.

In: Journal of Applied Physiology, Vol. 83, No. 4, 10.1997, p. 1090-1095.

Research output: Contribution to journalArticle

Weiser, MR, Pechet, TTV, Williams, JP, Ma, M, Frenette, PS, Moore, FD, Kobzik, L, Hines, RO, Wagner, DD, Carroll, MC & Hechtman, HB 1997, 'Experimental murine acid aspiration injury is mediated by neutrophils and the alternative complement pathway', Journal of Applied Physiology, vol. 83, no. 4, pp. 1090-1095.
Weiser, Martin R. ; Pechet, Taine T V ; Williams, Julian P. ; Ma, Minghe ; Frenette, Paul S. ; Moore, Francis D. ; Kobzik, Lester ; Hines, Richard O. ; Wagner, Denisa D. ; Carroll, Michael C. ; Hechtman, Herbert B. / Experimental murine acid aspiration injury is mediated by neutrophils and the alternative complement pathway. In: Journal of Applied Physiology. 1997 ; Vol. 83, No. 4. pp. 1090-1095.
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