Experimental methods for functional studies of microRNAs in animal models of psychiatric disorders

Vladimir Jovasevic, Jelena Radulovic

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Pharmacological treatments for psychiatric illnesses are often unsuccessful. This is largely due to the poor understanding of the molecular mechanisms underlying these disorders. We are particularly interested in elucidating the mechanism of affective disorders rooted in traumatic experiences. To date, the research of mental disorders in general has focused on the causal role of individual genes and proteins, an approach that is inconsistent with the proposed polygenetic nature of these disorders. We recently took an alternative direction, by establishing the role of miRNAs in the coding of stress-related, fear-provoking memories. Here we describe in detail our work on the role of miR-33 in state-dependent learning, a process implicated in dissociative amnesia, wherein memories formed in a certain brain state can best be retrieved if the brain is in the same state. We present the specific experimental approaches we apply to study the role of miRNAs in this model and demonstrate that miR-33 regulates the susceptibility to state-dependent learning induced by inhibitory neurotransmission.

Original languageEnglish (US)
Title of host publicationNeuromethods
PublisherHumana Press Inc.
Pages129-146
Number of pages18
DOIs
StatePublished - 2017
Externally publishedYes

Publication series

NameNeuromethods
Volume128
ISSN (Print)0893-2336
ISSN (Electronic)1940-6045

Keywords

  • Behavior
  • Hippocampus
  • Learning
  • Lentiviral vectors
  • LNA inhibitors
  • microRNAs
  • miR-33

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)
  • Psychiatry and Mental health

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