Exosomal markers (CD63 and CD9) expression pattern using immunohistochemistry in resected malignant and nonmalignant pancreatic specimens

Moh'd Khushman; Arun Bhardwaj; Girijesh Kumar Patel; Javier Ariel Laurini; Kelly Roveda; Marcus C. Tan; Mary C. Patton; Seema Singh; William Taylor; Ajay P. Singh

doi:10.1097/MPA.0000000000000847

Exosomal markers (CD63 and CD9) expression pattern using immunohistochemistry in resected malignant and nonmalignant pancreatic specimens

Moh'd Khushman, Arun Bhardwaj, Girijesh Kumar Patel, Javier Ariel Laurini, Kelly Roveda, Marcus C. Tan, Mary C. Patton, Seema Singh, William Taylor, Ajay P. Singh

Research output: Contribution to journal › Article › peer-review

66 Scopus citations

Abstract

Objectives: Exosomes are importantmediators in intercellular communications and play a role in cancer progression and metastasis. Exosomal membranes are enriched in endosome-specific tetraspanins (CD9 and CD63). Here, we explored the expression of CD63 and CD9 utilizing immunohistochemistry in malignant and nonmalignant cells in 29 resected pancreatic specimens (RPSs) of mixed racial background. Methods: The pathologic tissues (PTs) and adjacent normal tissues (ANTs) in each RPS were stained for CD63 and CD9. Two pathologists independently scored the expression of CD63 and CD9. Staining intensity was graded from 1 to 3. Staining percentage was estimated in 10% increments. An average Quick score (Q score) (intensity - percentage of staining) was calculated. Unpaired t test was used for statistical analysis. Results: The mean multiplicative Q score for CD63 and CD9 expression is higher in PTs (209 and 72) compared with ANTs (154 and 24) (P = 0.0041, P = 0.0018), respectively. The Mean Q score for CD63 and CD9 expression is higher in themalignant PTs (231 and 85) comparedwith ANTs (129 and 25) (P > 0.0001 and P > 0.0124). Conclusions: Exosomal markers (CD63 and CD9) expression assessment using immunohistochemistry is feasible in RPS. The expression of CD63 and CD9 is higher in PTs and malignant PTs compared with their ANTs.

Original language	English (US)
Pages (from-to)	782-788
Number of pages	7
Journal	Pancreas
Volume	46
Issue number	6
DOIs	https://doi.org/10.1097/MPA.0000000000000847
State	Published - 2017
Externally published	Yes

Keywords

CD63
CD9
Exosomalmarkers
Exosomes
Pancreatic tissues

ASJC Scopus subject areas

Internal Medicine
Endocrinology, Diabetes and Metabolism
Hepatology
Endocrinology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1097/MPA.0000000000000847

Cite this

@article{d26ad94a3f7342d592aefba4007808af,

title = "Exosomal markers (CD63 and CD9) expression pattern using immunohistochemistry in resected malignant and nonmalignant pancreatic specimens",

abstract = "Objectives: Exosomes are importantmediators in intercellular communications and play a role in cancer progression and metastasis. Exosomal membranes are enriched in endosome-specific tetraspanins (CD9 and CD63). Here, we explored the expression of CD63 and CD9 utilizing immunohistochemistry in malignant and nonmalignant cells in 29 resected pancreatic specimens (RPSs) of mixed racial background. Methods: The pathologic tissues (PTs) and adjacent normal tissues (ANTs) in each RPS were stained for CD63 and CD9. Two pathologists independently scored the expression of CD63 and CD9. Staining intensity was graded from 1 to 3. Staining percentage was estimated in 10% increments. An average Quick score (Q score) (intensity - percentage of staining) was calculated. Unpaired t test was used for statistical analysis. Results: The mean multiplicative Q score for CD63 and CD9 expression is higher in PTs (209 and 72) compared with ANTs (154 and 24) (P = 0.0041, P = 0.0018), respectively. The Mean Q score for CD63 and CD9 expression is higher in themalignant PTs (231 and 85) comparedwith ANTs (129 and 25) (P > 0.0001 and P > 0.0124). Conclusions: Exosomal markers (CD63 and CD9) expression assessment using immunohistochemistry is feasible in RPS. The expression of CD63 and CD9 is higher in PTs and malignant PTs compared with their ANTs.",

keywords = "CD63, CD9, Exosomalmarkers, Exosomes, Pancreatic tissues",

author = "Moh'd Khushman and Arun Bhardwaj and Patel, {Girijesh Kumar} and Laurini, {Javier Ariel} and Kelly Roveda and Tan, {Marcus C.} and Patton, {Mary C.} and Seema Singh and William Taylor and Singh, {Ajay P.}",

year = "2017",

doi = "10.1097/MPA.0000000000000847",

language = "English (US)",

volume = "46",

pages = "782--788",

journal = "Pancreas",

issn = "0885-3177",

publisher = "Lippincott Williams and Wilkins",

number = "6",

}

TY - JOUR

T1 - Exosomal markers (CD63 and CD9) expression pattern using immunohistochemistry in resected malignant and nonmalignant pancreatic specimens

AU - Khushman, Moh'd

AU - Bhardwaj, Arun

AU - Patel, Girijesh Kumar

AU - Laurini, Javier Ariel

AU - Roveda, Kelly

AU - Tan, Marcus C.

AU - Patton, Mary C.

AU - Singh, Seema

AU - Taylor, William

AU - Singh, Ajay P.

PY - 2017

Y1 - 2017

N2 - Objectives: Exosomes are importantmediators in intercellular communications and play a role in cancer progression and metastasis. Exosomal membranes are enriched in endosome-specific tetraspanins (CD9 and CD63). Here, we explored the expression of CD63 and CD9 utilizing immunohistochemistry in malignant and nonmalignant cells in 29 resected pancreatic specimens (RPSs) of mixed racial background. Methods: The pathologic tissues (PTs) and adjacent normal tissues (ANTs) in each RPS were stained for CD63 and CD9. Two pathologists independently scored the expression of CD63 and CD9. Staining intensity was graded from 1 to 3. Staining percentage was estimated in 10% increments. An average Quick score (Q score) (intensity - percentage of staining) was calculated. Unpaired t test was used for statistical analysis. Results: The mean multiplicative Q score for CD63 and CD9 expression is higher in PTs (209 and 72) compared with ANTs (154 and 24) (P = 0.0041, P = 0.0018), respectively. The Mean Q score for CD63 and CD9 expression is higher in themalignant PTs (231 and 85) comparedwith ANTs (129 and 25) (P > 0.0001 and P > 0.0124). Conclusions: Exosomal markers (CD63 and CD9) expression assessment using immunohistochemistry is feasible in RPS. The expression of CD63 and CD9 is higher in PTs and malignant PTs compared with their ANTs.

AB - Objectives: Exosomes are importantmediators in intercellular communications and play a role in cancer progression and metastasis. Exosomal membranes are enriched in endosome-specific tetraspanins (CD9 and CD63). Here, we explored the expression of CD63 and CD9 utilizing immunohistochemistry in malignant and nonmalignant cells in 29 resected pancreatic specimens (RPSs) of mixed racial background. Methods: The pathologic tissues (PTs) and adjacent normal tissues (ANTs) in each RPS were stained for CD63 and CD9. Two pathologists independently scored the expression of CD63 and CD9. Staining intensity was graded from 1 to 3. Staining percentage was estimated in 10% increments. An average Quick score (Q score) (intensity - percentage of staining) was calculated. Unpaired t test was used for statistical analysis. Results: The mean multiplicative Q score for CD63 and CD9 expression is higher in PTs (209 and 72) compared with ANTs (154 and 24) (P = 0.0041, P = 0.0018), respectively. The Mean Q score for CD63 and CD9 expression is higher in themalignant PTs (231 and 85) comparedwith ANTs (129 and 25) (P > 0.0001 and P > 0.0124). Conclusions: Exosomal markers (CD63 and CD9) expression assessment using immunohistochemistry is feasible in RPS. The expression of CD63 and CD9 is higher in PTs and malignant PTs compared with their ANTs.

KW - CD63

KW - CD9

KW - Exosomalmarkers

KW - Exosomes

KW - Pancreatic tissues

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JO - Pancreas

JF - Pancreas

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ER -

Exosomal markers (CD63 and CD9) expression pattern using immunohistochemistry in resected malignant and nonmalignant pancreatic specimens

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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