Exogenous nitric oxide prevents cardiovascular collapse during hemorrhagic shock

Parimala Nachuraju, Adam J. Friedman, Joel M. Friedman, Pedro Cabrales

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

This study investigated the systemic and microvascular hemodynamic changes related to increased nitric oxide (NO) availability following significant hemorrhage, made available by administration of NO releasing nanoparticles (NO-nps). Hemodynamic responses to hemorrhagic shock were studied in the hamster window chamber. Acute hemorrhage was induced by arterial controlled bleeding of 50% of blood volume, and the resulting hemodynamic parameters were followed over 90. min. Exogenous NO was administered in the form of NO-nps (5. mg/kg suspended in 50. μl saline) 10. min following induced hemorrhage. Control groups received equal dose of NO free nanoparticles (Control-nps) and Vehicle solution. Animals treated with NO-nps partially maintained systemic and microvascular function during hypovolemic shock compared to animals treated with Control-nps or the Vehicle (50. μl saline). The continuous NO released by the NO-nps reverted arteriolar vasoconstriction, partially recovered both functional capillary density and microvascular blood flows. Additionally, NO supplementation post hemorrhage prevented cardiac decompensation, and thereby maintained and stabilized the heart rate. Paradoxically, the peripheral vasodilation induced by the NO-nps did not decrease blood pressure, and combined with NO's effects on vascular resistance, NO-nps promoted intravascular pressure redistribution and blood flow, avoiding tissue ischemia. Therefore, by increasing NO availability with NO-nps during hypovolemic shock, it is possible that cardiac stability and microvascular perfusion can be preserved, ultimately increasing survivability and local tissue viability, and reducing hemorrhagic shock sequelae. The relevance, stability, and efficacy of exogenous NO therapy in the form of NO-nps will potentially facilitate the intended use in battlefield and trauma situations.

Original languageEnglish (US)
Pages (from-to)607-613
Number of pages7
JournalResuscitation
Volume82
Issue number5
DOIs
StatePublished - May 2011

Fingerprint

Hemorrhagic Shock
Nitric Oxide
Hemorrhage
Hemodynamics
Nanoparticles
Shock
Blood Pressure
Tissue Survival
Vasoconstriction
Blood Volume
Vasodilation
Cricetinae
Vascular Resistance

Keywords

  • Functional capillary density
  • Glutathione
  • Hemorrhage
  • Microcirculation
  • Nitrosoglutathione

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Emergency
  • Emergency Medicine

Cite this

Exogenous nitric oxide prevents cardiovascular collapse during hemorrhagic shock. / Nachuraju, Parimala; Friedman, Adam J.; Friedman, Joel M.; Cabrales, Pedro.

In: Resuscitation, Vol. 82, No. 5, 05.2011, p. 607-613.

Research output: Contribution to journalArticle

Nachuraju, Parimala ; Friedman, Adam J. ; Friedman, Joel M. ; Cabrales, Pedro. / Exogenous nitric oxide prevents cardiovascular collapse during hemorrhagic shock. In: Resuscitation. 2011 ; Vol. 82, No. 5. pp. 607-613.
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