Evolutionary gain of function for the ER membrane protein Sec62 from yeast to humans

Linda Müller, Maria Diaz De Escauriaza, Patrick Lajoie, Melanie Theis, Martin Jung, Anika Müller, Carsten Burgard, Markus Greiner, Erik L. Snapp, Johanna Dudek, Richard Zimmermann

Research output: Contribution to journalArticle

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Abstract

Because of similarity to their yeast orthologues, the two membrane proteins of the human endoplasmic reticulum (ER) Sec62 and Sec63 are expected to play a role in protein biogenesis in the ER. We characterized interactions between these two proteins as well as the putative interaction of Sec62 with ribosomes. These data provide further evidence for evolutionary conservation of Sec62/Sec63 interaction. In addition, they indicate that in the course of evolution Sec62 of vertebrates has gained an additional function, the ability to interact with the ribosomal tunnel exit and, therefore, to support cotranslational mechanisms such as protein transport into the ER. This view is supported by the observation that Sec62 is associated with ribosomes in human cells. Thus, the human Sec62/Sec63 complex and the human ER membrane protein ERj1 are similar in providing binding sites for BiP in the ER-lumen and binding sites for ribosomes in the cytosol. We propose that these two systems provide similar chaperone functions with respect to different precursor proteins.

Original languageEnglish (US)
Pages (from-to)691-703
Number of pages13
JournalMolecular Biology of the Cell
Volume21
Issue number5
DOIs
StatePublished - Mar 1 2010

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Endoplasmic Reticulum
Membrane Proteins
Yeasts
Ribosomes
Binding Sites
Protein Precursors
Protein Transport
Cytosol
Vertebrates
Proteins

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Müller, L., De Escauriaza, M. D., Lajoie, P., Theis, M., Jung, M., Müller, A., ... Zimmermann, R. (2010). Evolutionary gain of function for the ER membrane protein Sec62 from yeast to humans. Molecular Biology of the Cell, 21(5), 691-703. https://doi.org/10.1091/mbc.E09-08-0730

Evolutionary gain of function for the ER membrane protein Sec62 from yeast to humans. / Müller, Linda; De Escauriaza, Maria Diaz; Lajoie, Patrick; Theis, Melanie; Jung, Martin; Müller, Anika; Burgard, Carsten; Greiner, Markus; Snapp, Erik L.; Dudek, Johanna; Zimmermann, Richard.

In: Molecular Biology of the Cell, Vol. 21, No. 5, 01.03.2010, p. 691-703.

Research output: Contribution to journalArticle

Müller, L, De Escauriaza, MD, Lajoie, P, Theis, M, Jung, M, Müller, A, Burgard, C, Greiner, M, Snapp, EL, Dudek, J & Zimmermann, R 2010, 'Evolutionary gain of function for the ER membrane protein Sec62 from yeast to humans', Molecular Biology of the Cell, vol. 21, no. 5, pp. 691-703. https://doi.org/10.1091/mbc.E09-08-0730
Müller L, De Escauriaza MD, Lajoie P, Theis M, Jung M, Müller A et al. Evolutionary gain of function for the ER membrane protein Sec62 from yeast to humans. Molecular Biology of the Cell. 2010 Mar 1;21(5):691-703. https://doi.org/10.1091/mbc.E09-08-0730
Müller, Linda ; De Escauriaza, Maria Diaz ; Lajoie, Patrick ; Theis, Melanie ; Jung, Martin ; Müller, Anika ; Burgard, Carsten ; Greiner, Markus ; Snapp, Erik L. ; Dudek, Johanna ; Zimmermann, Richard. / Evolutionary gain of function for the ER membrane protein Sec62 from yeast to humans. In: Molecular Biology of the Cell. 2010 ; Vol. 21, No. 5. pp. 691-703.
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