Abstract
Characterization of residual plasma virus during antiretroviral therapy (ART) is a high priority to improve understanding of HIV-1 pathogenesis and therapy. To understand the evolution of HIV-1 pol and env genes in viremic patients under selective pressure of ART, we performed longitudinal analyses of plasma-derived pol and env sequences from single HIV-1 genomes. We tested the hypotheses that drug resistance in pol was unrelated to changes in coreceptor usage (tropism), and that recombination played a role in evolution of viral strains. Recombinants were identified by using Bayesian and other computational methods. High-level genotypic resistance was seen in ~70% of X4 and R5 strains during ART. There was no significant association between resistance and tropism. Each patient displayed at least one recombinant encompassing env and representing a change in predicted tropism. These data suggest that, in addition to mutation, recombination can play a significant role in shaping HIV-1 evolution.
Original language | English (US) |
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Pages (from-to) | 5-20 |
Number of pages | 16 |
Journal | Virology |
Volume | 404 |
Issue number | 1 |
DOIs | |
State | Published - Aug 2010 |
Keywords
- HIV-1 drug resistance
- HIV-1 recombination
- HIV-1 tropism
ASJC Scopus subject areas
- Virology