TY - JOUR
T1 - Evidence that the nonsense-mediated mRNA decay pathway participates in X chromosome dosage compensation in mammals
AU - Yin, Shanye
AU - Deng, Wenjun
AU - Zheng, Hancheng
AU - Zhang, Zhengguo
AU - Hu, Landian
AU - Kong, Xiangyin
N1 - Funding Information:
This work was supported by the National Natural Science Foundation of China (No. 30530450, No. 30871356), the National High Technology Research and Development Program of China (2006AA02Z330, 2006AA02A301), the National Basic Research Program of China (Nos. 2007CB512202, 2004CB518603), and the Knowledge Innovation Program of the Chinese Academy of Sciences (Grant No. KSCX1-YW-R-74).
PY - 2009/6/5
Y1 - 2009/6/5
N2 - Current models of X chromosome dosage compensation are usually framed by reference to how regulation in transcriptional level elevates the gene expression of the active X chromosome. This framework, however, might be oversimplified because regulation of gene expression can also act at the post-transcriptional level. Here, after a genome-wide survey, we find that autosomal genes are more likely subject to nonsense-mediated mRNA decay (NMD) than X-linked genes. Furthermore, we demonstrate that after NMD inhibition, balanced gene expression between X chromosome and autosomes is corrupted such that the global mean X/autosome gene expression ratio is decreased by 10-15%. Our results identify NMD as a post-transcription-level regulatory mechanism that contributes to the observed fine-tuning of X chromosome dosage compensation in mammals.
AB - Current models of X chromosome dosage compensation are usually framed by reference to how regulation in transcriptional level elevates the gene expression of the active X chromosome. This framework, however, might be oversimplified because regulation of gene expression can also act at the post-transcriptional level. Here, after a genome-wide survey, we find that autosomal genes are more likely subject to nonsense-mediated mRNA decay (NMD) than X-linked genes. Furthermore, we demonstrate that after NMD inhibition, balanced gene expression between X chromosome and autosomes is corrupted such that the global mean X/autosome gene expression ratio is decreased by 10-15%. Our results identify NMD as a post-transcription-level regulatory mechanism that contributes to the observed fine-tuning of X chromosome dosage compensation in mammals.
KW - Alternative splicing
KW - Nonsense-mediated mRNA decay (NMD)
KW - X chromosome dosage compensation
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U2 - 10.1016/j.bbrc.2009.04.021
DO - 10.1016/j.bbrc.2009.04.021
M3 - Article
C2 - 19364502
AN - SCOPUS:67349173946
SN - 0006-291X
VL - 383
SP - 378
EP - 382
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -