Evidence that a single replication fork proceeds from early to late replicating domains in the IgH locus in a non-B cell line

Olga V. Ermakova, Lam H. Nguyen, Randall D. Little, Christophe Chevillard, Roy Riblet, Nasrin Ashouian, Barbara K. Birshtein, Carl L. Schildkraut

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In non-B cell lines, like the murine erythroleukemia cell line (MEL), the most distal IgH constant region gene, Cα, replicates early in S; other heavy chain constant region genes, joining and diversity segments, and the most proximal Vh gene replicate successively later in S in a 3' to 5' direction proportional to their distance from Cα. In MEL, replication forks detected in the IgH locus also proceed in the same 3' to 5' direction for ~400 kb, beginning downstream of the IgH 3' regulatory region and continuing to the D region, as well as within the Vh81X gene. Downstream of the initiation region is an early replicating domain, and upstream of Vh81X is a late replicating domain. Hence, the gradual transition between early and late replicated domains can be achieved by a single replication fork.

Original languageEnglish (US)
Pages (from-to)321-330
Number of pages10
JournalMolecular Cell
Issue number3
Publication statusPublished - Mar 1999


ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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