Evidence that a single replication fork proceeds from early to late replicating domains in the IgH locus in a non-B cell line

Olga V. Ermakova, Lam H. Nguyen, Randall D. Little, Christophe Chevillard, Roy Riblet, Nasrin Ashouian, Barbara K. Birshtein, Carl L. Schildkraut

Research output: Contribution to journalArticle

65 Citations (Scopus)

Abstract

In non-B cell lines, like the murine erythroleukemia cell line (MEL), the most distal IgH constant region gene, Cα, replicates early in S; other heavy chain constant region genes, joining and diversity segments, and the most proximal Vh gene replicate successively later in S in a 3' to 5' direction proportional to their distance from Cα. In MEL, replication forks detected in the IgH locus also proceed in the same 3' to 5' direction for ~400 kb, beginning downstream of the IgH 3' regulatory region and continuing to the D region, as well as within the Vh81X gene. Downstream of the initiation region is an early replicating domain, and upstream of Vh81X is a late replicating domain. Hence, the gradual transition between early and late replicated domains can be achieved by a single replication fork.

Original languageEnglish (US)
Pages (from-to)321-330
Number of pages10
JournalMolecular Cell
Volume3
Issue number3
DOIs
StatePublished - Mar 1999

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Cell Line
Leukemia, Erythroblastic, Acute
Genes
Nucleic Acid Regulatory Sequences
Direction compound

ASJC Scopus subject areas

  • Molecular Biology

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Evidence that a single replication fork proceeds from early to late replicating domains in the IgH locus in a non-B cell line. / Ermakova, Olga V.; Nguyen, Lam H.; Little, Randall D.; Chevillard, Christophe; Riblet, Roy; Ashouian, Nasrin; Birshtein, Barbara K.; Schildkraut, Carl L.

In: Molecular Cell, Vol. 3, No. 3, 03.1999, p. 321-330.

Research output: Contribution to journalArticle

Ermakova, Olga V. ; Nguyen, Lam H. ; Little, Randall D. ; Chevillard, Christophe ; Riblet, Roy ; Ashouian, Nasrin ; Birshtein, Barbara K. ; Schildkraut, Carl L. / Evidence that a single replication fork proceeds from early to late replicating domains in the IgH locus in a non-B cell line. In: Molecular Cell. 1999 ; Vol. 3, No. 3. pp. 321-330.
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AU - Ermakova, Olga V.

AU - Nguyen, Lam H.

AU - Little, Randall D.

AU - Chevillard, Christophe

AU - Riblet, Roy

AU - Ashouian, Nasrin

AU - Birshtein, Barbara K.

AU - Schildkraut, Carl L.

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