Evidence of increased oxidative stress in aged mesenteric lymphatic vessels

Sangeetha Thangaswamy, Eric A. Bridenbaugh, Anatoliy A. Gashev

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Background: We have previously shown that aging is associated with weakened rat mesenteric lymphatic vessel (MLV) contractility. However, the specific mechanisms contributing to this aging-associated contractile degeneration remain unknown. Aging is often associated with elevations in oxidative stress, and reactive oxygen species (ROS) have been shown to reduce the contractility of MLV. Thus in the present study, we sought to assess whether aging is associated with increased levels of oxidative stress and oxidative damage in MLV. Methods and Results: MLV were isolated from 9-mo- and 24-mo-old Fischer-344 rats and subjected to the following experimental techniques: measurement of total superoxide dismutase (SOD) activity; estimation of lipid peroxidation levels via measurement of thiobarbituric acid reactive substances (TBARS); detection of superoxide and mitochondrial ROS in live MLV; Western blot analysis, and immunohistochemical labeling of the SOD isoforms and nitro-tyrosine proteins. We found that aging is associated with increased levels of cellular superoxide and mitochondrial ROS concomitant with a reduction in Cu/Zn-SOD protein expression and total SOD enzymatic activity in MLV. This increase in oxidative stress and decrease in antioxidant activity was associated with evidence of increased lipid (as indicated by TBARS) and protein (as indicated by nitro-tyrosine labeling) oxidative damage. Conclusions: Thus for the first time, we demonstrate that aging-associated increases in oxidative stress and oxidative damage is indeed present in the walls of MLV and may contribute to the aging-associated lymphatic pump dysfunction we previously reported.

Original languageEnglish (US)
Pages (from-to)53-62
Number of pages10
JournalLymphatic Research and Biology
Volume10
Issue number2
DOIs
StatePublished - Jun 1 2012
Externally publishedYes

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Lymphatic Vessels
Oxidative Stress
Superoxide Dismutase
Reactive Oxygen Species
Thiobarbituric Acid Reactive Substances
Superoxides
Tyrosine
Proteins
Inbred F344 Rats
Lipid Peroxidation
Protein Isoforms
Antioxidants
Western Blotting
Lipids

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Evidence of increased oxidative stress in aged mesenteric lymphatic vessels. / Thangaswamy, Sangeetha; Bridenbaugh, Eric A.; Gashev, Anatoliy A.

In: Lymphatic Research and Biology, Vol. 10, No. 2, 01.06.2012, p. 53-62.

Research output: Contribution to journalArticle

Thangaswamy, Sangeetha ; Bridenbaugh, Eric A. ; Gashev, Anatoliy A. / Evidence of increased oxidative stress in aged mesenteric lymphatic vessels. In: Lymphatic Research and Biology. 2012 ; Vol. 10, No. 2. pp. 53-62.
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