Evidence of Diversity, Site, and Host Specificity of Sea Turtle Blood Flukes (Digenea: Schistosomatoidea: "spirorchiidae"): A Molecular Prospecting Study

Brian A. Stacy, Phoebe A. Chapman, Allen M. Foley, Ellis C. Greiner, Lawrence H. Herbst, Alan B. Bolten, Paul A. Klein, Charles A. Manire, Elliott R. Jacobson

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Neospirorchis (Digenea: "Spirorchiidae") are blood flukes of sea turtles. Trematodes tentatively identified as Neospirorchis sp. infect various sites within sea turtles inhabiting waters of the southeastern United States, but efforts to obtain specimens adequate for morphologic study has proven difficult. Two genetic targets, the internal transcribed spacer region of the ribosomal RNA gene and the partial mitochondrial cytochrome c oxidase subunit I gene, were used to investigate potential diversity among parasite specimens collected from stranded sea turtles. Sequence data were obtained from 215 trematode and egg specimens collected from 92 individual free-ranging cheloniid sea turtles comprising 4 host species. Molecular analysis yielded more than 20 different genotypes. We were able to assign 1 genotype to 1 of the 2 recognized species, Neospirorchis pricei Manter and Larson, 1950. In many examples, genotypes exhibited host and site specificity. Our findings indicate considerable diversity of parasites resembling Neospirorchis with evidence of a number of uncharacterized blood flukes that require additional study.

Original languageEnglish (US)
Pages (from-to)756-767
Number of pages12
JournalJournal of Parasitology
Volume103
Issue number6
DOIs
StatePublished - Dec 1 2017

Fingerprint

blood flukes
Trematoda
Turtles
Digenea (Trematoda)
host specificity
Host Specificity
sea turtles
turtle
blood
genotype
Genotype
parasite
Parasites
Southeastern United States
parasites
gene
molecular analysis
Electron Transport Complex IV
rRNA Genes
cytochrome-c oxidase

ASJC Scopus subject areas

  • Parasitology
  • Ecology, Evolution, Behavior and Systematics

Cite this

Evidence of Diversity, Site, and Host Specificity of Sea Turtle Blood Flukes (Digenea : Schistosomatoidea: "spirorchiidae"): A Molecular Prospecting Study. / Stacy, Brian A.; Chapman, Phoebe A.; Foley, Allen M.; Greiner, Ellis C.; Herbst, Lawrence H.; Bolten, Alan B.; Klein, Paul A.; Manire, Charles A.; Jacobson, Elliott R.

In: Journal of Parasitology, Vol. 103, No. 6, 01.12.2017, p. 756-767.

Research output: Contribution to journalArticle

Stacy, Brian A. ; Chapman, Phoebe A. ; Foley, Allen M. ; Greiner, Ellis C. ; Herbst, Lawrence H. ; Bolten, Alan B. ; Klein, Paul A. ; Manire, Charles A. ; Jacobson, Elliott R. / Evidence of Diversity, Site, and Host Specificity of Sea Turtle Blood Flukes (Digenea : Schistosomatoidea: "spirorchiidae"): A Molecular Prospecting Study. In: Journal of Parasitology. 2017 ; Vol. 103, No. 6. pp. 756-767.
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