Evidence for two classes of 1,25-dihydroxyvitamin D3 binding sites in classical vs. nonclassical target tissues

Robert C. Gensure; Paul C. Riggle; Steve D. Antrobus; Marian R. Walters

doi:10.1016/S0006-291X(05)81145-3

Evidence for two classes of 1,25-dihydroxyvitamin D₃ binding sites in classical vs. nonclassical target tissues

Robert C. Gensure, Paul C. Riggle, Steve D. Antrobus, Marian R. Walters

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

Possible differences in 1,25-Dihydroxyvitamin D₃ [1,25(OH)₂D₃] binding sites in classical and nonclassical target tissues were tested by Scatchard analysis of [³H]1,25(OH)₂D₃ binding in parallel chromatin preparations of rat kidney vs. testis. Two distinct binding components were resolved in kidney (p<0.005). Moreover, the single binding site in testis exhibited a 10-fold lower K_d (p<0.05) than did the principal binding site in kidney (50±4 vs. 405±142 pM). Secondly, regulation of [³H]1,25(OH)₂D₃ binding sites also differed. 1,25(OH)₂D₃ injection resulted in increased 1,25(OH)₂D₃ binding (p<0.05) in kidney (92%) and intestine (415%),but not in testis, lung or heart. These results suggest that the principal 1,25(OH)₂D₃ binding sites in classical targets kidney and intestine may be intrinsically different from those in at least some nonclassical targets.

Original language	English (US)
Pages (from-to)	867-873
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	180
Issue number	2
DOIs	https://doi.org/10.1016/S0006-291X(05)81145-3
State	Published - Oct 31 1991
Externally published	Yes

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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title = "Evidence for two classes of 1,25-dihydroxyvitamin D3 binding sites in classical vs. nonclassical target tissues",

abstract = "Possible differences in 1,25-Dihydroxyvitamin D3 [1,25(OH)2D3] binding sites in classical and nonclassical target tissues were tested by Scatchard analysis of [3H]1,25(OH)2D3 binding in parallel chromatin preparations of rat kidney vs. testis. Two distinct binding components were resolved in kidney (p<0.005). Moreover, the single binding site in testis exhibited a 10-fold lower Kd (p<0.05) than did the principal binding site in kidney (50±4 vs. 405±142 pM). Secondly, regulation of [3H]1,25(OH)2D3 binding sites also differed. 1,25(OH)2D3 injection resulted in increased 1,25(OH)2D3 binding (p<0.05) in kidney (92%) and intestine (415%),but not in testis, lung or heart. These results suggest that the principal 1,25(OH)2D3 binding sites in classical targets kidney and intestine may be intrinsically different from those in at least some nonclassical targets.",

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AU - Riggle, Paul C.

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AU - Walters, Marian R.

PY - 1991/10/31

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N2 - Possible differences in 1,25-Dihydroxyvitamin D3 [1,25(OH)2D3] binding sites in classical and nonclassical target tissues were tested by Scatchard analysis of [3H]1,25(OH)2D3 binding in parallel chromatin preparations of rat kidney vs. testis. Two distinct binding components were resolved in kidney (p<0.005). Moreover, the single binding site in testis exhibited a 10-fold lower Kd (p<0.05) than did the principal binding site in kidney (50±4 vs. 405±142 pM). Secondly, regulation of [3H]1,25(OH)2D3 binding sites also differed. 1,25(OH)2D3 injection resulted in increased 1,25(OH)2D3 binding (p<0.05) in kidney (92%) and intestine (415%),but not in testis, lung or heart. These results suggest that the principal 1,25(OH)2D3 binding sites in classical targets kidney and intestine may be intrinsically different from those in at least some nonclassical targets.

AB - Possible differences in 1,25-Dihydroxyvitamin D3 [1,25(OH)2D3] binding sites in classical and nonclassical target tissues were tested by Scatchard analysis of [3H]1,25(OH)2D3 binding in parallel chromatin preparations of rat kidney vs. testis. Two distinct binding components were resolved in kidney (p<0.005). Moreover, the single binding site in testis exhibited a 10-fold lower Kd (p<0.05) than did the principal binding site in kidney (50±4 vs. 405±142 pM). Secondly, regulation of [3H]1,25(OH)2D3 binding sites also differed. 1,25(OH)2D3 injection resulted in increased 1,25(OH)2D3 binding (p<0.05) in kidney (92%) and intestine (415%),but not in testis, lung or heart. These results suggest that the principal 1,25(OH)2D3 binding sites in classical targets kidney and intestine may be intrinsically different from those in at least some nonclassical targets.

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Evidence for two classes of 1,25-dihydroxyvitamin D₃ binding sites in classical vs. nonclassical target tissues

Abstract

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