Evidence for two classes of 1,25-dihydroxyvitamin D3 binding sites in classical vs. nonclassical target tissues

Robert C. Gensure, Paul C. Riggle, Steve D. Antrobus, Marian R. Walters

Research output: Contribution to journalArticle

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Abstract

Possible differences in 1,25-Dihydroxyvitamin D3 [1,25(OH)2D3] binding sites in classical and nonclassical target tissues were tested by Scatchard analysis of [3H]1,25(OH)2D3 binding in parallel chromatin preparations of rat kidney vs. testis. Two distinct binding components were resolved in kidney (p<0.005). Moreover, the single binding site in testis exhibited a 10-fold lower Kd (p<0.05) than did the principal binding site in kidney (50±4 vs. 405±142 pM). Secondly, regulation of [3H]1,25(OH)2D3 binding sites also differed. 1,25(OH)2D3 injection resulted in increased 1,25(OH)2D3 binding (p<0.05) in kidney (92%) and intestine (415%),but not in testis, lung or heart. These results suggest that the principal 1,25(OH)2D3 binding sites in classical targets kidney and intestine may be intrinsically different from those in at least some nonclassical targets.

Original languageEnglish (US)
Pages (from-to)867-873
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume180
Issue number2
DOIs
Publication statusPublished - Oct 31 1991

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ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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